Total Dietary Fiber Assay Kit,根据AOAC和AACC批准的方法测量和分析总,可溶性和不溶性膳食纤维。参见总裁判报告:AOAC INTERNATIONAL杂志,Vol。81,No.1,1998。
纤维是复杂有机物质的混合物,包括亲水性化合物,例如可溶性和不可溶性多糖和不可消化的低聚糖,以及一系列不可溶胀的或多或少疏水性的化合物,如角质素,软木脂和木质素。我们的小册子中概述的总膳食纤维测定和分析程序是基于Lee 等人的方法。1 和普罗斯盖等。2,3 (AOAC 991.43,AOAC 985.29,AACC 32-07.01和AACC 32-05.01)。然而,Megazyme总膳食纤维套件中的酶也可用于其他膳食纤维分析方法,如AACC方法32-21.01和AACC方法32-06.01。
1.分析化学家协会。(1985)。Official Methods of Analysis,14th ed。,1st suppl。秒。43,A14-43,A20,p.399。
2.分析化学家协会。(1986年)。方法的变化。J. Assoc。关闭。肛门。化学。,69,370。
基于AOAC方法991.43“食品中总量,可溶性和不溶性膳食纤维”(First Action 1991)和AACC Method 32-07.01“食品和食品中可溶性,不溶性和总膳食纤维的测定”(zui终批准10- 16-91)。
The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods.
Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.
Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.
1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.
See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
Two separate methods are described in the associated assay protocol:
METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).
METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER
Based on AACC method 32-05.01 and AOAC Method 985.29.
Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.
内容: |
100次/200次 |
船运温度: |
环境 |
储存温度: |
短期稳定性:2-8oC,
长期稳定性:见单个组件标签 |
稳定性: |
>2年在建议的储存条件下 |
分析物: |
膳食纤维 |
化验格式: |
酶学 |
检测方法: |
重量法 |
信号响应: |
增加 |
检测极限: |
0.5克/100克 |
试验总时间: |
~100分钟 |
应用实例: |
食品配料、食品及其他材料。 |
方法识别: |
AACC方法32-05.01,AACC方法32-06.01,AACC方法32-07.01,AACC方法32-21.01,aac方法985.29,aac方法991.42,aac方法991.43,aac方法993.19,Codex方法I和GB标准5009.88-2014。 |
总膳食纤维分析试剂盒对总膳食纤维、可溶性和不溶性膳食纤维的分析 根据AOAC和AACC批准的方法。
膳食纤维一般可以被描述为食物中碳水化合物的含量,而这些碳水化合物在人类小肠中是不被消化的。它通过进入大肠,在那里它是部分或完全发酵。膳食纤维的这些特性与其大量的健康益处有着密切的关系。
膳食纤维是一种复杂的有机物质的混合物,包括水溶性和不溶性多糖和不可消化的低聚糖等亲水化合物,以及一系列不可膨胀、或多或少疏水的化合物,如木质素、木质素和木质素。测定和分析总膳食纤维的方法是基于Lee的方法。等人.1普洛斯基等人.2,3(AOAC 991.43,AOAC 985.29,AACC 32-07.01和AACC 32-05.01)。然而,大麦草总膳食纤维试剂盒中的酶也可用于其他膳食纤维分析方法,如AACC法32-21.01和AACC法32-06.01。
1.官方分析化验师协会。(1985年)。官方分析方法,第14版,第一补编。赛斯。43,A14-43,A20,第399页。
2.官方分析化验师协会。(1986年)。方法的改变。J.Assoc.关掉。肛门。化学。, 69, 370.
3.官方分析化验师协会。(1987年)。方法的改变。J.Assoc.关掉。肛门。化学., 70, 393.
见总裁判报告:AOAC国际杂志,第81卷,第1号,1998年。
在相关的检测协议中描述了两种不同的方法:
方法1:总膳食纤维、可溶性膳食纤维和不溶性膳食纤维的测定
根据美国aac方法991.43“食品中的总、可溶性和不溶性膳食纤维”(1991年第一行动)和AACC方法32-07.01“食品和食品中可溶性、不溶性和总膳食纤维的测定”(最终批准10-16-91)。
方法2:膳食纤维总量的测定
基于AACC方法32-05.01和AOAC方法985.29.
请注意,原始方法开发人员LeonProsky博士的背书包含在DocumentsTab中。
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