For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit
Colourimetric method for the determination of Yeast and
Mushroom β-Glucan in yeast, mushroom, foodstuffs and
other materials
Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O →
soluble glucan
(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)
(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose
(glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
(amyloglucosidase)
(6) α-Glucan + H2O → D-glucose
Kit size: 100 assays
Method: Spectrophotometric at 510 nm
Total assay time: ~ 100 min
Detection limit: 1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition: Novel method
KITS:
Kits suitable for performing 100 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: (x2) exo-1,3-β-Glucanase (100 U/mL) plus
β-Glucosidase (20 U/mL) ammonium sulphate
suspension, 2.0 mL.
Stable for > 4 years at 4°C.
Bottle 2: Amyloglucosidase (1,630 U/mL) plus invertase
(500 U/mL) solution in 50% (v/v) glycerol, 20 mL.
Stable for ~ 2 years at 4°C or > 4 years below -10°C.
Bottle 3: GOPOD Reagent Buffer.
Buffer (50 mL, pH 7.4).
p-hydroxybenzoic acid and sodium azide (0.09%).
Stable for > 4 years at 4°C.
Bottle 4: GOPOD Reagent Enzymes. Glucose oxidase
plus peroxidase and 4-aminoantipyrine. Freeze-dried
powder. Stable for > 5 years below -10°C.
2
Bottle 5: D-Glucose standard solution (5 mL, 1.00 mg/mL)
in 0.2% (w/v) benzoic acid.
Stable for > 5 years; store sealed at room temperature.
Bottle 6: Control yeast β-glucan preparation (~ 2 g,
β-glucan content stated on the bottle label).
Stable for > 5 years; store sealed at room temperature
试剂盒组成:
Megazyme 提供适合进行 100 次检测的试剂盒。
该试剂盒包含完整的检测方法以及:
瓶 1:(x2)exo-1,3-β-葡聚糖酶(100 U/mL)加上
β-葡萄糖苷酶 (20 U/mL) 硫酸铵
悬浮液,2.0 毫升。
在 4°C 下可稳定 > 4 年。
瓶 2:淀粉葡萄糖苷酶 (1,630 U/mL) 加转化酶
(500 U/mL) 50% (v/v) 甘油溶液,20 mL。
在 4°C 下可稳定约 2 年或在 -10°C 以下可稳定超过 4 年。
瓶 3:GOPOD 试剂缓冲液。
缓冲液(50 毫升,pH 7.4)。
对羟基苯甲酸和叠氮化钠 (0.09%)。
在 4°C 下可稳定 > 4 年。
瓶 4:GOPOD 试剂酶。葡萄糖氧化酶
加上过氧化物酶和4-氨基安替比林。冻干
粉末。 -10°C 以下可稳定保存 > 5 年。
2
第 5 瓶:D-葡萄糖标准溶液(5 mL,1.00 mg/mL)
在 0.2% (w/v) 苯甲酸中。
稳定 > 5 年;室温密封保存。
第 6 瓶:对照酵母 β-葡聚糖制剂(~ 2 g,
瓶标签上注明的β-葡聚糖含量)。
稳定 > 5 年;室温密封保存
Advantages
- Very cost effective
- All reagents stable for > 12 months after preparation
- Only enzymatic kit available
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
Content: |
100 assays per kit |
Shipping Temperature: |
Ambient |
Storage Temperature: |
Short term stability: 2-8oC,
Long term stability: See individual component labels |
Stability: |
> 2 years under recommended storage conditions |
Analyte: |
β-Glucan |
Assay Format: |
Spectrophotometer |
Detection Method: |
Absorbance |
Wavelength (nm): |
510 |
Signal Response: |
Increase |
Linear Range: |
4 to 100 μg of glucose per assay |
Limit of Detection: |
1 g/100 g |
Total Assay Time: |
~ 100 min |
Application examples: |
Yeast preparations, mushroom preparations and other materials. |
Method recognition: |
Novel method |
β-Glucan (Yeast & Mushroom) Assay Kit is suitable for the measurement and analysis of 1,3:1,6-β-glucan and α-glucan in yeast and mushroom preparations.
New, improved procedure.
Several acid and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H2SO4. For most samples, similar β-glucan values were obtained with both the optimized HCl and H2SO4 procedures. However, in the case of certain samples, specifically Ganoderma lucidum and Poria cocus, the H2SO4 procedure resulted in significantly higher values.
McCleary, B.V. & Draga, A. (2016). Measurement of β-Glucan in Mushrooms and Mycelial Products. Journal of AOAC International, Vol. 99, No. 2.
The kit components have not been altered from previous lots.
See our complete list of available polysaccharide assay kits.
Related products
Similar Products
K-EBHLG – Enzymatic Yeast β-Glucan Assay Kit
K-BGLU – β-Glucan Assay Kit (Mixed Linkage)
Related Carbohydrates
P-BGYST – β-Glucan (Yeast; Alkali Soluble)
P-PACHY – Pachyman (1,3-β-D-Glucan)
O-LAM2 – Laminaribiose
O-LAM3 – Laminaritriose
O-LAM4 – Laminaritetraose
O-LAM5 – Laminaripentaose
O-LAM6 – Laminarihexaose
O-LAM7 – Laminariheptaose
O-LAM8 – Laminarioctaose
O-LAM9 – Laminarinonaose
O-GENT – Gentiobiose
Associated Enzymes
E-LAMSE – endo-1,3-β-D-Glucanase (Trichoderma sp.)
E-LAMHV – endo-1,3-β-D-Glucanase (barley)
E-EXBGOS – exo-1,3-β-D-Glucanase + β-Glucosidase
E-EXBGTV – exo-1,3-β-D-Glucanase (Trichoderma virens)
E-BGOSTM – β-Glucosidase (Thermotoga maritima)
E-BGOSAG – β-Glucosidase (Agrobacterium sp.)
E-BGOSPC – β-Glucosidase (Phanerochaete chrysosporium)
E-BGLUC – β-Glucosidase (Aspergillus niger)
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
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