Total Dietary Fiber Assay Kit-Megazyme试剂盒

Total Dietary Fiber Assay Kit-Megazyme试剂盒
英文名:Total Dietary Fibre Assay Kit
规格:200 assays per kit
品牌: Megazyme
Product code: K-TDFR-200A


200 assays 100 assays

200 assays

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Description Documents (44) Related Products Publications (20) Safety Information
Content: 100 assays / 200 assays
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Dietary Fiber
Assay Format: Enzymatic
Detection Method: Gravimetric
Signal Response: Increase
Limit of Detection: 0.5 g/100 g
Total Assay Time: ~ 100 min
Application examples: Food ingredients, food products and other materials.
Method recognition: AACC Method 32-05.01, AACC Method 32-06.01, AACC Method 32-07.01, AACC Method 32-21.01, AOAC Method 985.29, AOAC Method 991.42, AOAC Method 991.43, AOAC Method 993.19, CODEX Method Type I and GB Standard 5009.88-2014

内容:100 次检测 / 200 次检测
稳定性:在推荐的储存条件下 > 2 年
检测限:0.5 g/100 g
总测定时间:~ 100 分钟
方法识别:AACC Method 32-05.01、AACC Method 32-06.01、AACC Method 32-07.01、AACC Method 32-21.01、AOAC Method 985.29、AOAC Method 991.42、AOAC Method 991.43、AOAC Method 993.19、CODEX Method Type I和GB Standard 5009.88-2014

The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods.

View Dietary Fiber Measurement Guide – Which Method for which sample?

Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.

Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.

Two separate methods are described in the associated assay protocol:

Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

Based on AACC method 32-05.01 and AOAC Method 985.29.

总膳食纤维检测试剂盒,用于根据 AOAC 和 AACC 批准的方法分析总膳食纤维、可溶性和不溶性膳食纤维。

查看膳食纤维测量指南 – 哪种方法适用于哪种样品?


膳食纤维是复杂有机物质的混合物,包括亲水性化合物,如可溶性和不溶性多糖和不可消化的寡糖,以及一系列不可溶胀、或多或少疏水的化合物,如角质、木栓质和木质素。我们的测定方案中概述的总膳食纤维的测定和分析程序基于 Lee 等人 1 和 Prosky 等人 2,3 的方法(AOAC 991.43、AOAC 985.29、AACC 32-07.01 和 AACC 32 -05.01)。然而,Megazyme Total Dietary Fiber Kit 中的酶也可用于其他膳食纤维分析方法,例如 AACC 方法 32-21.01 和 AACC 方法 32-06.01。

1. 官方分析化学家协会。 (1985 年)。官方分析方法,第 14 版,第 1 次增补。秒。 43,A14-43,A20,第 399 页。
2. 官方分析化学家协会。 (1986 年)。方法的变化。 J.副教授。离开。肛门。化学,69,370。
3. 官方分析化学家协会。 (1987 年)。方法的变化。 J.副教授。离开。肛门。化学,70,393。

见总裁判报告:AOAC INTERNATIONAL 杂志,卷。 81 号,1998 年第 1 期。


方法 1:总、可溶性和不溶性膳食纤维的测定
基于 AOAC 方法 991.43“食品中的总、可溶性和不溶性膳食纤维”(1991 年第一行动)和 AACC 方法 32-07.01“食品和食品中可溶性、不溶性和总膳食纤维的测定”(最终批准 10- 16-91)。

方法 2:总膳食纤维的测定
基于 AACC 方法 32-05.01 和 AOAC 方法 985.29。

Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.

Total Dietary Fiber Assay Kit

See our full range of starch and dietary fiber products.

Validation of Methods
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years
  • High purity / standardised enzymes employed
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format
Certificate of Analysis
Select COA based on lot# 200A 210427 200A 210334 200A 210333 200A 201112 200A 190505 100A 210909 100A 200104 100A 190302 100A 180411 100A 180405
Safety Data Sheet

FAQs Assay Protocol Data Calculator Letter of Endorsement

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K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-INTDF – Integrated Total Dietary Fiber Assay Kit K-TDFC – Total Dietary Fiber Controls K-ACHDF – Available Carbohydrates/Dietary Fiber Assay Kit
Related Carbohydrates
P-BGBM – β-Glucan (Barley; Medium Viscosity) P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-INUL – Inulin P-FOS28 – Fructooligosaccharides (FOS) P-XANTH – Xanthan Gum
Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-BLAAM-40ML – α-Amylase (Bacillus licheniformis) E-BSPRT-40ML – Protease (Subtilisin A from Bacillus licheniformis)
Related Products
D-TDFBTH – Water Bath for Total Dietary Fiber Procedures G-CEL-500G – Celite G-AMBH – Ambersep 200 H+ Ion Exchange Resin G-AMBOH – Amberlite FPA OH Ion Exchange Resin

常见问题 检测协议数据计算器 认可信

K-RINTDF – 快速综合总膳食纤维检测试剂盒 K-INTDF – 综合总膳食纤维检测试剂盒 K-TDFC – 总膳食纤维对照 K-ACHDF – 可用碳水化合物/膳食纤维检测试剂盒
P-BGBM – β-葡聚糖(大麦;中等粘度) P-WAXYM – 阿拉伯木聚糖(小麦粉;中等粘度) P-BGOM – β-葡聚糖(燕麦;中等粘度) P-INUL – 菊粉 P-FOS28 – 低聚果糖 (FOS ) P-XANTH – 黄原胶
E-AMGDF-40ML – 淀粉葡萄糖苷酶(黑曲霉) E-BLAAM-40ML – α-淀粉酶(地衣芽孢杆菌) E-BSPRT-40ML – 蛋白酶(地衣芽孢杆菌的枯草杆菌蛋白酶 A)
D-TDFBTH – 用于全膳食纤维程序的水浴 G-CEL-500G – 硅藻土 G-AMBH – Ambersep 200 H+ 离子交换树脂 G-AMBOH – Amberlite FPA OH- 离子交换树脂

Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H318, H334
Precautionary Statements : P261, P280, P284, P304+P340, P305+P351+P338, P310, P342+P311, P501
Safety Data Sheet
Total Dietary Fiber Assay Kit

食品检测 饲料检测 发酵业检测 红酒业检测 酿造业检测 奶制品检测 生物燃料 食物育种 实验室分析 研究