β-Glucuronidase (Escherichia coli)-Megazyme试剂盒

Product code: E-BGLAEC
€209.00

500,000 Units

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Description Documents (36) Related Products Publications (2) Safety Information

Content: 500,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In solution (Tris.HCl/NaCl/EDTA)
Physical Form: Solution
Stability: > 4 years at 4oC
Enzyme Activity: β-Glucuronidase
EC Number: 3.2.1.31
CAZy Family: GH2
CAS Number: 9001-45-0
Synonyms: β-D-glucuronoside glucuronosohydrolase; GUS
Source: Escherichia coli
Molecular Weight: 82,600
Concentration: Supplied at ~ 250 kU/mL
Expression: Recombinant from Escherichia coli
Specificity: Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.
Specific Activity: ~ 15,000 U/mg (37oC, pH 6.8 on phenolphthalein-β-D-glucuronide);
~ 50 U/mg (37oC, pH 7.5 on pNP-β-D-glucuronide)
Unit Definition: 30000 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µg of phenolphthalein per hour from phenolphthalein-β-D-glucuronide (0.5 mM) in sodium phosphate buffer (100 mM) at pH 6.8 and 37oC.
110 U/mg protein: One Unit of β-D-glucuronosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from pNP-β-D-glucuronide (1 mM) in Tris.HCl buffer (100 mM) pH 7.5 and 37oC., monitored at 410 nm.
Temperature Optima: 37oC
pH Optima: 6.8

High purity recombinant β-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Do Not Freeze/Thaw.

Looking for other enzymes? Browse our Carbohydrate Active enZYme and glycobiology enzyme products lists.

Documents
Certificate of Analysis
Select COA based on lot# 120503a 120502c 120502b 120502a 120501

Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Data Sheet

Related products
Colourimetric Substrates
O-PNPBGA – 4-Nitrophenyl-β-D-glucuronide

Related Products
K-URONIC – D-Glucuronic/D-Galacturonic Acid Assay Kit E-AGUBS – α-Glucuronidase (Geobacillus stearothermophilus)

Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

β-Glucuronidase (Escherichia coli)

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