1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
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| Content: | 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Glucose |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance, Fluorescence |
| Wavelength (nm): | (Fluorescence) 530-560 (ex), ~ 590 (em) (Absorbance) 570 |
| Signal Response: | Increase |
| Linear Range: | (Fluorescence) 0.009-0.18 μg of D-glucose per assay (0.5-10 μM in-assay) (Absorbance) 0.9-9.0 μg of D-glucose per assay (5-50 μM in-assay) |
| Limit of Detection: | (Fluorescence) 0.09 ug/mL (Absorbance) 0.25 ug/mL |
| Reproducibility (%): | (Fluorescence) ~ 6% (Absorbance) ~ 7% |
| Reaction Time (min): | (Fluorescence) 30 min (Absorbance) 30 min |
| Application examples: | Food and beverage samples such as wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables. Other samples such as tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.). |
内容：1000 次检测（微孔板）/100 次检测（手动）/400 次检测（自动分析仪）
运输温度：环境
储存温度：短期稳定性：2-8oC，
长期稳定性：见单个组件标签
稳定性：在推荐的储存条件下 > 2 年
分析物：D-葡萄糖
检测形式：分光光度计、微孔板、自动分析仪
检测方法：吸光度、荧光
波长 (nm)：（荧光）530-560 (ex), ~ 590 (em)
（吸光度）570
信号响应：增加
线性范围：（荧光）每次测定 0.009-0.18 μg D-葡萄糖（测定中 0.5-10 μM）
（吸光度）每次测定 0.9-9.0 μg D-葡萄糖（测定中 5-50 μM）
检测限：（荧光）0.09 ug/mL
（吸光度）0.25 ug/mL
重现性 (%): (荧光) ~ 6%
（吸光度）~ 7%
反应时间（分钟）：（荧光）30 分钟
（吸光度）30 分钟
应用示例：食品和饮料样品，如葡萄酒、啤酒、果汁、软饮料、牛奶、果酱、营养食品、烘焙产品、糖果、水果和蔬菜。其他样品，例如烟草、化妆品、药品（例如输液）、饲料、纸张（和纸板）和其他材料（例如生物培养物、样品等）。
The D-Glucose Assay Kit (Megaplex Red) provides a simple robust method for the measurement of D-glucose. This kit utilises a highly sensitive Megaplex Red probe which when coupled to horseradish peroxidase (HRP) and glucose oxidase (GOX) enzymatic reactions, allows for the measurement of D-glucose in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation between 530-560 nm and emission at ~ 590 nm* or colourimetrically at 570 nm.
*The excitation and emission maxima of Megaplex Red are 570 nm and 585 nm respectively.
D-葡萄糖检测试剂盒（Megaplex Red）为测量 D-葡萄糖提供了一种简单可靠的方法。 该试剂盒使用高度灵敏的 Megaplex Red 探针，当与辣根过氧化物酶 (HRP) 和葡萄糖氧化酶 (GOX) 酶促反应偶联时，可以测量样品中的 D-葡萄糖。 通过 HRP 氧化探针后，形成有色产物（试卤灵），可以使用 530-560 nm 之间的激发和 ~ 590 nm* 的发射或在 570 nm 的比色法在荧光模式下测量。
* Megaplex Red 的激发和发射最大值分别为 570 nm 和 585 nm。
See more related mono/disaccharide assay kit products.
Fluorometric/UV method for the determination of D-glucose content in a variety of samples.
- Simple format
- Very competitive price (cost per test)
- Ability to run in fluorescence or absorbance mode
- User friendly – Detailed protocol provided for the creation of calibration curve and the calculation of concentration in fluorescence mode. Single point standard for quicker and simpler analysis in absorbance mode.
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Suitable for manual, microplate and auto-analyser formats
- All reagents stable for > 2 years
Certificate of Analysis
Assay Protocol Data Calculator
类似产品
K-GLUHK-220A – D-Glucose HK Assay Kit K-GLUC – D-Glucose Assay Kit（GOPOD 格式） K-FRUGL – D-Fructose/D-Glucose Assay Kit K-FRGLQR – D-Fructose/D-Glucose Assay 试剂盒（液体就绪）K-LACSU – 乳糖/蔗糖/D-葡萄糖检测试剂盒 K-MASUG – 麦芽糖/蔗糖/D-葡萄糖检测试剂盒 K-MANGL – D-甘露糖/D-果糖/D-葡萄糖检测试剂盒 K- RAFGL – 棉子糖/蔗糖/D-葡萄糖检测试剂盒 K-SUFRG – 蔗糖/D-果糖/D-葡萄糖检测试剂盒 K-SUCGL – 蔗糖/D-葡萄糖检测试剂盒
相关产品
D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack（化学分析仪） K-MRH2O2 – 过氧化氢检测试剂盒（Megaplex Red） E-GOXCA – 葡萄糖氧化酶 – 过氧化氢酶混合物（真核生物）
| Symbol : | GHS06 |
| Signal Word : | Danger |
| Hazard Statements : | H301 |
| Precautionary Statements : | P264, P270, P301+P310, P321, P330 |
Megazyme产品应用分类：
食品检测 饲料检测 发酵业检测 红酒业检测 酿造业检测 奶制品检测 生物燃料 食物育种 实验室分析 研究
如果需要咨询Megazyme试剂盒说明书等其他技术问题可以访问Megazyme官网或者联系我们。