Megazyme E-FRMXPD 果聚糖酶混合物

Megazyme E-FRMXPD 果聚糖酶混合物

Product code: E-FRMXPD
20,000 Units

Content: 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: Supplied as a lyophilised powder
Physical Form: Powder
Stability: Minimum 1 year at < -10oC. Check vial for details.
Enzyme Activity: Fructanase
EC Number: endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
CAZy Family: GH32
CAS Number: 9001-57-4,
9025-67-6,
37288-56-5
Synonyms: endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Source: Aspergillus sp.
Expression: From Aspergillus sp.
Specificity: endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Unit Definition: endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
Temperature Optima: 40oC
pH Optima: 4.5
Method recognition: AOAC Method 997.08, AOAC Method 2000.11, AOAC Method 2016.06, GB Standard 5009.245-2016 and GB Standard 5009.255-2016

Powder (freeze dried)

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Componentsexo-inulinase 20,000 U (on kestose, at 40oC), endo-inulinase 1000 U (on fructan at 40oC), α-galactosidase 0.70 U (on p-nitrophenyl α-galactoside at 40oC), β-Glucanase 1.25 U (on β-glucan at 40oC) and pectinase 5.5 U (on pectin at 40oC).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Fructanase and sucrase (E-SUCR) are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.

See more of our other Carbohydrate Active enZYmes products.

Validation of Methods

内容:20,000个单位
运输温度:环境
储存温度:-10oC以下
配方:以冻干粉形式提供
物理形态:粉末
稳定性:在 < -10oC 时至少 1 年。检查小瓶了解详情。
酶活性:果聚糖酶
EC编号:菊粉内切酶:3.2.1.7,
外切菊糖酶:3.2.1.80
CAZy 家族:GH32
CAS 编号:9001-57-4,
9025-67-6,
37288-56-5
同义词:菊粉内切酶:1-β-D-果聚糖果糖水解酶
外切菊糖酶:果聚糖β-果糖苷酶
来源:曲霉属。
表达:来自Aspergillus sp。
特异性:菊粉内切酶:菊粉中 (2,1)-β-D-果糖苷键的内切作用水解。
外切菊糖酶:水解 β-D-呋喃果糖苷中末端的非还原性 β-D-呋喃果糖苷残基。
单位定义:菊粉内切酶:一个菊糖内切酶活性单位定义为在乙酸钠缓冲液中每分钟从菊粉 (20 mg/mL) 中释放 1 μmole β-D-果糖还原糖当量所需的酶量(100 毫米), pH 值 4.5。
外切菊糖酶:一单位外切菊糖酶活性定义为在乙酸钠缓冲液(100 mM)中每分钟从酮糖 (5 mg/mL) 中释放 1 μmole β-D-果糖还原糖当量所需的酶量),在 40oC 时 pH 值 4.5。
最佳温度:40oC
最佳 pH 值:4.5
方法识别:AOAC Method 997.08、AOAC Method 2000.11、AOAC Method 2016.06、GB Standard 5009.245-2016和GB Standard 5009.255-2016
粉末(冻干)

果聚糖酶、淀粉葡萄糖苷酶 (E-AMGDF) 和异淀粉酶 (E-ISAMY) 用于两种经过验证的食品中聚葡萄糖(一种低摩尔质量膳食纤维)测定方法的酶水解步骤:AOAC 方法 2000.11 和中国 GB 标准 5009.245 -2016 年。

成分:菊糖外切酶 20,000 U(在 40oC 条件下对 kestose),菊粉内切酶 1000 U(在 40oC 条件下对果聚糖),α-半乳糖苷酶 0.70 U(在 40oC 条件下对硝基苯基α-半乳糖苷),β-葡聚糖酶 1.25 U( β-葡聚糖,40oC)和果胶酶 5.5 U(果胶,40oC)。

注意:本产品已经过纯化,去除了干扰制剂在果聚糖测定(AOAC 方法 997.08)和聚葡萄糖测定(AOAC 方法 2000.11)中使用的 α-半乳糖苷酶、β-葡聚糖酶和果胶酶。

果聚糖酶和蔗糖酶 (E-SUCR) 用于 AOAC 方法 2016.06 和中国 GB 标准 5009.255-2016 测定食品中果聚糖的酶解步骤。

查看更多我们的其他碳水化合物活性酶产品。

Related products  相关产品:
Similar Products
E-FRMXLQ – Fructanase Mixture (liquid)
E-EXOIAN – exo-Inulinase (Aspergillus niger)
E-ENDOIAN – endo-Inulinase (Aspergillus niger)

Related Carbohydrates
P-INUL – Inulin
P-FOS28 – Fructooligosaccharides (FOS)
O-KTR – 1-Kestose
O-KTE – 1,1-Kestotetraose
O-KPE – 1,1,1-Kestopentaose

Colourimetric Substrates
S-AZFR5 – Azo-Fructan
S-AZFRXOI – Azo-Fructan

Associated Enzymes
E-INVPD-2G – Invertase
E-ENLEV – endo-Levanase (Bacteroides thetaiotaomicron)
E-SUCR – Sucrase (from yeast)
E-INVRT – Invertase (fructofuranosidase) (yeast)

Megazyme endo-1,5-α-Arabinanase (Aspergillus niger)

Megazyme endo-1,5-α-Arabinanase (Aspergillus niger)

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内切-1,5- α – 阿拉伯聚糖(黑曲霉)
英文名:endo-1,5-α-Arabinanase (Aspergillus niger)
货号:E-EARAB
规格:400 Units
品牌: Megazyme

Content: 400 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: Minimum 1 year at 4oC. Check vial for details.
Enzyme Activity: endo-Arabinanase
EC Number: 3.2.1.99
CAZy Family: GH43
CAS Number: 75432-96-1
Synonyms: arabinan endo-1,5-alpha-L-arabinanase; 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase
Source: Aspergillus niger
Molecular Weight: 39,750
Concentration: Supplied at ~ 200 U/mL
Expression: From Aspergillus niger
Specificity: endo-hydrolysis of (1,5)-α-arabinofuranose linkages in (1,5)-α-arabinans. Acts more slowly on the (1,5)-α-linked arabinan backbone in branched arabinans than on linear arabinans.
Specific Activity: ~ 10 U/mg (40oC, pH 4.0 on CM-linear 1,5-α-L-arabinan)
Unit Definition: One Unit of arabinanase activity is defined as the amount of enzyme required to release one µmole of arabinose reducing-sugar equivalents per minute from CM-linear 1,5-α-L-arabinan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40oC.
Temperature Optima: 40oC
pH Optima: 4
Application examples: Applications established in food and beverage industries, particularly in the reduction of haze in fruit juices and the processing of sugar beet.

 

Megazyme E-ANAAM α-淀粉酶(米曲霉)

Megazyme E-ANAAM α-淀粉酶(米曲霉)
Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

英文名:α-Amylase (Aspergillus oryzae)
货号:E-ANAAM
规格:20,000 Units
品牌: Megazyme

alpha-Amylase Aspergillus oryzae E-ANAAM
alpha-Amylase Aspergillus oryzae E-ANAAM Scheme
Content: 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity: α-Amylase
EC Number: 3.2.1.1
CAZy Family: GH13
CAS Number: 9000-90-2
Synonyms: alpha-amylase; 4-alpha-D-glucan glucanohydrolase
Source: Aspergillus oryzae
Molecular Weight: 54,000
Concentration: Supplied at ~ 1,000 CU/mL
Expression: From Aspergillus oryzae
Specificity: endo-hydrolysis of α-1,4-D-glucosidic linkages in starch.
Specific Activity: ~ 120 U/mg (40oC, pH 5.4 on Ceralpha Reagent)
Unit Definition: One Unit of α-amylase is the amount of enzyme required to release one µmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase), pH 5.4 at 40oC, and is termed a Ceralpha Unit.
Temperature Optima: 50oC
pH Optima: 5
Application examples: For use in Megazyme Starch Damage method.

Related products   其他相关产品:
Similar Products
E-BAASS – α-Amylase (Bacillus amyloliquefaciens)
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis)
E-MAST – Malt Amylase Standard
E-MASTP – Malt Amylase Standard (powder)
Related Carbohydrates
P-AMYL – Amylose (potato)
P-BLDX-10G – β-Limit Dextrin
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
Colourimetric Substrates
K-AMYLSD – α-Amylase SD Assay Kit
K-CERA – α-Amylase Assay Kit (Ceralpha Method)
K-MALTA – Malt Amylase Assay Kit
T-AMZ-200T – Amylazyme Tablets
S-RSTAR – Red Starch
I-AZAMY – AZCL-Amylose
O-BPNPC7 – Blocked 4-nitrophenyl-α-maltoheptaoside
Associated Enzymes
E-BARBL-50KU – β-Amylase (Barley)
E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)
E-PULKP – Pullulanase M1 (Klebsiella planticola)
E-ISAMYHP – Isoamylase HP (Glycogen 6-glucanohydrolase)
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)

Megazyme 淀粉酶系列产品

Megazyme 淀粉酶系列产品

Amylase

Alpha淀粉酶[猪胰腺]

英文名:α-Amylase (Porcine Pancreatic)

货号:E-PANAA-3G

规格:3g

麦芽淀粉酶标准液

英文名:Malt Amylase Standard

货号:E-MAST

规格:100 mL; α-amylase 950 U/mL;

异淀粉酶的HP(糖原果胶6-葡聚糖水解酶)

英文名: Isoamylase HP (Glycogen 6-glucanohydrolase)

货号:E-ISAMYHP

规格:200 Units

Alpha淀粉酶[猪胰腺]

英文名: α-Amylase (Porcine Pancreatic)

货号:E-PANAA-9G

规格:9g

异支链淀粉酶(黑曲霉)

英文名:Isopullulanase (Aspergillus niger)

货号:E-ISPUAN

规格:2,000 Units at 40°C

α-淀粉酶(解淀粉芽孢杆菌)

英文名:α-Amylase (Bacillus amyloliquefaciens)

货号:E-BAASS

规格:2900 Units

Alpha淀粉酶[米曲霉]

英文名:α-Amylase (Aspergillus oryzae)

货号:E-ANAAM

规格:20000 Units

极限糊精酶和支链淀粉酶检测片剂 (200)

英文名:Limit-Dextrizyme – 200 Tablets

货号:T-LDZ-200T

规格:200 Tablets

极限糊精酶和支链淀粉酶检测片剂 (1000)

英文名:Limit-Dextrizyme – 1000 Tablets

货号:T-LDZ-1000T

规格:1000 Tablets

Alpha淀粉酶高灵敏度检测底物

英文名:Amylazyme Red – 200 Tablets

货号:T-AMZRD-200T

规格:200 Tablets

Alpha淀粉酶高灵敏度检测底物

英文名:Amylazyme Red – 1000 Tablets

货号:T-AMZRD-1000T

规格:1000 Tablets

大麦粉提取物中Alpha淀粉酶检测片剂 (200)

英文名:Amylazyme BG – 200 Tablets

货号:T-AMZBG-200T

规格:200 Tablets

Alpha淀粉酶检测底物

英文名:Amylazyme – 200 Tablets

货号:T-AMZ-200T

规格:200

Alpha淀粉酶检测底物

英文名:Amylazyme – 1000 Tablets

货号:T-AMZ-1000T

规格:1000 Tablets

Alpha淀粉酶活性检测底物

英文名:Red Starch – 5g

货号:S-RSTAR

规格:5 grams

Megazyme E-BSTAA α-淀粉酶 α-Amylase (Thermostable) (Bacillus sp.)

Megazyme E-BSTAA α-淀粉酶 α-Amylase (Thermostable) (Bacillus sp.)

上海金畔生物代理Megazyme品牌试剂产品,欢迎新老客户访问Megazyme官网或者咨询我们获取更多相关产品信息。

α-Amylase (Thermostable) (Bacillus sp.)
Ask a Scientist

High purity α-Amylase (Thermostable) (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is exceptionally thermostable and is the ideal choice for use in the total starch assay kits (K-TSTA and K-TSHK). It is considerably more thermostable than related enzymes such as the heat stable α-amylase (Bacillus licheniformis, E-BLAAM). Please see the data sheet for experimental stability data.

EC 3.2.1.1
CAZy Family: GH13
CAS: 9000-90-2 / 9000-85-5

alpha-amylase; 4-alpha-D-glucan glucanohydrolase

Highly purified. From Bacillus sp.
In 50% (v/v) glycerol plus 0.02% sodium azide
For use in Megazyme Total Starch methods, suitable for use at pH 5 and above.
Supplied at ~ 3,000 U/mL.

Specific activity:
~ 170 U/mg (40oC, pH 6.5 on Ceralpha reagent).

Stability: > 4 years below -10oC.

DESCRIPTION

α-Amylase (Thermostable) (Bacillus sp.)

EC 3.2.1.1
CAZy Family: GH13
CAS: 9000-90-2 / 9000-85-5

Synonyms:
alpha-amylase; 4-alpha-D-glucan glucanohydrolase

Form:
In 50% (v/v) glycerol plus 0.02% sodium azide

Stability:
> 4 years below -10oC.

Specific activity:
~ 170 U/mg (40oC, pH 6.5 on Ceralpha reagent).

Unit definition:.
One Unit of α-amylase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40oC.

Specificity:
Hydrolysis of α-1,4 glucosidic linkages in linear α-1,4 glucan (e.g. amylose regions in starch).

Applications:
For use in Megazyme Total Starch assay kits (K-TSTA and K-TSHK).

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Megazyme 其他活性酶Other Activities

Megazyme 其他活性酶Other Activities

Acetyl-CoA synthetase (Bacillus subtilis) 250 Units E-ACSBS
Asparaginase (E. coli) 400 Units at 25oC E-ASNEC
Catalase (Aspergillus niger) 400,000 U at 25oC E-CATLQ
Citrate synthase (E. coli) 2,500 Units E-CITEC
Diaphorase (E. coli) 1,000 Units E-DIAEC
Glucose oxidase – Catalase Mixture (eukaryote) 2 vials – 200 tests E-GOXCA
Glutamate oxaloacetate transaminase (E. coli) 5,000 Units E-GOTEC
Glutamate pyruvate transaminase (Bacillus subtilis) (Recombinant) 2,500 Units E-GPTBS
Glutaminase (E. coli) 2,500 Units E-GLUTEC
Mutase (α-Phosphoglucomutase) (microbial) 3,000 Units E-PGM
Phosphotransacetylase (Bacillus subtilis) 3,000 Units E-PTABS
Protoplast F 2.5 mL E-PROTOF
α-Rhamnosidase (prokaryote) 3,000 Units at 50oC E-RHAMS
Succinyl-CoA synthetase (prokaryote) 550 Units E-SCOAS
Trehalase (prokaryote) 8,400 Units E-TREH

Megazyme 木聚糖酶 Xylanases

Megazyme 木聚糖酶 Xylanases

endo-1,4-β-Xylanase M1 (Trichoderma viride) 8,000 Units E-XYTR1
endo-1,4-β-Xylanase M3 (Trichoderma longibrachiatum) 8,000 Units E-XYTR3
endo-1,4-β-Xylanase (Aspergillus aculeatus) 3.125 mL; 5,000 Units E-XYLAA
endo-1,4-β-Xylanase M4 (Aspergillus niger) 8,000 Units E-XYAN4
endo-1,4-β-Xylanase (rumen microorganism) 8,000 Units E-XYRU6
endo-1,4-β-Xylanase (Aeromonas punctata) 40 Units E-XYNAP
endo-1,4-β-Xylanase (Bacillus stearothermophilus T6) 2,000 Units at 70oC E-XYNBS
endo-1,4-β-Xylanase (Cellvibrio japonicus) 500 Units at 40oC E-XYNACJ
endo-1,4-β-Xylanase (Cellvibrio mixtus) 1,500 Units E-XYNBCM
endo-1,4-β-Xylanase (Neocallimastix patriciarum) 20,000 Units E-XYLNP
endo-1,4-β-Xylanase (Thermotoga maritima) 7,500 Units at 80oC E-XYLATM