Megazyme代理 K-AMYL Amylose/Amylopectin Assay Kit
直链淀粉/支链淀粉[胶淀粉]检测试剂盒
英文名：Amylose/Amylopectin Assay Kit
货号：K-AMYL
规格：100 assays per kit
品牌： Megazyme
For the measurement of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure. Content:100 assays per kit
Colourimetric method for the determination of Amylose and
Amylopectin in cereals, food and feed
Principle:
(Con A)
(1) Soluble starch (amylose + amylopectin) →
amylose + amylopectin-Con A
(soluble) (precipitate)
(α-amylase + amyloglucosidase)
(2) Amylose (in solution) + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + H2O
(α-amylase + amyloglucosidase)
(5) Total starch (in solution) + H2O → D-glucose
Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 120 min
Detection limit: Amylose 5-95% of total starch content
Application examples:
Cereal starches, flours, pure starches and foods
Method recognition: Novel method
Advantages
- Very cost effective (cost per test)
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Accurate and reliable amylose / amylopectin ratio determination
- Simple format
- Standard included
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Amylopectin, Amylose |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Signal Response: | Increase |
| Limit of Detection: | Amylose 5-95% of total starch content |
| Total Assay Time: | ~ 120 min |
| Application examples: | Cereal starches, flours, pure starches and foods. |
| Method recognition: | Novel method |
The Amylose/Amylopectin test kit is suitable for the measurement and analysis of amylose/amylopectin ratio and content in cereal starches and flours. Based on a Con A precipitation procedure.
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O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
O-PAN-1G – Panose
O-IPAN – Isopanose
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