Cellulase Assay Kit (CellG5 Method)-Megazyme试剂盒

Cellulase Assay Kit (CellG5 Method)-Megazyme试剂盒
内切纤维素酶检测试剂盒(CELLG5方法)
英文名:Cellulase Assay Kit (CELLG5 Method)
货号:K-CELLG5-2V
规格:60 / 120 assays (manual) / 240 (auto-analyser)
品牌: Megazyme
Product code: K-CellG5-2V

Content:

120 / 240 assays (manual) / 480 assays (auto-analyser) 60 / 120 assays (manual) / 240 assays (auto-analyser)
€220.00

60 / 120 assays (manual) / 240 assays (auto-analyser)

Prices exclude VAT

Available for shipping

Description Documents (45) Related Products Publications (8) Safety Information
Content: (K-CellG5-2V)
60 / 120 assays (manual) / 240 assays (auto-analyser) or
(K-CellG5-4V)

120 / 240 assays (manual) / 480 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: endo-Cellulase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: 1.2 x 10-3 U/mL
Reproducibility (%): ~ 3%
Total Assay Time: 10 min
Application examples: Fermentation broths, industrial enzyme preparations and biofuels research.
Method recognition: Novel method

内容:(K-CellG5-2V)
60 / 120 次检测(手动)/240 次检测(自动分析仪)或
(K-CellG5-4V)
120 / 240 次检测(手动)/480 次检测(自动分析仪)
运输温度:环境
储存温度:短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性:在推荐的储存条件下 > 2 年
分析物:内切纤维素酶
检测形式:分光光度计、自动分析仪
检测方法:吸光度
波长(nm):400
信号响应:增加
检测限:1.2 x 10-3 U/mL
再现性(%):~ 3%
总检测时间:10 分钟
应用示例:发酵液、工业酶制剂和生物燃料研究。
方法识别:新方法

Cellulase Activity Assay Kit.

The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).

The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

纤维素酶活性测定试剂盒。

用于测定内切纤维素酶(endo-1,4-β-glucanase)的 CellG5 检测试剂包含两种成分; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) 和 2) 热稳定的 β-葡萄糖苷酶。 酮封闭基团阻止 β-葡萄糖苷酶对 BPNPG5 的任何水解作用。 与内切纤维素酶一起孵育会产生一种非封闭的比色寡糖,该寡糖会被辅助 β-葡萄糖苷酶快速水解。 因此,4-硝基苯酚的形成速度与内切纤维素酶对 BPNPG5 的水解直接相关。 反应终止,加入 Tris 缓冲溶液 (pH 9.0) 后酚盐颜色显色。

CellG5 测定法代表了纤维素酶测量方法的巨大进步,该方法传统上依赖于基质,如 CM 纤维素、Avicel、纤维寡糖、滤纸或染色多糖,包括 CMC 刚果红或纤维素天蓝色。

View Cellulase Activity Assay Protocol.

View our complete list of assay kits for enzyme activities.

Cellulase Assay Kit (CellG5 Method)

Advantages
  • Very cost effective
  • All reagents stable for > 4 years
  • Completely specific for cellulase (endo-1,4-glucanase)
  • Generally applicable and highly sensitive
  • Simple format. Well suited to automation
  • Standard included
Documents
Certificate of Analysis
Select COA based on lot# 4V 210305 4V 200813 4V 200337 4V 191202 4V 191002 2V 210304 2V 200902 2V 200338 2V 191203 2V 191001
Safety Data Sheet
Assay Protocols
Assay Protocols K-CellG5-2V_D-CHEM2910 K-CellG5-2V_D-CHEMT

Assay Protocol Data Calculator Validation Report

Related products
Similar Products
K-CellG3 – Cellulase Assay Kit (CellG3 Method) T-CCZ-200T – Cellazyme C Tablets S-ACMCL – Azo-CM-Cellulose (Liquid)
Related Carbohydrates
P-CMC4M – Carboxymethyl Cellulose 4M P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGOH – β-Glucan (Oat; High Viscosity) P-BGCFA – β-Glucan CFA Standard P-GLCML – Glucomannan (Konjac; Low Viscosity) P-GLCMH – Glucomannan (Konjac; High Viscosity) P-XYGLN – Xyloglucan (Tamarind) O-CTR-50MG – Cellotriose O-CTE-50MG – Cellotetraose O-CPE-20MG – Cellopentaose O-CHE – Cellohexaose
Associated Enzymes
E-CELAN – Cellulase (endo-1,4-β-D-glucanase)
(Aspergillus niger) E-CELBA – Cellulase (endo-1,4-β-D-glucanase)
(Bacillus amyloliquefaciens) E-CELTE – Cellulase (endo-1,4-β-D-glucanase)
(Talaromyces emersonii) E-CELTH – Cellulase (endo-1,4-β-D-glucanase)
(Thermobifida halotolerans) E-CELTR – Cellulase (endo-1,4-β-D-glucanase)
(Trichoderma longibrachiatum) E-CELTM – Cellulase (endo-1,4-β-D-glucanase)
(Thermotoga maritima)

检测方案
检测方案 K-CellG5-2V_D-CHEM2910 K-CellG5-2V_D-CHEMT

分析方案数据计算器验证报告

相关产品
类似产品
K-CellG3 – 纤维素酶检测试剂盒(CellG3 方法) T-CCZ-200T – Cellazyme C 片剂 S-ACMCL – 偶氮-CM-纤维素(液体)
相关碳水化合物
P-CMC4M – 羧甲基纤维素 4M P-BGBL – β-葡聚糖(大麦;低粘度) P-BGBM – β-葡聚糖(大麦;中等粘度) P-BGBH – β-葡聚糖(大麦;高粘度) P-BGOM – β-葡聚糖(燕麦;中等粘度) P-BGOH – β-葡聚糖(燕麦;高粘度) P-BGCFA – β-葡聚糖 CFA 标准品 P-GLCML – 葡甘聚糖(魔芋;低粘度) P-GLCMH – 葡甘聚糖(魔芋;高粘度)P-XYGLN – 木葡聚糖(罗望子) O-CTR-50MG – 纤维三糖 O-CTE-50MG – 纤维四糖 O-CPE-20MG – 纤维五糖 O-CHE – 纤维六糖
相关酶
E-CELAN – 纤维素酶(endo-1,4-β-D-glucanase)
(Aspergillus niger) E-CELBA – 纤维素酶 (endo-1,4-β-D-glucanase)
(解淀粉芽孢杆菌) E-CELTE – 纤维素酶 (endo-1,4-β-D-glucanase)
(Talaromyces emersonii) E-CELTH – 纤维素酶 (endo-1,4-β-D-glucanase)
(Thermobifida halotolerans) E-CELTR – 纤维素酶 (endo-1,4-β-D-glucanase)
(Trichoderma longibrachiatum) E-CELTM – 纤维素酶 (endo-1,4-β-D-glucanase)
(Thermotoga maritima)

Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Cellulase Assay Kit (CellG5 Method)

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