英文名:Cellulase Assay Kit (CELLG5 Method)
货号:K-CELLG5-2V
规格:60 / 120 assays (manual) / 240 (auto-analyser)
品牌: Megazyme
Content:
60 / 120 assays (manual) / 240 assays (auto-analyser)
Prices exclude VAT
Available for shipping
Content: | (K-CellG5-2V) 60 / 120 assays (manual) / 240 assays (auto-analyser) or (K-CellG5-4V) 120 / 240 assays (manual) / 480 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | endo-Cellulase |
Assay Format: | Spectrophotometer, Auto-analyser |
Detection Method: | Absorbance |
Wavelength (nm): | 400 |
Signal Response: | Increase |
Limit of Detection: | 1.2 x 10-3 U/mL |
Reproducibility (%): | ~ 3% |
Total Assay Time: | 10 min |
Application examples: | Fermentation broths, industrial enzyme preparations and biofuels research. |
Method recognition: | Novel method |
内容:(K-CellG5-2V)
60 / 120 次检测(手动)/240 次检测(自动分析仪)或
(K-CellG5-4V)
120 / 240 次检测(手动)/480 次检测(自动分析仪)
运输温度:环境
储存温度:短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性:在推荐的储存条件下 > 2 年
分析物:内切纤维素酶
检测形式:分光光度计、自动分析仪
检测方法:吸光度
波长(nm):400
信号响应:增加
检测限:1.2 x 10-3 U/mL
再现性(%):~ 3%
总检测时间:10 分钟
应用示例:发酵液、工业酶制剂和生物燃料研究。
方法识别:新方法
Cellulase Activity Assay Kit.
The CellG5 assay reagent for the measurement of endo-cellulase (endo-1,4-β-glucanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the β-glucosidase on BPNPG5. Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
The CellG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.
纤维素酶活性测定试剂盒。
用于测定内切纤维素酶(endo-1,4-β-glucanase)的 CellG5 检测试剂包含两种成分; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) 和 2) 热稳定的 β-葡萄糖苷酶。 酮封闭基团阻止 β-葡萄糖苷酶对 BPNPG5 的任何水解作用。 与内切纤维素酶一起孵育会产生一种非封闭的比色寡糖,该寡糖会被辅助 β-葡萄糖苷酶快速水解。 因此,4-硝基苯酚的形成速度与内切纤维素酶对 BPNPG5 的水解直接相关。 反应终止,加入 Tris 缓冲溶液 (pH 9.0) 后酚盐颜色显色。
CellG5 测定法代表了纤维素酶测量方法的巨大进步,该方法传统上依赖于基质,如 CM 纤维素、Avicel、纤维寡糖、滤纸或染色多糖,包括 CMC 刚果红或纤维素天蓝色。
View Cellulase Activity Assay Protocol.
View our complete list of assay kits for enzyme activities.
- Very cost effective
- All reagents stable for > 4 years
- Completely specific for cellulase (endo-1,4-glucanase)
- Generally applicable and highly sensitive
- Simple format. Well suited to automation
- Standard included
Assay Protocol Data Calculator Validation Report
(Aspergillus niger) E-CELBA – Cellulase (endo-1,4-β-D-glucanase)
(Bacillus amyloliquefaciens) E-CELTE – Cellulase (endo-1,4-β-D-glucanase)
(Talaromyces emersonii) E-CELTH – Cellulase (endo-1,4-β-D-glucanase)
(Thermobifida halotolerans) E-CELTR – Cellulase (endo-1,4-β-D-glucanase)
(Trichoderma longibrachiatum) E-CELTM – Cellulase (endo-1,4-β-D-glucanase)
(Thermotoga maritima)
检测方案
检测方案 K-CellG5-2V_D-CHEM2910 K-CellG5-2V_D-CHEMT
分析方案数据计算器验证报告
相关产品
类似产品
K-CellG3 – 纤维素酶检测试剂盒(CellG3 方法) T-CCZ-200T – Cellazyme C 片剂 S-ACMCL – 偶氮-CM-纤维素(液体)
相关碳水化合物
P-CMC4M – 羧甲基纤维素 4M P-BGBL – β-葡聚糖(大麦;低粘度) P-BGBM – β-葡聚糖(大麦;中等粘度) P-BGBH – β-葡聚糖(大麦;高粘度) P-BGOM – β-葡聚糖(燕麦;中等粘度) P-BGOH – β-葡聚糖(燕麦;高粘度) P-BGCFA – β-葡聚糖 CFA 标准品 P-GLCML – 葡甘聚糖(魔芋;低粘度) P-GLCMH – 葡甘聚糖(魔芋;高粘度)P-XYGLN – 木葡聚糖(罗望子) O-CTR-50MG – 纤维三糖 O-CTE-50MG – 纤维四糖 O-CPE-20MG – 纤维五糖 O-CHE – 纤维六糖
相关酶
E-CELAN – 纤维素酶(endo-1,4-β-D-glucanase)
(Aspergillus niger) E-CELBA – 纤维素酶 (endo-1,4-β-D-glucanase)
(解淀粉芽孢杆菌) E-CELTE – 纤维素酶 (endo-1,4-β-D-glucanase)
(Talaromyces emersonii) E-CELTH – 纤维素酶 (endo-1,4-β-D-glucanase)
(Thermobifida halotolerans) E-CELTR – 纤维素酶 (endo-1,4-β-D-glucanase)
(Trichoderma longibrachiatum) E-CELTM – 纤维素酶 (endo-1,4-β-D-glucanase)
(Thermotoga maritima)
Symbol : | Not Applicable |
Signal Word : | Not Applicable |
Hazard Statements : | Not Applicable |
Precautionary Statements : | Not Applicable |
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