Megazyme K-ASCO 维生素C[L-抗坏血酸]检测试剂盒中文说明书
维生素C[L-抗坏血酸]检测试剂盒 K-ASCO
英文名：L-Ascorbic Acid (L-Ascorbate)
货号：K-ASCO
规格：40 assays (manual)
品牌： Megazyme
For the specific assay of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products. Content:40 assays per kit
用于饮料，肉，面粉，乳制品和蔬菜产品中L-抗坏血酸的特异性测定。 内容：每个试剂盒40次检测
Colourimetric method for the determination of L-Ascorbic Acid
in foodstuffs, feed, wine and other materials
Principle:
(5-methylphenazinium methosulphate)
(1) L-Ascorbic acid + R-H2 + MTT → dehydroascorbate + MTT-formazan + H+
(ascorbic acid oxidase)
(2) L-Ascorbic acid + ½O2 → dehydroascorbate + H2O
Kit size: 40 assays (manual) / 400 (microplate)
/ 400 (auto-analyser)
Method: Spectrophotometric at 578 nm
Reaction time: ~ 8 min
Detection limit: 0.175 mg/L
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 6 months after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
| Content: | 40 assays (manual) / 400 assays (microplate) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Ascorbic Acid |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 578 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 30 µg of L-ascorbic acid per assay |
| Limit of Detection: | 0.175 mg/L |
| Reaction Time (min): | ~ 8 min |
| Application examples: | Wine, beer, fruit juices, soft drinks, jam, milk, dairy products (e.g. cheese), dietetic foods, baby foods, processed meat, baking additives, fruit and vegetables (e.g. tomato and potato), pharmaceuticals, feed and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by MEBAK |
The Ascorbic Acid (L-Ascorbate) assay kit is for the specific measurement and analysis of L-ascorbic acid in beverages, meat, flour, dairy and vegetable products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
| 内容： | 40项试验(手册)/400项试验(微板)/400项试验(自动分析仪) |
| 船运温度： | 环境 |
| 储存温度： | 短期稳定性：2-8oC， 长期稳定性：见单个组件标签 |
| 稳定性： | >2年在建议的储存条件下 |
| 分析物： | 抗坏血酸 |
| 化验格式： | 分光光度计，微板，自动分析仪 |
| 检测方法： | 吸光度 |
| 波长(纳米)： | 578 |
| 信号响应： | 增加 |
| 线性范围： | 0.5至30克L-抗坏血酸每次测定 |
| 检测极限： | 0.175毫克/升 |
| 反应时间(最低)： | ~8分钟 |
| 应用实例： | 葡萄酒、啤酒、果汁、软饮料、果酱、牛奶、乳制品(如奶酪)、饮食食品、婴儿食品、加工肉类、烘焙添加剂、水果和蔬菜(如西红柿和土豆)、药品、饲料和其他材料(例如生物培养物、样品等)。 |
| 方法识别： | 基于这一原则的方法已被MEBAK所接受。 |
抗坏血酸(L-抗坏血酸)测定试剂盒用于饮料、肉类、面粉、乳制品和蔬菜中L-抗坏血酸的测定和分析。
内容说明：如果将所有卷减少一半，每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).
