Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)-Megazyme试剂盒

Product code: K-PullG6
€281.00

100/200 assays per kit

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Description Documents (41) Related Products Publications (13) Safety Information

Content: 100 / 200 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Pullulanase/Limit-Dextrinase
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: 0.18 U/mL for pullulanase preparations (50-fold dilution)
0.01 U/g for limit dextrinase in milled malt
Reproducibility (%): ~ 3%
Total Assay Time: ~ 10 min (Pullanase),
~ 30 min (Limit-Dextrinase)
Application examples: Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts.
Method recognition: Novel method

PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.

Explore more of our assay kit products for enzyme activity measurement.

Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)

Advantages
  • High sensitivity 
  • Suitable for manual and auto-analyser formats 
  • No transglycosylation interference 
  • Very cost effective 
  • All reagents stable for > 1 year after preparation 
  • Very specific
  • Simple format
  • Standard included

Documents
Certificate of Analysis
Select COA based on lot# 210823 210822 200812 200811 200313

Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Assay Protocols
Assay Protocols K-PullG6_DEXTRINASE_D-CHEMT K-PullG6_PULLULANASE_D-CHEMT

FAQs Assay Protocol Data Calculator Validation Report

Related products
Related Carbohydrates
P-PULLN – Pullulan P-BLDX-10G – β-Limit Dextrin O-MTMT – 63-α-D-Maltotriosyl-maltotriose O-GMH – 63-α-D-Glucosyl-maltotriosyl-maltotriose

Colourimetric Substrates
T-LDZ-200T – Limit-Dextrizyme Tablets I-AZPUL – AZCL-Pullulan

Associated Enzymes
E-PULKP – Pullulanase M1 (Klebsiella planticola) E-PULBL – Pullulanase M2 (Bacillus licheniformis)

Related Products
D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer) K-AMYLSD – α-Amylase SD Assay Kit K-BETA3 – β-Amylase Assay Kit (Betamyl-3) R-AMGR3 – Amyloglucosidase Assay Reagent O-PNPAG – 4-Nitrophenyl-α-D-glucopyranoside

Safety Information
Symbol : GHS07, GHS08
Signal Word : Danger
Hazard Statements : H315, H319, H334
Precautionary Statements : P261, P264, P280, P284, P302+P352, P304+P340, P305+P351+P338, P321, P332+P313, P342+P311, P501
Safety Data Sheet
Select SDS based on region GHS-US GHS-AU-BIOS GHS-AU-DELTA GHS-CN GHS-NZ SDS-EN SDS-BG SDS-CZ SDS-DE SDS-DK SDS-EE SDS-ES SDS-FI SDS-FR SDS-GR SDS-HR SDS-HU SDS-IE SDS-IS SDS-IT SDS-LT SDS-LV SDS-MT SDS-NL SDS-PL SDS-PT SDS-RO SDS-SE SDS-SI SDS-SK

Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method)

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