Rapid Integrated Total Dietary Fiber Assay Kit-Megazyme试剂盒

Rapid Integrated Total Dietary Fiber Assay Kit-Megazyme试剂盒
英文名:Rapid Integrated Total Dietary Fiber Assay Kit
规格:100 assays per kit
品牌: Megazyme
Product code: K-RINTDF

100 assays per kit

Prices exclude VAT

Available for shipping

Description Documents (38) Related Products Publications (8) Safety Information
Content: 100 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Dietary Fiber
Assay Format: Enzymatic
Detection Method: Gravimetric/HPLC
Signal Response: Increase
Limit of Detection: 0.5 g/100 g
Total Assay Time: ~ 3 h work (over 1-2 days)
Application examples: Food ingredients, food products and other materials.
Method recognition: AACC Method 32-60.01, AOAC Method 2017.16, ICC Standard Method No. 185 and CODEX Method Type I

内容:每个试剂盒 100 次检测
稳定性:在推荐的储存条件下 > 2 年
检测限:0.5 g/100 g
总检测时间:~ 3 小时工作(超过 1-2 天)
方法识别:AACC Method 32-60.01、AOAC Method 2017.16、ICC Standard Method No. 185 和 CODEX Method Type I

The Rapid Integrated Total Dietary Fiber Assay Kit method is validated under collaborative study (AACC Method 32-60.01, AOAC Method 2017.16, ICC Standard No. 185) and is recognized as a Type I Method by CODEX Alimentarius. The K-RINTDF method is the recommended one for the measurement of total dietary fiber in all foods that may or may not contain resistant starch. This method is updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time. Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS4). Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25) are no longer produced.

In this improved, rapid method, the incubation time with PAA + AMG is reduced to 4 h and the levels of both PAA and AMG are increased to ensure that resistant starch levels obtained with a set of control samples are consistent with ileostomy data. Under these conditions, the DF values obtained for most samples are the same as those obtained with AOAC Methods 2009.01 and 2011.25.

The dietary fiber fractions that are measured with this method are:

1. High Molecular Weight Dietary Fiber (HMWDF) including Insoluble Dietary Fiber (IDF) and High Molecular Weight Soluble Dietary Fiber (SDFP; soluble dietary fiber which is precipitated in the presence of 78% aqueous ethanol), and

2. Low Molecular Weight Soluble Dietary Fiber (SDFS; water soluble dietary fiber that is soluble in the presence of 78% aqueous ethanol).

Alternatively, IDF, SDFP and SDFS can be measured separately.

The enzymes used in this method are high purity and effectively devoid of contaminating enzymes active on other dietary fiber components such as β-glucan, pectin and arabinoxylan. They are supplied as freeze-dried powders; allowing the use of glycerol as an internal standard in the method.

See our full range of dietary fiber assay kits.

快速综合总膳食纤维检测试剂盒方法在合作研究(AACC 方法 32-60.01、AOAC 方法 2017.16、ICC 标准第 185 号)下得到验证,并被食品法典委员会认定为 I 类方法。 K-RINTDF 方法被推荐用于测量所有可能含有或不含抗性淀粉的食物中的总膳食纤维。此方法已更新,以更符合人体小肠的体内条件,即 4 小时的孵育时间。在这些条件下,可以更准确地测量抗性淀粉,包括磷酸交联淀粉 (RS4)。使用更高的酶浓度可确保不再生产在综合总膳食纤维程序(AOAC 方法 2009.01 和 2011.25)的孵育条件下由非抗性淀粉生产的抗性麦芽糊精。

在这种改进的快速方法中,PAA + AMG 的孵育时间减少到 4 小时,PAA 和 AMG 的水平都增加了,以确保用一组对照样品获得的抗性淀粉水平与回肠造口术数据一致。在这些条件下,大多数样品获得的 DF 值与使用 AOAC 方法 2009.01 和 2011.25 获得的值相同。


1. 高分子量膳食纤维 (HMWDF),包括不溶性膳食纤维 (IDF) 和高分子量可溶性膳食纤维 (SDFP;在 78% 乙醇水溶液中沉淀的可溶性膳食纤维),以及

2. 低分子量可溶性膳食纤维(SDFS;水溶性膳食纤维,在 78% 含水乙醇存在下可溶)。

或者,可以分别测量 IDF、SDFP 和 SDFS。

该方法中使用的酶纯度高,有效地避免了对其他膳食纤维成分(如 β-葡聚糖、果胶和阿拉伯木聚糖)具有活性的污染酶。它们以冷冻干燥粉末的形式提供;允许在该方法中使用甘油作为内标。


View Dietary Fiber Measurement Guide – Which Method for which sample?
* See McCleary, B. V., Sloane, N & Draga, A. (2015). Determination of total dietary fibre and available carbohydrates: a rapid integrated procedure that simulates in vivo digestion. Starch/Starke, 66, 1-24.

查看膳食纤维测量指南 – 哪种方法适用于哪种样品?

* 参见 McCleary, B. V., Sloane, N & Draga, A. (2015)。 总膳食纤维和可用碳水化合物的测定:模拟体内消化的快速综合程序。 淀粉/斯塔克,66,1-24。

Rapid Integrated Total Dietary Fiber Assay Kit
Validation of Methods
  • More rapid measurement – incubation time with PAA + AMG reduced to 4 h in comparison with AOAC 2009.01 (increased levels of enzyme employed)
  • DF values for most samples are very similar to those obtained with AOAC Method 2009.01
  • Rapid Integrated Total Dietary Fiber method removes all of the limitations that have been identified with AOAC Method 2009.01*
  • All reagents stable for > 2 years after preparation
  • The method is consistent with the CODEX Alimentarius definition of dietary fiber
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Very competitive price (cost per test)
Certificate of Analysis
Select COA based on lot# 211134 210732 210715 210633 210338
Safety Data Sheet

FAQs Assay Protocol Data Calculator

Related products
Similar Products
K-INTDF – Integrated Total Dietary Fiber Assay Kit K-TDFR-200A – Total Dietary Fiber Assay Kit K-TDFC – Total Dietary Fiber Controls K-AVCHO – Available Carbohydrates Assay Kit
Related Carbohydrates
P-BGBM – β-Glucan (Barley; Medium Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-INUL – Inulin P-FOS28 – Fructooligosaccharides (FOS) P-XANTH – Xanthan Gum
Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-BSPRT-40ML – Protease (Subtilisin A from Bacillus licheniformis) E-BGOG – Oligo-α-1,6-Glucosidase plus β-Galactosidase
Related Products
D-TDFBTH – Water Bath for Total Dietary Fiber Procedures G-AMBOH – Amberlite FPA OH Ion Exchange Resin G-AMBH – Ambersep 200 H+ Ion Exchange Resin G-CEL-500G – Celite K-RSTAR – Resistant Starch Assay Kit


K-INTDF – 综合总膳食纤维检测试剂盒 K-TDFR-200A – 总膳食纤维检测试剂盒 K-TDFC – 总膳食纤维对照 K-AVCHO – 可用碳水化合物检测试剂盒
P-BGBM – β-葡聚糖(大麦;中等粘度) P-WAXYM – 阿拉伯木聚糖(小麦粉;中等粘度) P-BGOM – β-葡聚糖(燕麦;中等粘度) P-INUL – 菊粉 P-FOS28 – 低聚果糖 (FOS ) P-XANTH – 黄原胶
E-AMGDF-40ML – 淀粉葡糖苷酶(黑曲霉) E-BSPRT-40ML – 蛋白酶(来自地衣芽孢杆菌的枯草杆菌蛋白酶 A) E-BGOG – Oligo-α-1,6-Glucosidase plus β-Galactosidase
D-TDFBTH – 用于总膳食纤维程序的水浴 G-AMBOH – Amberlite FPA OH- 离子交换树脂 G-AMBH – Ambersep 200 H+ 离子交换树脂 G-CEL-500G – 硅藻土 K-RSTAR – 抗性淀粉测定试剂盒

Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H318, H334
Precautionary Statements : P261, P280, P284, P304+P340, P305+P351+P338, P310, P342+P311, P501
Safety Data Sheet
Rapid Integrated Total Dietary Fiber Assay Kit

食品检测 饲料检测 发酵业检测 红酒业检测 酿造业检测 奶制品检测 生物燃料 食物育种 实验室分析 研究