Fructanase Mixture (powder)-Megazyme试剂盒

Product code: E-FRMXPD
€212.00

20,000 Units

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Description Documents (36) Related Products Publications (3) Safety Information

Content: 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: Supplied as a lyophilised powder
Physical Form: Powder
Stability: Minimum 1 year at < -10oC. Check vial for details.
Enzyme Activity: Fructanase
EC Number: endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
CAZy Family: GH32
CAS Number: 9001-57-4,
9025-67-6,
37288-56-5
Synonyms: endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Source: Aspergillus sp.
Expression: From Aspergillus sp.
Specificity: endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Unit Definition: endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
Temperature Optima: 40oC
pH Optima: 4.5
Method recognition: AOAC Method 997.08, AOAC Method 2000.11, AOAC Method 2016.06, GB Standard 5009.245-2016 and GB Standard 5009.255-2016

Powder (freeze dried)

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Componentsexo-inulinase 20,000 U (on kestose, at 40oC), endo-inulinase 1000 U (on fructan at 40oC), α-galactosidase 0.70 U (on p-nitrophenyl α-galactoside at 40oC), β-Glucanase 1.25 U (on β-glucan at 40oC) and pectinase 5.5 U (on pectin at 40oC).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Fructanase and sucrase (E-SUCR) are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.

See more of our other Carbohydrate Active enZYmes products.

Validation of Methods

Fructanase Mixture (powder)Fructanase Mixture (powder)

Documents
Certificate of Analysis
Select COA based on lot# 180401 120801 120901b 120901a 120901

Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Data Sheet

Related products
Similar Products
E-FRMXLQ – Fructanase Mixture (liquid) E-EXOIAN – exo-Inulinase (Aspergillus niger) E-ENDOIAN – endo-Inulinase (Aspergillus niger)

Related Carbohydrates
P-INUL – Inulin P-FOS28 – Fructooligosaccharides (FOS) O-KTR – 1-Kestose O-KTE – 1,1-Kestotetraose O-KPE – 1,1,1-Kestopentaose

Colourimetric Substrates
S-AZFR5 – Azo-Fructan S-AZFRXOI – Azo-Fructan

Associated Enzymes
E-INVPD-2G – Invertase E-ENLEV – endo-Levanase (Bacteroides thetaiotaomicron) E-SUCR – Sucrase (from yeast) E-INVRT – Invertase (fructofuranosidase) (yeast)

Safety Information
Symbol : GHS08
Signal Word : Danger
Hazard Statements : H334
Precautionary Statements : P261, P284, P304+P340, P342+P311, P501
Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Fructanase Mixture (powder)

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