Megazyme K-ASPTM 天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒说明书

Megazyme K-ASPTM 天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒说明书

天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒
英文名:Aspartame Assay Kit
货号:K-ASPTM
规格:50 assays (manual)

Content: 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Aspartame
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Decrease
Linear Range: 10 to 150 µg of aspartame per assay
Limit of Detection: 0.57 mg/L
Reaction Time (min): ~ 5 min
Application examples: Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic products, jam, chocolate and other materials.
Method recognition: Novel method
内容: 50项试验(手册)/500项试验(微板)/500项试验(自动分析仪)
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 阿斯巴甜
化验格式: 分光光度计,微板,自动分析仪
检测方法: 吸光度
波长(纳米): 340
信号响应: 减少
线性范围: 每次检测10至150克阿斯巴甜
检测极限: 0.57毫克/升
反应时间(最低): ~5分钟
应用实例: 采购产品软饮料,人造甜味剂,糖果,薄荷,口香糖,饮食产品,果酱,巧克力和其他材料。
方法识别: 新方法

阿斯巴甜试剂盒是测定和分析饮料和食品中阿斯巴甜的一种简便、可靠的方法。

内容说明:如果将所有卷减少一半,每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).

Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
中文名称:天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒
英文名:Aspartame
货号:K-ASPTM
规格:50 assays (manual)
The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials

Principle原理:
(pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH

(dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine

(glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate

(L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+

Kit size:                            50 assays (manual) / 500 (microplate)
/ 500 (auto-analyser)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 5 min
Detection limit:                 0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition:      Novel method

Advantages 优点

Very cost effective

All reagents stable for > 12 months after preparation

Only enzymatic kit available

Measures aspartame and breakdown products (L-aspartate and aspartame acid)

Very specific

Very rapid reaction

Mega-Calc™ software tool is available from our website for hassle-free raw data processing

Standard included

Suitable for manual, microplate and auto-analyser formats