Megazyme K-RAFGL 密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒

Megazyme K-RAFGL 密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒
Megazyme试剂盒代理、Megazyme代理
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中文名称:密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒
英文名:Raffinose/Sucrose/D-Glucose Assay Kit
货号:K-RAFGL
规格:120 assays of each per kit
品牌: Megazyme

The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Colourimetric method for the determination of Raffinose (also 
stachyose and verbascose), Sucrose and D-Glucose in 
legume seeds, plant materials, foodstuffs and feed

棉子糖/蔗糖/D-葡萄糖检测试剂盒用于测量和分析种子和种子粉中的 D-葡萄糖、蔗糖和棉子糖、水苏糖和毛蕊花糖。 基于 D-葡萄糖对棉子糖、水苏糖和毛蕊花酶水解为 D-葡萄糖、D-果糖和 D-半乳糖的测量。

比色法测定棉子糖(也
水苏糖和毛蕊花)、蔗糖和 D-葡萄糖
豆类种子、植物材料、食品和饲料

Principle:
(α-galactosidase)
(1) Raffinose + stachyose + verbascose + H2O → D-galactose + 
sucrose

(invertase)
(2) Sucrose + H2O → D-glucose + D-fructose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
quinoneimine + 4H2O

Kit size: 120 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Application examples:
Analysis of grain legumes and other materials containing raffinose,
stachyose and verbascose
Method recognition: Used and accepted in food analysis

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme . Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q1。即使对于酸性介质中的样品,也应调整样品的 pH 值吗?

添加样品后检测溶液的 pH 值应与试剂盒随附的检测缓冲液的 pH 值相同。
低样品量(例如 0.1 mL)不太可能影响分析溶液的 pH 值,因此可能不需要调整 pH 值。
超过 0.1 mL 的样品更有可能影响分析溶液的 pH 值,因此在添加到分析之前,应按照数据手册中的说明调整这些样品的 pH 值。

Q2。套件的性能存在问题;结果并不如预期。

如果您怀疑 Megazyme 检测试剂盒未按预期运行,从而无法获得预期结果,请执行以下操作:

确保您已经测试了 Megazyme 测试套件随附的标准样品。
将相关 MegaCalc 电子表格(如果有)中的试剂盒标准品、空白样品和为您的样品获得的结果发送给 Megazyme。相关的 MegaCalc 电子表格可以从 Megazyme 网站上产品出现的地方下载。
说明正在使用的套件批号(可在套件盒的外面找到)。
说明使用了哪种检测格式(如有必要,请参阅试剂盒手册中的相关页面)。
说明对 Megazyme 提供的标准程序的任何修改的确切细节。
说明样品类型并描述样品制备步骤(如果适用)。

Megazyme E-FRMXPD 果聚糖酶混合物

Megazyme E-FRMXPD 果聚糖酶混合物

Product code: E-FRMXPD
20,000 Units

Content: 20,000 Units
Shipping Temperature: Ambient
Storage Temperature: Below -10oC
Formulation: Supplied as a lyophilised powder
Physical Form: Powder
Stability: Minimum 1 year at < -10oC. Check vial for details.
Enzyme Activity: Fructanase
EC Number: endo-Inulinase: 3.2.1.7,
exo-Inulinase: 3.2.1.80
CAZy Family: GH32
CAS Number: 9001-57-4,
9025-67-6,
37288-56-5
Synonyms: endo-inulinase: 1-beta-D-fructan fructanohydrolase
exo-inulinase: fructan β-fructosidase
Source: Aspergillus sp.
Expression: From Aspergillus sp.
Specificity: endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin.
exo-Inulinase: Hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides.
Unit Definition: endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5.
exo-Inulinase: One Unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4.5 at 40oC.
Temperature Optima: 40oC
pH Optima: 4.5
Method recognition: AOAC Method 997.08, AOAC Method 2000.11, AOAC Method 2016.06, GB Standard 5009.245-2016 and GB Standard 5009.255-2016

Powder (freeze dried)

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.

Componentsexo-inulinase 20,000 U (on kestose, at 40oC), endo-inulinase 1000 U (on fructan at 40oC), α-galactosidase 0.70 U (on p-nitrophenyl α-galactoside at 40oC), β-Glucanase 1.25 U (on β-glucan at 40oC) and pectinase 5.5 U (on pectin at 40oC).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).

Fructanase and sucrase (E-SUCR) are used in the enzyme hydrolysis step of the AOAC method 2016.06 and the Chinese GB Standard 5009.255-2016 for the determination of fructan in foods.

See more of our other Carbohydrate Active enZYmes products.

Validation of Methods

内容:20,000个单位
运输温度:环境
储存温度:-10oC以下
配方:以冻干粉形式提供
物理形态:粉末
稳定性:在 < -10oC 时至少 1 年。检查小瓶了解详情。
酶活性:果聚糖酶
EC编号:菊粉内切酶:3.2.1.7,
外切菊糖酶:3.2.1.80
CAZy 家族:GH32
CAS 编号:9001-57-4,
9025-67-6,
37288-56-5
同义词:菊粉内切酶:1-β-D-果聚糖果糖水解酶
外切菊糖酶:果聚糖β-果糖苷酶
来源:曲霉属。
表达:来自Aspergillus sp。
特异性:菊粉内切酶:菊粉中 (2,1)-β-D-果糖苷键的内切作用水解。
外切菊糖酶:水解 β-D-呋喃果糖苷中末端的非还原性 β-D-呋喃果糖苷残基。
单位定义:菊粉内切酶:一个菊糖内切酶活性单位定义为在乙酸钠缓冲液中每分钟从菊粉 (20 mg/mL) 中释放 1 μmole β-D-果糖还原糖当量所需的酶量(100 毫米), pH 值 4.5。
外切菊糖酶:一单位外切菊糖酶活性定义为在乙酸钠缓冲液(100 mM)中每分钟从酮糖 (5 mg/mL) 中释放 1 μmole β-D-果糖还原糖当量所需的酶量),在 40oC 时 pH 值 4.5。
最佳温度:40oC
最佳 pH 值:4.5
方法识别:AOAC Method 997.08、AOAC Method 2000.11、AOAC Method 2016.06、GB Standard 5009.245-2016和GB Standard 5009.255-2016
粉末(冻干)

果聚糖酶、淀粉葡萄糖苷酶 (E-AMGDF) 和异淀粉酶 (E-ISAMY) 用于两种经过验证的食品中聚葡萄糖(一种低摩尔质量膳食纤维)测定方法的酶水解步骤:AOAC 方法 2000.11 和中国 GB 标准 5009.245 -2016 年。

成分:菊糖外切酶 20,000 U(在 40oC 条件下对 kestose),菊粉内切酶 1000 U(在 40oC 条件下对果聚糖),α-半乳糖苷酶 0.70 U(在 40oC 条件下对硝基苯基α-半乳糖苷),β-葡聚糖酶 1.25 U( β-葡聚糖,40oC)和果胶酶 5.5 U(果胶,40oC)。

注意:本产品已经过纯化,去除了干扰制剂在果聚糖测定(AOAC 方法 997.08)和聚葡萄糖测定(AOAC 方法 2000.11)中使用的 α-半乳糖苷酶、β-葡聚糖酶和果胶酶。

果聚糖酶和蔗糖酶 (E-SUCR) 用于 AOAC 方法 2016.06 和中国 GB 标准 5009.255-2016 测定食品中果聚糖的酶解步骤。

查看更多我们的其他碳水化合物活性酶产品。

Related products  相关产品:
Similar Products
E-FRMXLQ – Fructanase Mixture (liquid)
E-EXOIAN – exo-Inulinase (Aspergillus niger)
E-ENDOIAN – endo-Inulinase (Aspergillus niger)

Related Carbohydrates
P-INUL – Inulin
P-FOS28 – Fructooligosaccharides (FOS)
O-KTR – 1-Kestose
O-KTE – 1,1-Kestotetraose
O-KPE – 1,1,1-Kestopentaose

Colourimetric Substrates
S-AZFR5 – Azo-Fructan
S-AZFRXOI – Azo-Fructan

Associated Enzymes
E-INVPD-2G – Invertase
E-ENLEV – endo-Levanase (Bacteroides thetaiotaomicron)
E-SUCR – Sucrase (from yeast)
E-INVRT – Invertase (fructofuranosidase) (yeast)