Galactose dehydrogenase – Galactose mutarotase-Megazyme试剂盒

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Product code: E-GALMUT
€215.00

1 mL; galactose dehydrogenase (200 U/mL) /
galactose mutarotase (4 mg/mL)

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Description Documents (36) Related Products Publications (2) Safety Information

Content: 1 mL; galactose dehydrogenase (200 U/mL) / galactose mutarotase (4 mg/mL)
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: Dehydrogenase
EC Number: Galactose dehydrogenase: 1.1.1.48
Galactose mutarotase: 5.1.3.3
CAS Number: Galactose dehydrogenase: 9028-54-0
Galactose mutarotase: 9031-76-9
Synonyms: Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase
Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase
Source: Escherichia coli
Expression: Recombinant from Escherichia coli
Specificity: Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1). 

(1) α-D-Galactose ↔ β-D-Galactose.

The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2).

(2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+

Specific Activity: Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25oC
Galactose mutarotase: 4.1 mg/mL
Unit Definition: One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at pH 8.6, at 25oC.
Temperature Optima: 25oC
pH Optima: 8.6
Application examples: Used for the rapid measurement of D-galactose or L-arabinose in the L-Arabinose/D-Galactose Assay Kit (K-ARGA). Also used in the measurement of lactose via D-galactose in the Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR).

High purity Galactose dehydrogenase (Escherichia coli) / Galactose mutarotase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Can be employed for the rapid determination of D-galactose or L-arabinose. See Lactose/D-Galactose (Rapid) Assay Kit (K-LACGAR) for full details.

For use under Patent No. US 7,785,771 B2 and EP1 828 407 (GB, FR, IE, DE)

Browse a wide range of enzymes for analytical applications.

Documents
Certificate of Analysis
Select COA based on lot# 180901 130402c 130402b 130402 130401

Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Data Sheet

Related products
Similar Products
E-GALDH – Galactose dehydrogenase (soil prokaryote)

Related Products
K-ARGA – L-Arabinose/D-Galactose Assay Kit C-NAD-5G – β-Nicotinamide Adenine Dinucleotide (NAD+), Oxidised

Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Galactose dehydrogenase - Galactose mutarotase

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