EC 3.2.1.3 
CAZY Family: GH15 
From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fibre methods. Electrophoretically homogeneous. Stabilised liquid in 50% glycerol. 
Specific activity: approx. 36 U/mg (40°C, pH 4.5, soluble starch as substrate). 200 U/ml on p-nitrophenyl ß-maltoside; 3260 U/ml on soluble starch. 
Contaminants: α-amylase 4%, cellulase < 0.005%.
Stable at 4°C for > 4 years.

EC 3.2.1.3 
CAZY Family: GH15 
From A. niger. High purity. For use in Megazyme Total Starch and Dietary Fibre methods. Electrophoretically homogeneous. Stabilised liquid in 50% glycerol. 
Specific activity: approx. 100 U/mg (40°C, pH 4.5, soluble starch as substrate). 200 U/ml on p-nitrophenyl ß-maltoside; 3260 U/ml on soluble starch. 
Contaminants: α-amylase 4%, cellulase < 0.005%.
Stable at 4°C for > 4 years

EC 3.1.3.1 
Orthophosphoric-monoester phosphohydrolase (alkaline optimum). 
Recombinant from E. coli. In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4.
Specific activity: ~ 10 U/mg (37oC, pH 10.4, 4-Nitrophenyl phosphate). 
Stable at 4oC for > 2 years.a

EC 4.2.2.3 CAZY Family: PL7 Recombinant. From Sphingomonas sp. In 3.2 M ammonium sulphate. 

Endo-acting β-elimination cleavage of the polysaccharide, alginate. 

Specific activity: ~ 120 U/mg (40°C, pH 7.2 on sodium alginate).

 Store at 4°C. 

EC 3.2.1.22 CAZY Family: GH27 From guar seed. 

Highly purified. Stabilised with BSA. In 3.2 M ammonium sulphate. 

Specific activity: 65 U/mg (40°C, pH 4.5, p-nitrophenyl α-D-galactopyranoside, before addition of BSA). 

Contaminants: -mannanase and -mannosidase < 0.00001%. 

This enzyme is very active on galactomannans. 

Stable at 4°C for > 4 years.

EC 3.2.1.22 CAZY Family: GH36 

From A. niger. Highly purified. 

In 3.2 M ammonium sulphate. 

Specific activity: >1020 U/mg (40°C, pH 4.5, p-nitrophenyl α-D-galactopyranoside.

Contaminants: β-mannanase and β-mannosidase < 0.001%. This enzyme is very active on raffinose, stachyose and verbascose. 

Stable at 4°C for > 4 years.a

EC 3.2.1.55 CAZY Family: GH51 From A. niger. In 3.2 M ammonium sulphate. 

Specific activity: 19 U/mg (40°C, pH 4.0, p-nitrophenyl α-L-arabinofuranoside); 7.5 U/mg on sugar-beet arabinan; 0.7 U/mg on wheat arabinoxylan (10 mg/ml). 

Contaminants: endo-arabinanase and endo-galactanase < 0.001%; endo-xylanase < 0.01%. Stable at 4°C for > 4 years.

EC 3.2.1.55 CAZy Family: GH43 From Bifidobacterium adolescentis. In 3.2 M ammonium sulphate. 

Specific activity: ~ 90 U/mg on xylanase degraded wheat arabinoxylan (at 10 mg/mL, 40°C, pH 6.0); ~ 28 U/mg on wheat arabinoxylan (at 10 mg/mL, 40°C, pH 6.0); ~ 4 U/mg on sugar-beet arabinan; ~ 0.1 U/mg on p-nitrophenyl-α-L-arabinofuranoside. 

Contaminants: endo-arabinanase and endo-galactanase < 0.0001 %. 

Stable at 4°C for > 4 years.

EC 1.1.1.1 Recombinant from E. coli. In 3.2 M ammonium sulphate. 

Specific activity: ~ 10 U/mg (25oC, pH 8.5). 

Contaminants: lactate dehydrogenase < 0.01 %, myokinase < 0.01 %, NADH oxidase < 0.001 %. 

Low Km for ethanol: due to the low Km of this enzyme, only 3 units are required per assay (TV = 2.54 mL) compared to 177 units of the yeast enzyme. 

EC 6.2.1.1 From B. subtilis. In 3.2 M ammonium sulphate. 

Specific activity: 7.6 U/mg (37°C, pH 7.5); 39 U/mg (37°C, pH 8.4); 7.0 U/mg (25°C, pH 7.5). 

Stable at 4°C for > 2 years.