Available Carbohydrates Assay Kit-Megazyme试剂盒

Available Carbohydrates Assay Kit-Megazyme试剂盒
英文名:Available Carbohydrates Assay Kit
规格:100 Assays of each per kit
品牌: Megazyme
Product code: K-AVCHO

100 Assays of each per kit

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Description Documents (39) Related Products Publications (3) Safety Information
Content: 100 assays of each per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Available Carbohydrates, Dietary Fiber
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay
Limit of Detection: 1.475 g/100 g
Reaction Time (min): ~ 5 h
Application examples: Food ingredients, food products and other materials.
Method recognition: AOAC Method 2020.07

内容:每个试剂盒 100 次检测
稳定性:在推荐的储存条件下 > 2 年
线性范围:每次测定 4 至 80 μg D-葡萄糖、D-果糖或 D-半乳糖
检测限:1.475 g/100 g
反应时间(分钟):~ 5 小时
方法识别:AOAC Method 2020.07

The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving ‘First Action’ status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).

* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.

可用碳水化合物检测试剂盒方法适用于测定可用碳水化合物 (AVCHO),包括 *总可消化淀粉 (TDS) 加上麦芽糖糊精、蔗糖、D-葡萄糖、D-果糖和乳糖。接收“第一行动”状态的新改进方法:AOAC 2020.07。该方法旨在模拟人体小肠中的体内条件(即使用 PAA + AMG 孵育 4 小时),同时与膳食纤维 (DF) 方法学的最新进展(K-RINTDF:AOAC 方法 2017.16)并行,并根据Codex Alimentarius 于 2009 年宣布了新的(基于生理的)DF 定义。此外,蔗糖被一种特定的“蔗糖酶”水解,该酶(与传统上用于该反应的转化酶不同)对低聚果糖没有作用( FOS)。

* 总可消化淀粉 (TDS) 定义为在 4 小时内消化的淀粉,是可在人体小肠中消化和吸收的碳水化合物的一部分。

See our full range of dietary fiber assay kits.

Available Carbohydrates Assay Kit

Validation of Methods
Certificate of Analysis
Select COA based on lot# 211209 211128 211120 211119 210934
Safety Data Sheet

Assay Protocol Data Calculator Product Performance Validation Report

Related products
Similar Products
K-ACHDF – Available Carbohydrates/Dietary Fiber Assay Kit K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit K-DSTRS – Digestible and Resistant Starch Assay Kit K-RAPRS – Resistant Starch Assay Kit (Rapid) K-TDFC – Total Dietary Fiber Controls
Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger) E-BGLAN – β-Galactosidase (Aspergillus niger) E-SUCR – Sucrase (from yeast)
K-ACHDF – 可用碳水化合物/膳食纤维检测试剂盒 K-RINTDF – 快速综合总膳食纤维检测试剂盒 K-DSTRS – 可消化和抗性淀粉检测试剂盒 K-RAPRS – 抗性淀粉检测试剂盒(快速) K-TDFC – 总膳食纤维 控件
E-AMGDF-40ML – 淀粉葡萄糖苷酶(黑曲霉) E-BGLAN – β-半乳糖苷酶(黑曲霉) E-SUCR – 蔗糖酶(来自酵母)
Safety Information
Symbol : GHS05, GHS08
Signal Word : Danger
Hazard Statements : H314, H334, H360
Precautionary Statements : P201, P202, P260, P261, P264, P280, P284, P301+P330+P331, P304+P340, P342+P311, P501
Safety Data Sheet
Available Carbohydrates Assay Kit

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