Megazyme K-ASPTM 天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒说明书
天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒
英文名：Aspartame Assay Kit
货号：K-ASPTM
规格：50 assays (manual)
| Content: | 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Aspartame |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 10 to 150 µg of aspartame per assay |
| Limit of Detection: | 0.57 mg/L |
| Reaction Time (min): | ~ 5 min |
| Application examples: | Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic products, jam, chocolate and other materials. |
| Method recognition: | Novel method |
| 内容： | 50项试验(手册)/500项试验(微板)/500项试验(自动分析仪) |
| 船运温度： | 环境 |
| 储存温度： | 短期稳定性：2-8oC， 长期稳定性：见单个组件标签 |
| 稳定性： | >2年在建议的储存条件下 |
| 分析物： | 阿斯巴甜 |
| 化验格式： | 分光光度计，微板，自动分析仪 |
| 检测方法： | 吸光度 |
| 波长(纳米)： | 340 |
| 信号响应： | 减少 |
| 线性范围： | 每次检测10至150克阿斯巴甜 |
| 检测极限： | 0.57毫克/升 |
| 反应时间(最低)： | ~5分钟 |
| 应用实例： | 采购产品软饮料，人造甜味剂，糖果，薄荷，口香糖，饮食产品，果酱，巧克力和其他材料。 |
| 方法识别： | 新方法 |
阿斯巴甜试剂盒是测定和分析饮料和食品中阿斯巴甜的一种简便、可靠的方法。
内容说明：如果将所有卷减少一半，每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
中文名称：天门冬酰苯丙氨酸甲酯/甜味剂/阿斯巴甜[糖精]检测试剂盒
英文名：Aspartame
货号：K-ASPTM
规格：50 assays (manual)
The Aspartame test kit is a simple and reliable method for the specific measurement and analysis of Aspartame in beverages and foodstuffs.
Suitable for manual, auto-analyser and microplate formats.
UV-method for the determination of Aspartame (and breakdown
products) in foodstuffs, beverages and other materials
Principle原理:
(pH 12.5)
(1) Asp-Phe-O-Me → Asp-Phe + MeOH
(dipeptidase M)
(2) Asp-Phe + H2O → L-aspartate + L-phenylalanine
(glutamate-oxaloacetate transaminase)
(3) L-Aspartate + 2-oxoglutarate → L-glutamate + oxaloacetate
(L-malate dehydrogenase)
(4) Oxaloacetate + NADH + H+ → L-malate + NAD+
Kit size: 50 assays (manual) / 500 (microplate)
/ 500 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.57 mg/L
Application examples:
Soft drinks, artificial sweeteners, candies, mints, chewing gum, dietetic
products, jam, chocolate and other materials
Method recognition: Novel method
Advantages 优点
Very cost effective
All reagents stable for > 12 months after preparation
Only enzymatic kit available
Measures aspartame and breakdown products (L-aspartate and aspartame acid)
Very specific
Very rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats
