100 assays (manual) / 400 assays (auto-analyser)
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Content: | 100 assays (manual) / 400 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Phosphate |
Assay Format: | Spectrophotometer, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 360 |
Signal Response: | Increase |
Linear Range: | 0.1 to 10 μg of phosphate per assay |
Limit of Detection: | 0.16 mg/L |
Reaction Time (min): | ~ 20 min |
Application examples: | Processed foods and drinks, bakery products, dairy products, waste water samples, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, etc.). |
The Phosphate Assay Kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of phosphate in water, foodstuffs, beverages and other materials. The phosphate detection reaction employs the defined chemical substrate 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG). In the presence of phosphate, the substrate is converted enzymatically by purine nucleoside phosphorylase (PNPase) to the products ribose 1-phosphate and 2-amino-6-mercapto-7-methylpurine. An application note describes the dephosphorylation reaction of phytic acid using phytase and alkaline phosphatase (sold separately), and subsequent phosphate detection using the reagents provided in K-PHOS.
This method is suitable for analysis using spectrophotometer and auto-analyser.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.
Need other products? See our complete list of assay kits.
- Chemically defined detection method
- All reagents stable for > 2 years after preparation
- Rapid reaction (~ 20 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual and auto-analyser formats
Assay Protocol Data Calculator
Symbol : | Not Applicable |
Signal Word : | Not Applicable |
Hazard Statements : | Not Applicable |
Precautionary Statements : | Not Applicable |
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