Megazyme呈色底物
爱尔兰Megazyme是一家制造和供应高品质和创新的检测谷物，食品，饲料，发酵，乳制品和葡萄酒行业的技术性企业。Megazyme成立于1988年，该公司目前提供了超过70种试剂盒和超过300种其他试剂和底物。
1. The substrate is water soluble and is readily recognised and hydrolysed by an endo-acting hydrolase.
2. The reaction is terminated by the addition of a precipitant solution (e.g. ethanol) wherein high molecular weight, partially hydrolysed substrate fragments precipitate from solution.
3. The suspension is mixed thoroughly, centrifuged, and the colour in the solution is measured using a spectrophotometer.
4. The enzymatic activity is determined using a standard curve provided.
Our range of soluble dyed substrates
| Product Name | Product Code | Enzyme Measured | |
| Red Starch | S-RSTAR | α-Amylase | |
| Red Debranched Arabinan (sugar beet) | S-RDAR | endo-1,5-α-Arabinanase | |
| Azo-CM-Cellulose (liquid) | S-ACMCL | ||
| Azo-CM-Cellulose (powder) | S-ACMC | endo-Cellulase / endo-1,4-β-Glucanase | |
| Azo-Xyloglucan (tamarind) | S-AZXG | ||
| Azo-Barley Glucan | S-ABG100 | Lichenase / endo-b-Glucanase | |
| Azo-Fructan | S-AZFR5 | endo-Fructanase / endo-Inulinase | |
| Azo-Fructan plus exo-Inulinase | S-AZFRXOI | ||
| Red Pullulan | S-RPUL | Pullulanase / Limit-dextrinase | |
| Azo-Carob Galactomannan | S-ACGLM | endo-1,4-β-Mannanase | |
| Azo-Galactan (potato) | S-AGALP | endo-1,4-b-Galactanase | |
| Azo-Casein (sulphanilamide dyed) | S-AZCAS | endo-Protease | |
| AZ-Rhamnogalacturonan | S-AZRH | endo-Rhamnogalacturonan hydrolase / lyase | |
| Azo-Wheat Arabinoxylan (liquid) | S-AWAXL | ||
| Azo-Wheat Arabinoxylan (powder) | S-AWAXP | endo-1,4-b-Xylanase | |
| Azo-Xylan (birchwood) (liquid) | S-AXBL | ||
| Azo-Xylan (birchwood) (powder) | S-AXBP | ||
b) Insoluble Dyed Substrates
Megazyme’s insoluble dyed substrates are prepared by dyeing and crosslinking soluble polysaccharides.
These substrates are provided in both granular and fine particle size. The finely milled substrates are more useful in agar plate assays and semi quantitative tube and plate assays. Substrates in other colours are also being developed for gel/agar plate activity screenings.
Insoluble chromogenic substrates are recommended for semi-quantitative assays.
Key features of Megazyme’s insoluble dyed substrates
Suitable for analysis of crude enzyme extracts Quantitative assay
Suitable for analysis of purified enzymes Chemically defined substrate
Suitable for gel/agar plate activity screening Suitable for automation
Suitable for endo-acting enzymes Suitable for exo-acting enzymes
Assay procedure for insoluble dyed substrates
1.
The hydrated insoluble substrate forms gelatinous particles which are readily recognised and hydrolysed by an endo-acting hydrolase. The partial hydrolysis of insoluble chromogenic substrates releases water soluble dyed substrate fragments.
2.
The reaction is terminated by adding an alkaline solution to stop enzyme activity and the reaction slurry is filtered or centrifuged.
3.
The colour released in solution is measured using a spectrophotometer and the colour intensity is directly related to enzyme activity.
| Product Name | Product Code | Enzyme Measured | |
| AZCL-Amylose | I-AZAMY | ||
| AZCL-Amylose (fine) | I-AZAMYF | a-Amylase | |
| RedCL-Amylose (fine) | I-RCLAMYF | ||
| AZCL-Arabinan (debranched) | I-AZDAR | endo-1,5-α-Arabinanase | |
| AZCL-HE-Cellulose | I-AZCEL | ||
| AZCL-HE-Cellulose (fine) | I-AZCELF | ||
| RedCL-HE-Cellulose (fine) | I-RCLCELF | ||
| Azo-α-Cellulose | I-ACELL | endo-Cellulase | |
| Azo-Avicel | I-AAVIC | ||
| AZCL-Xyloglucan | I-AZXYG | ||
| AZCL-Xyloglucan (fine) | I-AZXYGF | ||
| RedCL-Xyloglucan (fine) | I-RCLXYGF | ||
| AZCL-Chitosan | I-AZCHAN | endo-Chitosanase | |
| AZCL-Chitosan (fine) | I-AZCHANF | ||
| AZCL-Dextran | I-AZDEX | endo-1,6-α-Dextrinase | |
| AZCL-Dextran (fine) | I-AZDEXF | ||
| AZCL-Galactan (potato) | I-AZGLP | endo-1,4-b-Galactanase | |
| AZCL-Galactan (potato) (fine) | I-AZGLPF | ||
| AZCL-Curdlan | I-AZCUR | ||
| AZCL-Curdlan (fine) | I-AZCURF | endo-1,3-b-Glucanase | |
| RedCL-Curdlan (fine) | I-RCLCURF | ||
| AZCL-Pachyman | I-AZPAC | ||
| AZCL-Barley b-Glucan | I-AZBGL | ||
| AZCL-Barley b-Glucan (fine) | I-AZBGLF | Lichenase / endo-b-Glucanase | |
| RedCL-Barley b-Glucan (fine) | I-RCLBGLF | ||
| AZCL-Galactomannan | I-AZGMA | ||
| AZCL-Galactomannan (fine) | I-AZGMAF | endo-1,4-b-Mannanase | |
| RedCL-Galactomannan (fine) | I-RCLGMAF | ||
| AZCL-Casein | I-AZCAS | endo-Protease | |
| AZCL-Collagen | I-AZCOL | ||
| AZCL-Pullulan | I-AZPUL | ||
| AZCL-Pullulan (fine) | I-AZPULF | Pullulanase / Limit-Dextrinase | |
| RedCL-Pullulan (fine) | I-RCLPULF | ||
| AZCL-Rhamnogalacturonan 1 | I-AZRHI | Rhamnogalacturonan hydrolase / lyase | |
| AZCL-Arabinoxylan (Wheat) | I-AZWAX | ||
| AZCL-Arabinoxylan (Wheat) (fine) | I-AZWAXF | endo-1,4-b-D-Xylanase | |
| AZCL-Xylan (Birchwood) | I-AZXBW | ||
| RedCL-Arabinoxylan (Wheat) (fine) | I-RCLWAXF | ||
c) Tablet Substrates
Megazyme supplies a range of enzyme tablet tests for ultimate end-user convenience. The insoluble chromogenic substrates discussed above are formulated into tablets. By using tablet tests, it is possible to obtain quantitative determinations of endo-acting hydrolase activities.
From a procedural perspective, tablet tests operate in the same way as assays using insoluble chromogenic substrates. The advantage of enzyme tablet tests is that the need to accurately weigh substrate quantities (and the error associated with this parameter) is removed.
Key features of Megazyme’s tablet substrates
Suitable for analysis of crude enzyme extracts Chemically defined substrate
Suitable for analysis of purified enzymes Suitable for automation
Quantitative assay Suitable for gel/agar plate activity screening
Suitable for endo-acting enzymes Suitable for exo-acting enzymes
Our range of tablet substrates
| Product Name | Product Code | Enzyme Measured | |
| Amylazyme | T-AMZ | ||
| Amylazyme BG | T-AMZBG | α-Amylase | |
| Amylazyme Red | T-AMZRD | ||
| Amylazyme HY | T-AMZHY | ||
| Arabinazyme | T-ARZ | endo-1,5-α-Arabinanase | |
| Cellazyme AF (40mg) | T-CAF | ||
| Cellazyme C (60mg) | T-CCZ | endo-Cellulase / endo-1,4-b-Glucanase | |
| Cellazyme T | T-CTZ | ||
| Chitozyme | T-CHZ | endo-Chitosanase | |
| α-Dextrazyme | T-DEXT | endo-1,6-α-Dextranase | |
| b-Glucazyme | T-BGZ | Lichenase / endo-b-Glucanase | |
| Galactazyme | T-GLZ | endo-1,4-b-Galactanase | |
| 1,3-b-Glucazyme | T-PAZ | endo-1,3-b-Glucanase | |
| 1,3-b-Glucazyme HS | T-CUR | ||
| Limit-Dextrizyme | T-LDZ | Pullulanase / Limit-dextrinase | |
| Mannazyme | T-MNZ | endo-1,4-b-Mannanase | |
| Protazyme AK | T-PRAK | endo-Protease | |
| Protazyme OL | T-PROL | ||
| Rhamnozyme | T-RHAM | Rhamnogalacturonan hydrolase / lyase | |
| Psyllazyme | T-PSYL | ||
| Xylazyme AF (40mg) | T-XAF | endo-1,4-b-Xylanase | |
| Xylazyme AX (60mg) | T-XAX | ||
| Xylazyme (100mg) | T-XYZ | ||
| Megazyme | S-ABG100 | 麦芽粉中葡聚糖酶检测底物 |
| Megazyme | S-ACGLM | 甘露聚糖酶检测底物 |
| Megazyme | S-AZFR5 | 内切型菊粉酶检测底物 |
| Megazyme | S-AZFRXOI | 内切型菊粉酶特殊检测底物 |
| Megazyme | S-AGALP | 半乳聚糖酶特殊检测底物 |
| Megazyme | S-AWAXL | 木聚糖酶检测底物 |
| Megazyme | S-AWAXP | 木聚糖酶检测底物(Powder) |
| Megazyme | S-AXBL | 木聚糖酶特殊检测底物 |
| Megazyme | S-AXBP | 木聚糖酶特殊检测底物 |
| Megazyme | S-AZXG | 纤维素酶可溶性发色检测底物 |
| Megazyme | S-AZRH | 鼠李聚糖半乳糖醛酸内切酶特殊检测底物 |
| Megazyme | S-RDAR | 阿拉伯聚糖检测底物 |
| Megazyme | S-RPUL | 酶制备中的普鲁兰酶和麦粉中的极限糊精的检测底物 |
| Megazyme | S-RSTAR | Alpha淀粉酶活性检测底物 |
| Megazyme | S-ACMC | 葡聚糖酶检测底物 |
| Megazyme | S-ACMCL | 葡聚糖酶检测底物(Liquid) |
| Megazyme | I-AZAMY | Alpha淀粉酶检测底物 |
| Megazyme | I-AZDAR | 1,5-a-L-阿拉伯聚糖内切酶检测底物 |
| Megazyme | I-AZWAX | 1，4-β-D-木聚糖内切酶检测底物 |
| Megazyme | I-AZBGL | 麦芽Beta葡聚糖酶、地衣聚糖酶和纤维素酶检测底物 |
| Megazyme | I-AZCAS | 蛋白酶检测底物 |
| Megazyme | I-AZCEL | 纤维素内切酶检测底物 |
| Megazyme | I-AZCHAN | 脱乙酰几丁质酶/脱乙酰壳多糖酶检测底物 |
| Megazyme | I-AZCOL | 蛋白酶检测底物 |
| Megazyme | I-ACELL | 纤维素内切酶检测底物 |
| Megazyme | I-AAVIC | 纤维素内切酶检测底物 |
| Megazyme | I-AZPUL | 极限糊精酶[普鲁兰酶]检测底物 |
| Megazyme | I-AZRHI | 鼠李聚糖半乳糖醛酸水解酶和裂解酶检测底物 |
| Megazyme | I-AZXYG | 纤维素内切酶检测底物 |
| Megazyme | I-AZBGLF | AZCL-大麦β-葡聚糖(精细的) |
| Megazyme | I-AZXBW | 1,4-β-D-木聚糖内切酶检测底物. |
| Megazyme | I-AZPAC | 1,3-β-D-葡聚糖内切酶检测底物 |
| Megazyme | I-AZGMA | 1,4-β-D-甘露聚糖内切酶检测底物 |
| Megazyme | I-AZGLP | 1,4-β-D-半乳糖内切酶检测底物 |
| Megazyme | I-AZDEX | 1,6-a-D-葡聚糖内切酶检测底物 |
| Megazyme | I-AZCUR | 1,3-β-D-葡聚糖内切酶检测底物 |
| Megazyme | I-AZGLL | AZCL-Galactan (Lupin) – 3g |
| Megazyme | I-AZLIC | AZCL-Lichenan 3g |
| Megazyme | I-AZXOT | AZCL-Xylan (Oat Spelt) 4g |
| Megazyme | I-RCLAMYF | RedCL-Amylose (fine) 5g |
| Megazyme | I-RCLCELF | RedCL-HE-Cellulose (fine) 3g |
| Megazyme | I-RCLXYGF | RedCL-Xyloglucan (fine) 4g |
| Megazyme | I-AZGLPF | AZCL-Galactan (Potato) (fine) 3g |
| Megazyme | I-RCLGMAF | RedCL-Galactomannan (fine) 3g |
| Megazyme | I-AZAMYF | AZCL-Amylose (fine) 5g |
| Megazyme | I-AZPULF | AZCL-Pullulan (fine) 3g |
| Megazyme | I-AZDEXF | AZCL-Dextran (fine) 3g |
| Megazyme | I-AZCURF | AZCL-Curdlan (fine) 3g |
| Megazyme | I-AZXYGF | AZCL-Xyloglucan (fine) 4g |
| Megazyme | I-AZGMAF | AZCL-Galactomannan (fine) 3g |
| Megazyme | I-AZWAXF | AZCL-Arabinoxylan (Wheat) (fine) 3g |
| Megazyme | I-AZXBE | AZCL-Xylan (Beechwood) |
| Megazyme | S-AZCAS | 蛋白酶底物（AZO-Casein)a |