Hydrogen Peroxide Assay Kit (Megaplex Red)-Megazyme试剂盒

Hydrogen Peroxide Assay Kit (Megaplex Red)-Megazyme试剂盒
过氧化氢检测试剂盒(Megaplex Red)
英文名: Hydrogen Peroxide Assay Kit (Megaplex Red)
货号:K-MRH2O2
规格:1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
品牌: Megazyme
Product code: K-MRH2O2
€265.00

1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)

Prices exclude VAT

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Description Documents (33) Related Products Safety Information
Content: 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Hydrogen Peroxide
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance, Fluorescence
Wavelength (nm): (Fluorescence) 530-560 (ex), ~ 590 (em)
(Absorbance) 570
Signal Response: Increase
Linear Range: (Fluorescence) 0.0034-0.17 µg of H2O2 per assay (0.1-5 µM in-assay)
(Absorbance) 0.034-0.68 µg of H2O2 per assay (1-20 µM in-assay)
Limit of Detection: (Fluorescence) 0.0034 ug/mL
(Absorbance)
 0.25 ug/mL
Reproducibility (%): (Fluorescence) ~ 8%
(Absorbance) ~ 8%
Reaction Time (min): (Fluorescence) 30 min
(Absorbance)
 
15 min
Application examples: Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).

内容:1000 次检测(微孔板)/100 次检测(手动)/400 次检测(自动分析仪)
运输温度:环境
储存温度:短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性:在推荐的储存条件下 > 2 年
分析物:过氧化氢
检测形式:分光光度计、微孔板、自动分析仪
检测方法:吸光度、荧光
波长 (nm):(荧光)530-560 (ex), ~ 590 (em)
(吸光度)570
信号响应:增加
线性范围:(荧光)每次测定 0.0034-0.17 µg H2O2(测定中 0.1-5 µM)
(吸光度)每次测定 0.034-0.68 µg H2O2(测定中 1-20 µM)
检测限:(荧光)0.0034 ug/mL
(吸光度)0.25 ug/mL
重现性 (%): (荧光) ~ 8%
(吸光度)~ 8%
反应时间(分钟):(荧光)30 分钟
(吸光度)15 分钟
应用示例:食品和饮料样品,例如牛奶或奶制品、葡萄酒、果汁、纸张(和纸板)、废物处理样品和其他材料(例如生物培养物等)。

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.

Display all assay test kits available.

过氧化氢检测试剂盒为过氧化氢的测量提供了一种简单可靠的方法。 该试剂盒使用高度灵敏的 Megaplex Red 探针,在辣根过氧化物酶 (HRP) 存在的情况下,可以测量样品中的 H2O2。 通过 HRP 氧化探针后,形成有色产物(试卤灵),可以使用 530-560 nm 范围内的激发和约 590 nm 的发射或 570 nm 的比色法在荧光模式下测量该产物。 Megaplex Red 的激发和发射最大值分别为 571 nm 和 585 nm。

显示所有可用的化验试剂盒。

Fluorometric/UV method for the determination of H2O2 content in a variety of samples.

Hydrogen Peroxide Assay Kit (Megaplex Red)

Advantages
  • Simple format
  • Very competitive price (cost per test)
  • Ability to run in fluorescence or absorbance mode
  • User friendly – Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Suitable for manual, microplate and auto-analyser formats
  • All reagents stable for > 2 years
Documents

Certificate of Analysis

Safety Data Sheet

Assay Protocol Data Calculator

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Safety Information
Symbol : GHS06
Signal Word : Danger
Hazard Statements : H301
Precautionary Statements : P264, P270, P301+P310, P321, P330
Safety Data Sheet
Hydrogen Peroxide Assay Kit (Megaplex Red)

Megazyme产品应用分类:
食品检测 饲料检测 发酵业检测 红酒业检测 酿造业检测 奶制品检测 生物燃料 食物育种 实验室分析 研究
如果需要咨询Megazyme试剂盒说明书等其他技术问题可以访问Megazyme官网或者联系我们。