标签归档：Megazyme酶试剂

Megazyme K-RAFGL 密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒

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Megazyme K-RAFGL 密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

中文名称：密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒
英文名：Raffinose/Sucrose/D-Glucose Assay Kit
货号：K-RAFGL
规格：120 assays of each per kit
品牌： Megazyme

The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Colourimetric method for the determination of Raffinose (also 
stachyose and verbascose), Sucrose and D-Glucose in 
legume seeds, plant materials, foodstuffs and feed

棉子糖/蔗糖/D-葡萄糖检测试剂盒用于测量和分析种子和种子粉中的 D-葡萄糖、蔗糖和棉子糖、水苏糖和毛蕊花糖。 基于 D-葡萄糖对棉子糖、水苏糖和毛蕊花酶水解为 D-葡萄糖、D-果糖和 D-半乳糖的测量。

比色法测定棉子糖（也
水苏糖和毛蕊花）、蔗糖和 D-葡萄糖
豆类种子、植物材料、食品和饲料

Principle:
(α-galactosidase)
(1) Raffinose + stachyose + verbascose + H2O → D-galactose + 
sucrose

(invertase)
(2) Sucrose + H2O → D-glucose + D-fructose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
quinoneimine + 4H2O

Kit size: 120 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Application examples:
Analysis of grain legumes and other materials containing raffinose,
stachyose and verbascose
Method recognition: Used and accepted in food analysis

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme . Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q1。即使对于酸性介质中的样品，也应调整样品的 pH 值吗？

添加样品后检测溶液的 pH 值应与试剂盒随附的检测缓冲液的 pH 值相同。
低样品量（例如 0.1 mL）不太可能影响分析溶液的 pH 值，因此可能不需要调整 pH 值。
超过 0.1 mL 的样品更有可能影响分析溶液的 pH 值，因此在添加到分析之前，应按照数据手册中的说明调整这些样品的 pH 值。

Q2。套件的性能存在问题；结果并不如预期。

如果您怀疑 Megazyme 检测试剂盒未按预期运行，从而无法获得预期结果，请执行以下操作：

确保您已经测试了 Megazyme 测试套件随附的标准样品。
将相关 MegaCalc 电子表格（如果有）中的试剂盒标准品、空白样品和为您的样品获得的结果发送给 Megazyme。相关的 MegaCalc 电子表格可以从 Megazyme 网站上产品出现的地方下载。
说明正在使用的套件批号（可在套件盒的外面找到）。
说明使用了哪种检测格式（如有必要，请参阅试剂盒手册中的相关页面）。
说明对 Megazyme 提供的标准程序的任何修改的确切细节。
说明样品类型并描述样品制备步骤（如果适用）。

Megazyme E-FRMXPD 果聚糖酶混合物

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Megazyme E-FRMXPD 果聚糖酶混合物

Product code: E-FRMXPD
20,000 Units

Content: 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: Supplied as a lyophilised powder
Physical Form: Powder
Stability: Minimum 1 year at < -10oC. Check vial for details.
Enzyme Activity: Fructanase
EC Number: endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
CAZy Family: GH32
CAS Number: 9001-57-4,
9025-67-6,
37288-56-5
Synonyms: endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Source: Aspergillus sp.
Expression: From Aspergillus sp.
Specificity: endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Unit Definition: endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
Temperature Optima: 40oC
pH Optima: 4.5
Method recognition: AOAC Method 997.08, AOAC Method 2000.11, AOAC Method 2016.06, GB Standard 5009.245-2016 and GB Standard 5009.255-2016

Powder (freeze dried)

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Components: exo-inulinase 20,000 U (on kestose, at 40oC), endo-inulinase 1000 U (on fructan at 40oC), α-galactosidase 0.70 U (on p-nitrophenyl α-galactoside at 40oC), β-Glucanase 1.25 U (on β-glucan at 40oC) and pectinase 5.5 U (on pectin at 40oC).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Fructanase and sucrase (E-SUCR) are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.

See more of our other Carbohydrate Active enZYmes products.

Validation of Methods

内容：20,000个单位
运输温度：环境
储存温度：-10oC以下
配方：以冻干粉形式提供
物理形态：粉末
稳定性：在 < -10oC 时至少 1 年。检查小瓶了解详情。
酶活性：果聚糖酶
EC编号：菊粉内切酶：3.2.1.7，
外切菊糖酶：3.2.1.80
CAZy 家族：GH32
CAS 编号：9001-57-4，
9025-67-6,
37288-56-5
同义词：菊粉内切酶：1-β-D-果聚糖果糖水解酶
外切菊糖酶：果聚糖β-果糖苷酶
来源：曲霉属。
表达：来自Aspergillus sp。
特异性：菊粉内切酶：菊粉中 (2,1)-β-D-果糖苷键的内切作用水解。
外切菊糖酶：水解 β-D-呋喃果糖苷中末端的非还原性 β-D-呋喃果糖苷残基。
单位定义：菊粉内切酶：一个菊糖内切酶活性单位定义为在乙酸钠缓冲液中每分钟从菊粉 (20 mg/mL) 中释放 1 μmole β-D-果糖还原糖当量所需的酶量(100 毫米), pH 值 4.5。
外切菊糖酶：一单位外切菊糖酶活性定义为在乙酸钠缓冲液（100 mM）中每分钟从酮糖 (5 mg/mL) 中释放 1 μmole β-D-果糖还原糖当量所需的酶量)，在 40oC 时 pH 值 4.5。
最佳温度：40oC
最佳 pH 值：4.5
方法识别：AOAC Method 997.08、AOAC Method 2000.11、AOAC Method 2016.06、GB Standard 5009.245-2016和GB Standard 5009.255-2016
粉末（冻干）

果聚糖酶、淀粉葡萄糖苷酶 (E-AMGDF) 和异淀粉酶 (E-ISAMY) 用于两种经过验证的食品中聚葡萄糖（一种低摩尔质量膳食纤维）测定方法的酶水解步骤：AOAC 方法 2000.11 和中国 GB 标准 5009.245 -2016 年。

成分：菊糖外切酶 20,000 U（在 40oC 条件下对 kestose），菊粉内切酶 1000 U（在 40oC 条件下对果聚糖），α-半乳糖苷酶 0.70 U（在 40oC 条件下对硝基苯基α-半乳糖苷），β-葡聚糖酶 1.25 U（ β-葡聚糖，40oC）和果胶酶 5.5 U（果胶，40oC）。

注意：本产品已经过纯化，去除了干扰制剂在果聚糖测定（AOAC 方法 997.08）和聚葡萄糖测定（AOAC 方法 2000.11）中使用的 α-半乳糖苷酶、β-葡聚糖酶和果胶酶。

果聚糖酶和蔗糖酶 (E-SUCR) 用于 AOAC 方法 2016.06 和中国 GB 标准 5009.255-2016 测定食品中果聚糖的酶解步骤。

查看更多我们的其他碳水化合物活性酶产品。

Related products  相关产品：
Similar Products
E-FRMXLQ – Fructanase Mixture (liquid)
E-EXOIAN – exo-Inulinase (Aspergillus niger)
E-ENDOIAN – endo-Inulinase (Aspergillus niger)

Related Carbohydrates
P-INUL – Inulin
P-FOS28 – Fructooligosaccharides (FOS)
O-KTR – 1-Kestose
O-KTE – 1,1-Kestotetraose
O-KPE – 1,1,1-Kestopentaose

Colourimetric Substrates
S-AZFR5 – Azo-Fructan
S-AZFRXOI – Azo-Fructan

Associated Enzymes
E-INVPD-2G – Invertase
E-ENLEV – endo-Levanase (Bacteroides thetaiotaomicron)
E-SUCR – Sucrase (from yeast)
E-INVRT – Invertase (fructofuranosidase) (yeast)

Megazyme endo-1,5-α-Arabinanase (Aspergillus niger)

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Megazyme endo-1,5-α-Arabinanase (Aspergillus niger)

上海金畔生物代理Megazyme品牌产品，我们将竭诚为您服务，欢迎访问Megazyme官网或者咨询我们获取更多相关Megazyme品牌产品信息。

内切-1,5- α – 阿拉伯聚糖（黑曲霉）
英文名：endo-1,5-α-Arabinanase (Aspergillus niger)
货号：E-EARAB
规格：400 Units
品牌： Megazyme

Content: 400 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: endo-Arabinanase
EC Number: 3.2.1.99
CAZy Family: GH43
CAS Number: 75432-96-1
Synonyms: arabinan endo-1,5-alpha-L-arabinanase; 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase
Source: Aspergillus niger
Molecular Weight: 39,750
Concentration: Supplied at ~ 200 U/mL
Expression: From Aspergillus niger
Specificity: endo-hydrolysis of (1,5)-α-arabinofuranose linkages in (1,5)-α-arabinans. Acts more slowly on the (1,5)-α-linked arabinan backbone in branched arabinans than on linear arabinans.
Specific Activity: ~ 10 U/mg (40oC, pH 4.0 on CM-linear 1,5-α-L-arabinan)
Unit Definition: One Unit of arabinanase activity is defined as the amount of enzyme required to release one µmole of arabinose reducing-sugar equivalents per minute from CM-linear 1,5-α-L-arabinan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40oC.
Temperature Optima: 40oC
pH Optima: 4
Application examples: Applications established in food and beverage industries, particularly in the reduction of haze in fruit juices and the processing of sugar beet.

 

Megezyme K-PHYTASE 新植酸酶试剂盒

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Megezyme K-PHYTASE 新植酸酶试剂盒

The Phytase Assay Kit (K-PHYTASE) method is a simple, quantitative method which can be used to measure phytase activity.

The Megazyme method has significant advantages over other phytic acid tests that are currently on the market.

Advantages:

The absence of toxic chemicals – A safe and convenient method requiring less reagents preparation and removing the handling of toxic chemicals.
Simpler format – No standard curve required and an enzymatic assay format based on our K-PHOS detection method using a standard UV spectrophotometer.
High sensitivity – Increased sensitivity when compared to routinely used chemical methods.
High repeatability and accuracy – A desalting step can be used to reduce blanks and improve accuracy when measuring low enzyme levels. 

Applications:

Area Application
Animal Feed Manufacturers           To measure phytase levels in animal feed products. Phytases convert the anti-nutrient phytic acid into phosphates which are essential for animal growth. The use of phytases reduces the need for inorganic phosphates addition in feeds which has an impact on the environment.
Enzyme Manufacturers      For the discovery and development of new phytase products and routine phytase analysis.
Research To allow enzyme characterisation and routine analysis in research studies involving the use of phytases.
Commercial Laboratories For the analysis of phytases in animal feed and other matrices. A simpler and safer method that is ideal for high throughput testing.

For more details and to order our new Phytase Assay Kit (K-PHYTASE), click here – Product Code: K-PHYTASE

植酸酶测定试剂盒 (K-PHYTASE) 方法是一种简单的定量方法，可用于测量植酸酶活性。

Megazyme 方法与目前市场上的其他植酸测试相比具有显着优势。

好处：

不含有毒化学物质——一种安全、方便的方法，需要更少的试剂制备和去除有毒化学物质的处理。
更简单的格式——不需要标准曲线，酶分析格式基于我们使用标准紫外分光光度计的 K-PHOS 检测方法。
高灵敏度——与常规使用的化学方法相比，灵敏度更高。
高重复性和准确性 – 在测量低酶水平时，可以使用脱盐步骤来减少空白并提高准确性。

应用：

区域应用
动物饲料制造商 测量动物饲料产品中的植酸酶水平。植酸酶将抗营养植酸转化为对动物生长必不可少的磷酸盐。植酸酶的使用减少了对环境有影响的饲料中添加无机磷酸盐的需要。
酶制造商 用于发现和开发新的植酸酶产品和常规植酸酶分析。
研究 允许在涉及使用植酸酶的研究中进行酶表征和常规分析。
商业实验室 用于分析动物饲料和其他基质中的植酸酶。一种更简单、更安全的方法，是高通量测试的理想选择。

如需更多详细信息并订购我们的新植酸酶检测试剂盒 (K-PHYTASE)，请单击此处 – 产品代码：K-PHYTASE

Megazyme K-MALTA 麦芽淀粉酶检测试剂盒说明书

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Megazyme K-MALTA 麦芽淀粉酶检测试剂盒说明书

麦芽淀粉酶检测试剂盒 K-MALTA Malt Amylase Assay Kit

货号： K-MALTA

品名：麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit

型号：100 assays per kit

内容： 每套试剂盒100份(每套50份)
船运温度： 环境
储存温度： 短期稳定性：2-8oC，
长期稳定性：见单个组件标签
稳定性： >2年在建议的储存条件下
分析物： α-淀粉酶，β-淀粉酶
化验格式： 分光光度计
检测方法： 吸光度
波长(纳米)： 400
信号响应： 增加
检测极限： 0.05U/mL
反应时间(最低)： ~20 min(Ceralpha法)，
~10 min(β-3法)
应用实例： 谷物粉，麦芽，发酵液和其他材料。
方法识别： “Ceralpha”方法：AACC方法22-02.01，AOAC方法2002.01，ICC标准编号303号，RACI标准方法和CCFRA(面粉测试工作组方法0018)。“β-3”法：RACI标准法

α-淀粉酶，β-淀粉酶在淀粉降解代谢过程中或发酵糖发芽或谷物麦芽中发挥重要作用。

上海金畔生物提供麦芽淀粉酶检测试剂盒，比色法（400nm）测定谷物、麦芽、食品、饮料和发酵产品中的α-淀粉酶和Beta-淀粉酶。

scheme-K-MALTA MALTA MEgazyme

(1) α-淀粉酶检测利用“Ceralpha” 方法

(2) Beta-淀粉酶检测利用 “Betamyl-3” 方法

反应时间：

麦芽淀粉酶检测试剂盒 K-MALTA 
~ 20 min (Ceralpha 方法)

~ 10 min (Betamyl-3 方法)

检测限：0.05 U/mL

适用样品：面粉、麦芽、发酵液和其他原料

Advantages  优点：
  • Very cost effective
  • All reagents stable for > 2 years as supplied
  • Only enzymatic kit available (Beta-Amylase)
  • Very specific
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

价格低廉（每次检测成本）

所有试剂配制后的稳定性>2年

只适用于酶法试剂盒

特异性高

方法简单

包含标准品

Related products
Similar Products
K-CERA – α-Amylase Assay Kit (Ceralpha Method)
K-AMYLSD – α-Amylase SD Assay Kit
K-MALTA – Malt Amylase Assay Kit
K-BETA3 – β-Amylase Assay Kit (Betamyl-3)
R-BAMR3 – β-Amylase Assay Reagent (Betamyl-3)
R-CAAR4 – α-Amylase Reagent (Ceralpha)
Related Carbohydrates
P-AMYL – Amylose (potato)
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
Colourimetric Substrates
T-AMZ-200T – Amylazyme Tablets
S-RSTAR – Red Starch
I-AZAMY – AZCL-Amylose
O-BPNPC7 – Blocked 4-nitrophenyl-α-maltoheptaoside
Associated Enzymes
E-MAST – Malt Amylase Standard
E-MASTP – Malt Amylase Standard (powder)
E-BARBL-50KU – β-Amylase (Barley)
E-BARBP-2G – β-Amylase (Barley) Powder
E-BAMBC – β-Amylase (Bacillus cereus)

Megazyme E-EGALN 1,4-β-D-半乳糖内切酶[黑曲霉]

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Megazyme E-EGALN  1,4-β-D-半乳糖内切酶[黑曲霉]
Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

中文名称：1，4-b-D-半乳糖内切酶[黑曲霉]

英文名：endo-1,4-β-Galactanase (Aspergillus niger)

货号：E-EGALN

规格：1000 Units

endo-1,4-β-Galactanase (Aspergillus niger)

High purity endo-1,4-β-Galactanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.89
CAZy Family: GH53
CAS: 58182-40-4

arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase

From Aspergillus niger.
In 3.2 M ammonium sulphate.
Supplied at ~ 1,300 U/mL.

Specific activity:
> 150 U/mg (40oC, pH 4.0 on potato galactan).

Stability: > 4 years at 4oC.

Content: 1,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: endo-1,4-β-Galactanase
EC Number: 3.2.1.89
CAZy Family: GH53
CAS Number: 58182-40-4
Synonyms: arabinogalactan endo-beta-1,4-galactanase; arabinogalactan 4-beta-D-galactanohydrolase
Source: Aspergillus niger
Molecular Weight: 48,000
Expression: From Aspergillus niger
Specificity: endo-hydrolysis of (1,4)-β-D-galactose linkages in (1,4)-β-galactans and type I arabinogalactans.
Specific Activity: > 150 U/mg (40oC, pH 4.0 on potato galactan)
Unit Definition: One Unit of galactanase activity is defined as the amount of enzyme required to release one µmole of galactose reducing-sugar equivalents per minute from potato galactan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40oC.

Megazyme K-YBGL Beta 葡聚糖[酵母和蘑菇]检测试剂盒说明书

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Megazyme K-YBGL Beta 葡聚糖[酵母和蘑菇]检测试剂盒说明书

Beta葡聚糖[酵母和蘑菇]检测试剂盒 K-YBGL

β-Glucan Assay Kit (Yeast & Mushroom)

产品名称：Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名称：Mushroom and yeast beta-glucan assay Kit

货号： K-YBGL

型号规格：100次

优点：成本低；所有试剂配制后的稳定性〉2年；只有酶试剂盒可用；；方法简单；包含标准品

检测原理：

检测方法：比色法

检测波长：510nm；

总检测时间：100min；

检测限：样品重量的1-100%；

适用样品：酵母制备、蘑菇制备及其他原料；

方法认证：新方法

商品说明：

For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit

Colourimetric method for the determination of Yeast and 
Mushroom β-Glucan in yeast, mushroom, foodstuffs and 
other materials

Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O → 
soluble glucan

(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)

(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose

(glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
 quinoneimine + 4H2O

(amyloglucosidase)
(6) α-Glucan + H2O    →    D-glucose

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition:        Novel method

KITS:
Kits suitable for performing 100 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: (x2) exo-1,3-β-Glucanase (100 U/mL) plus
β-Glucosidase (20 U/mL) ammonium sulphate
suspension, 2.0 mL.
Stable for > 4 years at 4°C.
Bottle 2: Amyloglucosidase (1,630 U/mL) plus invertase
(500 U/mL) solution in 50% (v/v) glycerol, 20 mL.
Stable for ~ 2 years at 4°C or > 4 years below -10°C.
Bottle 3: GOPOD Reagent Buffer.
Buffer (50 mL, pH 7.4).
p-hydroxybenzoic acid and sodium azide (0.09%).
Stable for > 4 years at 4°C.
Bottle 4: GOPOD Reagent Enzymes. Glucose oxidase
plus peroxidase and 4-aminoantipyrine. Freeze-dried
powder. Stable for > 5 years below -10°C.
2
Bottle 5: D-Glucose standard solution (5 mL, 1.00 mg/mL)
in 0.2% (w/v) benzoic acid.
Stable for > 5 years; store sealed at room temperature.
Bottle 6: Control yeast β-glucan preparation (~ 2 g,
β-glucan content stated on the bottle label).
Stable for > 5 years; store sealed at room temperature

试剂盒组成：
Megazyme 提供适合进行 100 次检测的试剂盒。
该试剂盒包含完整的检测方法以及：
瓶 1：（x2）exo-1,3-β-葡聚糖酶（100 U/mL）加上
β-葡萄糖苷酶 (20 U/mL) 硫酸铵
悬浮液，2.0 毫升。
在 4°C 下可稳定 > 4 年。
瓶 2：淀粉葡萄糖苷酶 (1,630 U/mL) 加转化酶
(500 U/mL) 50% (v/v) 甘油溶液，20 mL。
在 4°C 下可稳定约 2 年或在 -10°C 以下可稳定超过 4 年。
瓶 3：GOPOD 试剂缓冲液。
缓冲液（50 毫升，pH 7.4）。
对羟基苯甲酸和叠氮化钠 (0.09%)。
在 4°C 下可稳定 > 4 年。
瓶 4：GOPOD 试剂酶。葡萄糖氧化酶
加上过氧化物酶和4-氨基安替比林。冻干
粉末。 -10°C 以下可稳定保存 > 5 年。
2
第 5 瓶：D-葡萄糖标准溶液（5 mL，1.00 mg/mL）
在 0.2% (w/v) 苯甲酸中。
稳定 > 5 年；室温密封保存。
第 6 瓶：对照酵母 β-葡聚糖制剂（~ 2 g，
瓶标签上注明的β-葡聚糖含量）。
稳定 > 5 年；室温密封保存

Advantages

  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Only enzymatic kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
Content: 100 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: β-Glucan
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 510
Signal Response: Increase
Linear Range: 4 to 100 μg of glucose per assay
Limit of Detection: 1 g/100 g
Total Assay Time: ~ 100 min
Application examples: Yeast preparations, mushroom preparations and other materials.
Method recognition: Novel method

β-Glucan (Yeast & Mushroom) Assay Kit is suitable for the measurement and analysis of 1,3:1,6-β-glucan and α-glucan in yeast and mushroom  preparations.

New, improved procedure.

Several acid and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H2SO4. For most samples, similar β-glucan values were obtained with both the optimized HCl and H2SO4 procedures. However, in the case of certain samples, specifically Ganoderma lucidum and Poria cocus, the H2SO4 procedure resulted in significantly higher values.

McCleary, B.V. & Draga, A. (2016). Measurement of β-Glucan in Mushrooms and Mycelial Products. Journal of AOAC International, Vol. 99, No. 2.

The kit components have not been altered from previous lots.

See our complete list of available polysaccharide assay kits.

scheme-K-YBGL YBGL Megazyme

Related products
Similar Products
K-EBHLG – Enzymatic Yeast β-Glucan Assay Kit
K-BGLU – β-Glucan Assay Kit (Mixed Linkage)
Related Carbohydrates
P-BGYST – β-Glucan (Yeast; Alkali Soluble)
P-PACHY – Pachyman (1,3-β-D-Glucan)
O-LAM2 – Laminaribiose
O-LAM3 – Laminaritriose
O-LAM4 – Laminaritetraose
O-LAM5 – Laminaripentaose
O-LAM6 – Laminarihexaose
O-LAM7 – Laminariheptaose
O-LAM8 – Laminarioctaose
O-LAM9 – Laminarinonaose
O-GENT – Gentiobiose
Associated Enzymes
E-LAMSE – endo-1,3-β-D-Glucanase (Trichoderma sp.)
E-LAMHV – endo-1,3-β-D-Glucanase (barley)
E-EXBGOS – exo-1,3-β-D-Glucanase + β-Glucosidase
E-EXBGTV – exo-1,3-β-D-Glucanase (Trichoderma virens)
E-BGOSTM – β-Glucosidase (Thermotoga maritima)
E-BGOSAG – β-Glucosidase (Agrobacterium sp.)
E-BGOSPC – β-Glucosidase (Phanerochaete chrysosporium)
E-BGLUC – β-Glucosidase (Aspergillus niger)
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
Related Products
D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer)

Megazyme K-GCROL 甘油检测试剂盒中文说明书

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Megazyme K-GCROL 甘油检测试剂盒 Glycerol GK Assay Kit

甘油检测试剂盒 Glycerol GK Assay Kit

产品货号：K-GCROL

产品品名： 甘油检测试剂盒

英文品名 ：Glycerol Assay Kit

规格型号： 70 assays per kit

甘油是食品加工业中通常使用的甜味剂和保湿剂，大多出现在运动食品和代乳品中。

上海金畔生物提供 甘油检测试剂盒 ，紫外吸光度法（ 340nm ）测定食品、饮料及其他原料中的甘油。

原理：

反应时间： 5min

检测限： 0.37mg/L

适用样品： 葡萄酒（葡萄汁）、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸（硬纸板）、化妆品

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+

Kit size: 70 assays (manual) / 700 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK

Content: 70 assays (manual) / 700 assays (microplate)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Glycerol
Assay Format: Spectrophotometer, Microplate
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Decrease
Linear Range: 0.8 to 35 µg of glycerol per assay
Limit of Detection: 0.34 mg/L
Reaction Time (min): ~ 5 min
Application examples: Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices, soft drinks, toothpaste, honey, tobacco, paper (and cardboard), cosmetics, pharmaceuticals, soap and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Methods based on this principle have been accepted by OIV and MEBAK

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

Browse our wide range of alcohol assay kit products.

Scheme-K-GCROL GCROL Megazyme

Advantages
  • Novel format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual and microplate formats
  • Extended cofactors stability
内容： 70次化验(手册)/700次试验(微版)
船运温度： 环境
储存温度： 短期稳定性：2-8oC，
长期稳定性：见单个组件标签
稳定性： >2年在建议的储存条件下
分析物： 甘油
化验格式： 分光光度计，微版
检测方法： 吸光度
波长(纳米)： 340
信号响应： 减少
线性范围： 每次测定甘油0.8至35微克
检测极限： 0.34毫克/升
反应时间(最低)： ~5分钟
应用实例： 葡萄酒(和葡萄汁)、啤酒、烈酒、醋、杏仁锅、果汁、软饮料、牙膏、蜂蜜、烟草、纸张(和纸板)、化妆品、药品、肥皂和其他材料(例如生物培养物、样品等)。
方法识别： 基于这一原则的方法已被OIV和MEBAK所接受。

甘油检测试剂盒是一种简单、可靠、快速、准确的测定和分析饮料、食品和其他材料中甘油的方法。

内容说明：如果将所有卷减少一半，每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).

浏览我们范围广泛的酒精分析试剂盒产品。

Scheme-K-GCROL GCROL Megazyme

优势
  • 增加稳定性的新格式
  • 极具竞争力的价格(每次测试成本)
  • 所有试剂按供应情况稳定超过2年
  • 极快速反应
  • 特尔加-卡尔克™软件工具可从我们的网站获得无麻烦的原始数据处理。
  • 标准包括
  • 适用于手动和微型制版

Megazyme E-ANAAM α-淀粉酶（米曲霉）

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Megazyme E-ANAAM α-淀粉酶（米曲霉）
Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

英文名：α-Amylase (Aspergillus oryzae)
货号：E-ANAAM
规格：20,000 Units
品牌： Megazyme

alpha-Amylase Aspergillus oryzae E-ANAAM
alpha-Amylase Aspergillus oryzae E-ANAAM Scheme
Content: 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: α-Amylase
EC Number: 3.2.1.1
CAZy Family: GH13
CAS Number: 9000-90-2
Synonyms: alpha-amylase; 4-alpha-D-glucan glucanohydrolase
Source: Aspergillus oryzae
Molecular Weight: 54,000
Concentration: Supplied at ~ 1,000 CU/mL
Expression: From Aspergillus oryzae
Specificity: endo-hydrolysis of α-1,4-D-glucosidic linkages in starch.
Specific Activity: ~ 120 U/mg (40oC, pH 5.4 on Ceralpha Reagent)
Unit Definition: One Unit of α-amylase is the amount of enzyme required to release one µmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase), pH 5.4 at 40oC, and is termed a Ceralpha Unit.
Temperature Optima: 50oC
pH Optima: 5
Application examples: For use in Megazyme Starch Damage method.

Related products   其他相关产品：
Similar Products
E-BAASS – α-Amylase (Bacillus amyloliquefaciens)
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis)
E-MAST – Malt Amylase Standard
E-MASTP – Malt Amylase Standard (powder)
Related Carbohydrates
P-AMYL – Amylose (potato)
P-BLDX-10G – β-Limit Dextrin
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
Colourimetric Substrates
K-AMYLSD – α-Amylase SD Assay Kit
K-CERA – α-Amylase Assay Kit (Ceralpha Method)
K-MALTA – Malt Amylase Assay Kit
T-AMZ-200T – Amylazyme Tablets
S-RSTAR – Red Starch
I-AZAMY – AZCL-Amylose
O-BPNPC7 – Blocked 4-nitrophenyl-α-maltoheptaoside
Associated Enzymes
E-BARBL-50KU – β-Amylase (Barley)
E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)
E-PULKP – Pullulanase M1 (Klebsiella planticola)
E-ISAMYHP – Isoamylase HP (Glycogen 6-glucanohydrolase)
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)

Megazyme R-AMGR3 淀粉葡萄糖苷酶检测试剂中文说明书

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Megazyme R-AMGR3 淀粉葡萄糖苷酶检测试剂中文说明书

淀粉葡萄糖苷酶测定试剂
英文名：Amyloglucosidase Assay Reagent
货号：R-AMGR3
规格：200次检测Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

Content: 200 assays per kit (4 vials)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Amyloglucosidase
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 400
Limit of Detection: ~ 2.5 U/mL
Reproducibility (%): ~ 3%
Reaction Time (min): ~ 15 min
内容： 每盒200次化验(4小瓶)
船运温度： 环境
储存温度： 短期稳定性：2-8oC，
长期稳定性：见单个组件标签
稳定性： >2年在建议的储存条件下
分析物： 氨基葡萄糖苷酶
化验格式： 分光光度计
检测方法： 吸光度
波长(纳米)： 400
检测极限： ~2.5U/mL
可重复性(%)： ~ 3%
反应时间(最低)： ~15分钟

高纯度淀粉葡萄糖苷酶分析试剂-4瓶，用于淀粉糖苷酶的测定，用于研究，生化酶分析和离体诊断分析

p-硝基苯β-D-麦芽苷加过量的β-葡萄糖苷酶.

Related Carbohydrates
P-AMYL – Amylose (potato)
O-PAN-1G – Panose
O-GMT – 63-α-D-Glucosyl-maltotriose
O-GMH – 63-α-D-Glucosyl-maltotriosyl-maltotriose
O-MTMT – 63-α-D-Maltotriosyl-maltotriose
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
O-IPAN – Isopanose
Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
E-AMGDFNG-20ML – Amyloglucosidase (Aspergillus niger)
Glycerol Free
E-AMGDFPD – Amyloglucosidase (Aspergillus niger) Powder
E-AMGFR-100MG – Amyloglucosidase (Aspergillus niger)
E-AMGPU – Amyloglucosidase (Rhizopus sp.)

上海金畔生物代理Megazyme品牌试剂产品，欢迎新老客户访问Megazyme官网或者咨询我们获取更多相关产品信息。
图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品（包括但不限于抗生素类、蛋白质类、试剂盒类产品等）仅限用于科学研究用途，不得作用于人体。