100/200 assays per kit
Prices exclude VAT
Available for shipping
North American customers click here
Content: | 100 / 200 assays per kit |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | Pullulanase/Limit-Dextrinase |
Assay Format: | Spectrophotometer |
Detection Method: | Absorbance |
Wavelength (nm): | 400 |
Signal Response: | Increase |
Limit of Detection: | 0.18 U/mL for pullulanase preparations (50-fold dilution) 0.01 U/g for limit dextrinase in milled malt |
Reproducibility (%): | ~ 3% |
Total Assay Time: | ~ 10 min (Pullanase), ~ 30 min (Limit-Dextrinase) |
Application examples: | Assay of microbial pullulanase preparations. Measurement of limit-dextrinase in malt extracts. |
Method recognition: | Novel method |
PullG6 assay for the measurement of pullulanase employs a water soluble defined substrate, namely 4,6-O-benzylidene-4-nitrophenyl-63-α-D-maltotriosyl-maltotriose (BPNPG3G3), coupled with the ancillary enzymes α-glucosidase and β-glucosidase. Upon hydrolysis of the substrate at the 1,6-α-linkage by pullulanase or limit-dextrinase, the released 4-nitrophenyl-β-maltotrioside is immediately hydrolysed to glucose and 4-nitrophenol by the concerted action of the α-glucosidase and β-glucosidase enzymes in the reagent mixture. The reaction is terminated and phenolate ions are developed by addition of dilute alkali. The absorbance is read at 400 nm and the value obtained correlates directly with pullulanase activity.
Explore more of our assay kit products for enzyme activity measurement.
- High sensitivity
- Suitable for manual and auto-analyser formats
- No transglycosylation interference
- Very cost effective
- All reagents stable for > 1 year after preparation
- Very specific
- Simple format
- Standard included
FAQs Assay Protocol Data Calculator Validation Report
Symbol : | GHS07, GHS08 |
Signal Word : | Danger |
Hazard Statements : | H315, H319, H334 |
Precautionary Statements : | P261, P264, P280, P284, P302+P352, P304+P340, P305+P351+P338, P321, P332+P313, P342+P311, P501 |
Megazyme产品应用分类:
食品检测 饲料检测 发酵业检测 红酒业检测 酿造业检测 奶制品检测 生物燃料 食物育种 实验室分析 研究
如果需要咨询Megazyme试剂盒说明书等其他技术问题可以访问Megazyme官网或者联系我们。