600 Units
Prices exclude VAT
Available for shipping
North American customers click here
Content: | 600 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 4 years at 4oC |
Enzyme Activity: | Isoamylase |
EC Number: | 3.2.1.68 |
CAZy Family: | GH13 |
CAS Number: | 9067-73-6 |
Synonyms: | isoamylase; glycogen 6-alpha-D-glucanohydrolase |
Source: | Pseudomonas sp. |
Molecular Weight: | 71,500 |
Concentration: | Supplied at ~ 200 U/mL |
Expression: | Purified from Pseudomonas sp. |
Specificity: | Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. |
Specific Activity: | ~ 180 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg) |
Unit Definition: | One unit of isoamylase activity is the amount of enzyme required to release one µmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC. |
Temperature Optima: | 50oC |
pH Optima: | 4 |
Application examples: | Applications in carbohydrate research and in the food and feeds, and cereals industry. |
Method recognition: | AOAC Method 2000.11 and GB Standard 5009.245-2016 |
Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Please refer to E-ISAMYHP for high purity enzyme suitable for use in starch structural research.
We have a wide range of other CAZyme products available.
Validation of Methods
FAQs Data Sheet
Symbol : | Not Applicable |
Signal Word : | Not Applicable |
Hazard Statements : | Not Applicable |
Precautionary Statements : | Not Applicable |
Megazyme产品应用分类:
食品检测 饲料检测 发酵业检测 红酒业检测 酿造业检测 奶制品检测 生物燃料 食物育种 实验室分析 研究
如果需要咨询Megazyme试剂盒说明书等其他技术问题可以访问Megazyme官网或者联系我们。