The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.
Colourimetric method for the determination of Raffinose (also
stachyose and verbascose), Sucrose and D-Glucose in
legume seeds, plant materials, foodstuffs and feed
棉子糖/蔗糖/D-葡萄糖检测试剂盒用于测量和分析种子和种子粉中的 D-葡萄糖、蔗糖和棉子糖、水苏糖和毛蕊花糖。 基于 D-葡萄糖对棉子糖、水苏糖和毛蕊花酶水解为 D-葡萄糖、D-果糖和 D-半乳糖的测量。
比色法测定棉子糖（也
水苏糖和毛蕊花）、蔗糖和 D-葡萄糖
豆类种子、植物材料、食品和饲料
Principle:
(α-galactosidase)
(1) Raffinose + stachyose + verbascose + H2O → D-galactose +
sucrose
(invertase)
(2) Sucrose + H2O → D-glucose + D-fructose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Kit size: 120 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Application examples:
Analysis of grain legumes and other materials containing raffinose,
stachyose and verbascose
Method recognition: Used and accepted in food analysis
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Simple format
- Rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
Q1. Should the pH of the sample be adjusted even for samples in acidic media?
The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.
Q2. There is an issue with the performance of the kit; the results are not as expected.
If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:
- Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
- Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme . Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
- State the kit lot number being used (this is found on the outside of the kit box).
- State which assay format was used (refer to the relevant page in the kit booklet if necessary).
- State exact details of any modifications to the standard procedure that is provided by Megazyme.
- State the sample type and describe the sample preparation steps if applicable.
Q1。即使对于酸性介质中的样品，也应调整样品的 pH 值吗？
添加样品后检测溶液的 pH 值应与试剂盒随附的检测缓冲液的 pH 值相同。
低样品量（例如 0.1 mL）不太可能影响分析溶液的 pH 值，因此可能不需要调整 pH 值。
超过 0.1 mL 的样品更有可能影响分析溶液的 pH 值，因此在添加到分析之前，应按照数据手册中的说明调整这些样品的 pH 值。
Q2。套件的性能存在问题；结果并不如预期。
如果您怀疑 Megazyme 检测试剂盒未按预期运行，从而无法获得预期结果，请执行以下操作：
确保您已经测试了 Megazyme 测试套件随附的标准样品。
将相关 MegaCalc 电子表格（如果有）中的试剂盒标准品、空白样品和为您的样品获得的结果发送给 Megazyme。相关的 MegaCalc 电子表格可以从 Megazyme 网站上产品出现的地方下载。
说明正在使用的套件批号（可在套件盒的外面找到）。
说明使用了哪种检测格式（如有必要，请参阅试剂盒手册中的相关页面）。
说明对 Megazyme 提供的标准程序的任何修改的确切细节。
说明样品类型并描述样品制备步骤（如果适用）。
