Isoamylase (Glycogen 6-glucanohydrolase)-Megazyme试剂盒

Product code: E-ISAMY
€220.00

600 Units

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Description Documents (35) Related Products Publications (20) Safety Information

Content: 600 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: Isoamylase
EC Number: 3.2.1.68
CAZy Family: GH13
CAS Number: 9067-73-6
Synonyms: isoamylase; glycogen 6-alpha-D-glucanohydrolase
Source: Pseudomonas sp.
Molecular Weight: 71,500
Concentration: Supplied at ~ 200 U/mL
Expression: Purified from Pseudomonas sp.
Specificity: Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
Specific Activity: ~ 180 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg)
Unit Definition: One unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC.
Temperature Optima: 50oC
pH Optima: 4
Application examples: Applications in carbohydrate research and in the food and feeds, and cereals industry.
Method recognition: AOAC Method 2000.11 and GB Standard 5009.245-2016

Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Please refer to E-ISAMYHP for high purity enzyme suitable for use in starch structural research.

We have a wide range of other CAZyme products available.

Validation of Methods

Isoamylase (Glycogen 6-glucanohydrolase)Isoamylase (Glycogen 6-glucanohydrolase)

Documents
Certificate of Analysis
Select COA based on lot# 130102a 130103b 130103a

Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

FAQs Data Sheet

Related products
Similar Products
E-ISAMYHP – Isoamylase HP (Glycogen 6-glucanohydrolase)

Related Carbohydrates
O-IPAN – Isopanose O-GMT – 63-α-D-Glucosyl-maltotriose O-PAN-1G – Panose P-BLDX-10G – β-Limit Dextrin P-GLYAL – Glycogen (Algae)

Associated Enzymes
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-BARBL-50KU – β-Amylase (Barley) E-PULKP – Pullulanase M1 (Klebsiella planticola) E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)

Related Products
K-TDFR-200A – Total Dietary Fiber Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-TSTA-100A – Total Starch Assay Kit (AA/AMG) K-AMYL – Amylose/Amylopectin Assay Kit

Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Isoamylase (Glycogen 6-glucanohydrolase)

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