Fructanase Mixture (Ultrapure, recombinant, powder)-Megazyme试剂盒

Product code: E-FRPDPU
€276.00

20,000 Units

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Description Documents (32) Safety Information

Content: 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: Supplied as a freeze-dried powder
Physical Form: Powder
Stability: Minimum 1 year at < -10oC. Check vial for details.
Enzyme Activity: Fructanase
EC Number: endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
CAZy Family: GH32
CAS Number: 9001-57-4,
9025-67-6,
37288-56-5
Synonyms: endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Source: Aspergillus niger
Concentration: endo-inulinase: Supplied at ~ 100 U/mL
exo-inulinase: Supplied at ~ 2,000 U/mL
Expression: Recombinant from Aspergillus niger
Specificity: endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Unit Definition: endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
Temperature Optima: 60oC
pH Optima: 4.5

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Componentsexo-Inulinase 20,000 U (on kestose at 40oC), endo-inulinase 1000 U (on fructan at 40oC).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

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Validation of Methods

Fructanase Mixture   (Ultrapure, recombinant, powder)

Documents

Certificate of Analysis

Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Data Sheet

Safety Information
Symbol : GHS08
Signal Word : Danger
Hazard Statements : H334
Precautionary Statements : P261, P284, P304+P340, P342+P311, P501
Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Fructanase Mixture   (Ultrapure, recombinant, powder)

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