Megazyme代理 K-ASNAM L-天冬酰胺酸/L-谷氨酸/氨[快速]检测试剂盒

L-天冬酰胺/ L-谷氨酰胺/氨检测试剂盒（快速）
英文名：L-Asparagine/L-Glutamine/Ammonia Assay Kit (Rapid)
货号：K-ASNAM
规格：100 assays (50 of each) per kit

The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.

UV-method for the determination of L-Asparagine, L-Glutamine 
and Ammonia in potatoes, foodstuffs and cell culture media

Principle:
(glutaminase)
(1) L-Glutamine + H₂O → L-glutamate + NH₄⁺

(microbial glutamate dehydrogenase)
(2) NH₄⁺ + 2-oxoglutarate + NADPH → L-glutamate + NADP⁺ + H₂O

(asparaginase)
(3) L-Asparagine + H₂O → L-aspartate + NH₄⁺

Kit size:                              50 assays of each
Method:                              Spectrophotometric at 340 nm
Reaction time:                   ~ 20 min
Detection limit:                  0.50 mg/L (L-asparagine)
0.54 mg/L (L-glutamine)
0.06 mg/L (ammonia)
Application examples:
Potatoes, potato products, vegetables, cereals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:          Novel method

Advantages

• Very rapid reaction due to use of uninhibited glutamate dehydrogenase
• All enzymes supplied as stabilised suspensions
• Only kit available
• Very cost effective
• All reagents stable for > 2 years after preparation
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included

L-天冬酰胺/L-谷氨酰胺/氨检测试剂盒是一种用于食品工业中作为丙烯酰胺前体的L-天冬酰胺、L-谷氨酰胺、L-谷氨酰胺、L-天冬酰胺、L-谷氨酰胺、L-天冬酰胺、L-谷氨酰胺、L-天冬酰胺/L

扩展协因子稳定性溶解辅助性因子在4时稳定>1年^oC.

紫外分光光度法测定L-天冬酰胺L-谷氨酰胺 
土豆、食品和细胞培养基中的氨

原则：
(谷氨酰胺酶)
(1)L-谷氨酰胺+H₂O→L-谷氨酸+NH₄⁺

(微生物谷氨酸脱氢酶)
(2)NH₄⁺ +2-氧戊二酸+NADPH→L-谷氨酸+NADP⁺ +H₂O

(天冬酰胺酶)
(3)L-天冬酰胺+H₂O→L-天冬氨酸+NH₄⁺