Resistant Starch Assay Kit (Rapid)-Megazyme试剂盒

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Product code: K-RAPRS
€248.00

100 assays per kit

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Description Documents (40) Related Products Publications (7) Safety Information

Content: 100 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Resistant Starch
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 510
Signal Response: Increase
Linear Range: 4 to 100 mg of D-glucose per assay
Limit of Detection: 0.036 g/100 g
Reaction Time (min): ~ 5 h
Application examples: Plant materials, starch samples and other materials.
Method recognition: An updated modification of: AACC Method 32-40.01, AOAC Method 2002.02 and CODEX Method Type II

The Resistant Starch Assay Kit (Rapid) method is suitable for the analysis of resistant starch in pure starch, cereal and legume seeds and food samples.

This method is an update of the method of McCleary et al1 (AOAC Method 2002.02, AACC Method 32-40.01) employing incubation conditions similar to those used in AOAC Method 2017.16 for dietary fiber. The enzyme mixture employed [pancreatic α-amylase (PAA) and amyloglucosidase (AMG)] are those used by Englyst et al.2 except that both enzymes have been purified, standardised and stabilised. Digestion is performed using saturating levels of PAA and AMG with stirring at pH 6 and 37oC for 4 h, to simulate in vivo conditions in the human small intestine. Recent studies on the hydrolysis of “newer” resistant starch materials such as phosphate crosslinked starch (RS4) indicated that these incubation conditions are an essential requirement to obtain meaningful physiologically relevant values for RS.

The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (Megazyme method K-RINTDF). This method is physiologically based and designed to service the definition of DF announced by Codex Alimentarius in 2009.

1. McCleary, B. V., McNally, M. & Rossiter, P. (2002).  Measurement of Resistant Starch by Enzymic Digestion in Starch and Selected Plant Materials – Collaborative Study.  J. AOAC Int., 85, 1103-1111.

2. Englyst, H. N., Kingman, S. M. & Cummings, J. H. (1992).  Classification and measurement of nutritionally important starch fractions.  Eur. J. Clin. Nutr., 46 (Suppl. 2), S33-S50.

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Resistant Starch Assay Kit (Rapid)

Validation of Methods

Documents
Certificate of Analysis
Select COA based on lot# 211212 211112 211111 211110 210901

Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

FAQs Assay Protocol Data Calculator Product Performance Validation Report

Related products
Similar Products
K-RSTAR – Resistant Starch Assay Kit K-TSTA-100A – Total Starch Assay Kit (AA/AMG) K-DSTRS – Digestible and Resistant Starch Assay Kit K-TSHK – Total Starch HK Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-AVCHO – Available Carbohydrates Assay Kit

Related Carbohydrates
P-AMYL – Amylose (potato) P-BLDX-10G – β-Limit Dextrin O-MAL3 – Maltotriose O-MAL4 – Maltotetraose O-MAL5 – Maltopentaose O-MAL6 – Maltohexaose O-MAL7 – Maltoheptaose O-MAL8 – Maltooctaose

Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)

Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H314, H315, H319, H334
Precautionary Statements : P260, P261, P264, P280, P284, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340, P501
Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Resistant Starch Assay Kit (Rapid)

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