Megazyme K-RAFGL 密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒

Megazyme K-RAFGL 密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

中文名称:密三糖或棉子糖/蔗糖/D-半乳糖检测试剂盒
英文名:Raffinose/Sucrose/D-Glucose Assay Kit
货号:K-RAFGL
规格:120 assays of each per kit
品牌: Megazyme

The Raffinose/Sucrose/D-Glucose test kit is for the measurement and analysis of D-glucose, sucrose and raffinose, stachyose and verbascose in seeds and seed meals. Based on the measurement of D-glucose on enzymic hydrolysis of raffinose, stachyose and verbascose to D-glucose, D-fructose and D-galactose.

Colourimetric method for the determination of Raffinose (also 
stachyose and verbascose), Sucrose and D-Glucose in 
legume seeds, plant materials, foodstuffs and feed

棉子糖/蔗糖/D-葡萄糖检测试剂盒用于测量和分析种子和种子粉中的 D-葡萄糖、蔗糖和棉子糖、水苏糖和毛蕊花糖。 基于 D-葡萄糖对棉子糖、水苏糖和毛蕊花酶水解为 D-葡萄糖、D-果糖和 D-半乳糖的测量。

比色法测定棉子糖(也
水苏糖和毛蕊花)、蔗糖和 D-葡萄糖
豆类种子、植物材料、食品和饲料

Principle:
(α-galactosidase)
(1) Raffinose + stachyose + verbascose + H2O → D-galactose + 
sucrose

(invertase)
(2) Sucrose + H2O → D-glucose + D-fructose

(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
quinoneimine + 4H2O

Kit size: 120 assays
Method: Spectrophotometric at 510 nm
Reaction time: ~ 20 min
Detection limit: 100 mg/L
Application examples:
Analysis of grain legumes and other materials containing raffinose,
stachyose and verbascose
Method recognition: Used and accepted in food analysis

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

Q1. Should the pH of the sample be adjusted even for samples in acidic media?

The pH of the assay solution after the sample is added should be the same as that of the assay buffer that is supplied with the kit.
Low sample volumes (e.g. 0.1 mL) are not likely to affect the pH of the assay solution and therefore may not require pH adjustment.
Samples above 0.1 mL are more likely to affect the pH of the assay solution and therefore the pH of these samples should be adjusted as described in the data booklet, prior to addition to the assay.

Q2. There is an issue with the performance of the kit; the results are not as expected.

If you suspect that the Megazyme test kit is not performing as expected such that expected results are not obtained please do the following:

  1. Ensure that you have tested the standard sample that is supplied with the Megazyme test kit.
  2. Send the results of the kit standard, blank samples and the results obtained for your sample, in the relevant MegaCalc spreadsheet (if available) to Megazyme . Where available the relevant MegaCalc spreadsheet can be downloaded from where the product appears on the Megazyme website.
  3. State the kit lot number being used (this is found on the outside of the kit box).
  4. State which assay format was used (refer to the relevant page in the kit booklet if necessary).
  5. State exact details of any modifications to the standard procedure that is provided by Megazyme.
  6. State the sample type and describe the sample preparation steps if applicable.

Q1。即使对于酸性介质中的样品,也应调整样品的 pH 值吗?

添加样品后检测溶液的 pH 值应与试剂盒随附的检测缓冲液的 pH 值相同。
低样品量(例如 0.1 mL)不太可能影响分析溶液的 pH 值,因此可能不需要调整 pH 值。
超过 0.1 mL 的样品更有可能影响分析溶液的 pH 值,因此在添加到分析之前,应按照数据手册中的说明调整这些样品的 pH 值。

Q2。套件的性能存在问题;结果并不如预期。

如果您怀疑 Megazyme 检测试剂盒未按预期运行,从而无法获得预期结果,请执行以下操作:

确保您已经测试了 Megazyme 测试套件随附的标准样品。
将相关 MegaCalc 电子表格(如果有)中的试剂盒标准品、空白样品和为您的样品获得的结果发送给 Megazyme。相关的 MegaCalc 电子表格可以从 Megazyme 网站上产品出现的地方下载。
说明正在使用的套件批号(可在套件盒的外面找到)。
说明使用了哪种检测格式(如有必要,请参阅试剂盒手册中的相关页面)。
说明对 Megazyme 提供的标准程序的任何修改的确切细节。
说明样品类型并描述样品制备步骤(如果适用)。

Megazyme P-PACHY Pachyman (1,3-β-D-Glucan)

Megazyme P-PACHY Pachyman (1,3-β-D-Glucan)
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

 This product is currently unavailable, please contact

Pachyman 1-3-beta-D-Glucan P-PACHY
Content: 5 g
Shipping Temperature: Ambient
Storage Temperature: Ambient
Physical Form: Powder
Stability: > 10 years under recommended storage conditions
CAS Number: 9037-88-1
Source: Poria Coco
Purity: > 98%
Monosaccharides (%): D-glucose = 98
Main Chain Glycosidic Linkage: β-1,3
Substrate For (Enzyme): endo-1,3-β-Glucanase

This product has been discontinued (read more).

High purity Pachyman (1,3-β-D-Glucan) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For the assay of endo-1,3-β-D-glucanase.

Other carbohydrate products also offered.

Megazyme K-ACHYD 乙醛检测试剂盒

Megazyme K-ACHYD 乙醛检测试剂盒
Megazyme试剂盒
Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

乙醛检测试剂盒
英文名:Acetaldehyde Assay Kit
货号:K-ACHYD
规格:50 assays (manual)
品牌: Megazyme

Advantages
  • Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
  • No wasted aldehyde dehydrogenase solution (stable suspension supplied)
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats
优势
  • 扩展协因子稳定性溶解辅助性因子在4时稳定>1年oC.
  • 无废醛脱氢酶溶液(提供稳定悬浮液)
  • 极具竞争力的价格(每次测试成本)
  • 所有试剂在制备后均稳定>2年。
  • 简单格式
  • Mega-calc™软件工具可从我们的网站获得无麻烦的原始数据处理。
  • 标准包括
  • 适用于手动、微型板和自动分析仪格式。
Description
Content: 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Acetaldehyde
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 0.5 to 20 µg of acetaldehyde per assay
Limit of Detection: 0.18 mg/L
Reaction Time (min): ~ 4 min
Application examples: Wine, champagne, beer, liqueurs, brandy, dairy products (e.g. yogurt), bread, fruit juices, soft drinks, cocoa, vegetable and fruit products, coffee, and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Methods based on this principle have been accepted by MEBAK

The Acetaldehyde test kit is a simple, reliable and accurate method for the measurement and analysis of acetaldehyde in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

Scheme-K-ACHYD K-ACHYD Megazyme

内容: 50项试验(手册)/500项试验(微板)/500项试验(自动分析仪)
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 乙醛
化验格式: 分光光度计,微板,自动分析仪
检测方法: 吸光度
波长(纳米): 340
信号响应: 增加
线性范围: 0.5至20克乙醛每次测定
检测极限: 0.18毫克/升
反应时间(最低): ~4分钟
应用实例: 葡萄酒、香槟、啤酒、利口酒、白兰地、乳制品(如酸奶)、面包、果汁、软饮料、可可、蔬菜和水果制品、咖啡和其他材料(例如生物培养物、样品等)。
方法识别: 基于这一原则的方法已被MEBAK所接受。

乙醛检测试剂盒是测定和分析饮料和食品中乙醛的一种简便、可靠和准确的方法。

内容说明:如果将所有卷减少一半,每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).

Acetaldehyde Kit (K-ACHYD)
When using the K-ACHYD Assay Kit for quantitative analysis what level of accuracy and repeatability can be expected?
Thu, 10 May, 2018 at 3:09 PM

What is the detection limit of the K-ACHYD Assay Kit?
Thu, 10 May, 2018 at 3:09 PM
What is the linear range of the K-ACHYD Assay Kit?
Thu, 10 May, 2018 at 3:09 PM
The standard solution supplied with the K-ACHYD Assay Kit is acetaldehyde ammonia powder. Can I use this as an internal standard?
Thu, 10 May, 2018 at 3:10 PM
Will ethanol affect the acetaldehyde kit (K-ACHYD) assay?
Thu, 10 May, 2018 at 3:10 PM
How are mg of acetaldehyde calculated from mg of acetaldehyde ammonia trimer in the Acetaldehyde Assay Kit (K-ACHYD)?
Thu, 10 May, 2018 at 3:10 PM

乙醛试剂盒
当使用K-ACHYD分析试剂盒进行定量分析时,可以期望有多高的准确度和重复性?

K-ACHYD试剂盒的检出限是多少?

K-ACHYD试剂盒的线性范围是多少?

用K-ACHYD试剂盒提供的标准溶液是乙醛氨粉.我能用这个作为内部标准吗?

乙醇会影响乙醛试剂盒(K-ACHYD)的测定吗?

乙醛试剂盒(K-ACHYD)中乙醛氨三聚体Mg是如何计算的?

图片仅供参考,请以实物为准。
若本网站没有及时更新,请大家谅解!
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

Megazyme 淀粉酶检测试剂盒 Amylase Assay Kit

Megazyme 淀粉酶检测试剂盒 Amylase Assay Kit
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

麦芽淀粉酶检测试剂盒 Malt Amylase Assay Kit
β-淀粉酶检测试剂盒 β-Amylase Assay Kit (Betamyl-3)
α-淀粉酶/液化酶/淀粉糖化酶检测试剂盒 α–Amylase Assay Kit

Megazyme K-ETOH 乙醇检测试剂盒说明书

Megazyme K-ETOH 乙醇检测试剂盒说明书
Megazyme试剂盒
Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
Megazyme K-ETOH 乙醇检测试剂盒
中文名称:乙醇检测试剂盒
英文名:Ethanol Assay Kit
货号:K-ETOH
规格:60 assays (manual)

The Ethanol test kit is a simple, reliable and accurate method for the measurement and analysis of ethanol in beverages and foodstuffs.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Ethanol in foodstuffs, beverages, and other materials

Principle原理:
(alcohol dehydrogenase)
(1) Ethanol + NAD+ ↔ acetaldehyde + NADH + H+

(aldehyde dehydrogenase)
(2) Acetaldehyde + NAD+ + H2O → acetic acid + NADH + H+

Kit size: 60 assays (manual) / 600 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.093 mg/L
Application examples:
Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic
/ non-alcoholic beverages, pickles, fruit and fruit juice, chocolate
products, vinegar, jam, bread and bakery products, honey, soy sauce,
dairy products, cosmetics, pharmaceuticals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by IFU, EBC,
MEBAK and ASBC

Advantages 优点:

Simple format – aldehyde dehydrogenase supplied as stable suspension

Very competitive price (cost per test)

All reagents stable for > 2 years after preparation

Rapid reaction

Mega-Calc™ software tool is available from our website for hassle-free raw data processing

Standard included

Extended cofactors stability

Suitable for manual, microplate and auto-analyser formats

Content: 60 assays (manual) / 600 assays (microplate) / 600 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Ethanol
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 0.25 to 12 µg of ethanol per assay
Limit of Detection: 0.093 mg/L
Reaction Time (min): ~ 5 min
Application examples: Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic / non-alcoholic beverages, pickles, fruit and fruit juice, chocolate products, vinegar, jam, bread and bakery products, honey, soy sauce, dairy products, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Methods based on this principle have been accepted by AOAC (AOAC Method 2019.08, First Action), IFU, EBC, MEBAK and ASBC

The Ethanol test kit is a simple, reliable and accurate method for the measurement and analysis of ethanol in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).

内容: 60项试验(手册)/600项试验(微板)/600项试验(自动分析仪)
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 乙醇
化验格式: 分光光度计,微板,自动分析仪
检测方法: 吸光度
波长(纳米): 340
信号响应: 增加
线性范围: 每次测定乙醇0.25至12微克
检测极限: 0.093毫克/升
反应时间(最低): ~5分钟
应用实例: 葡萄酒、啤酒、苹果酒、含酒精果汁、烈酒、利口酒、低酒精/不含酒精饮料、泡菜、水果和果汁、巧克力产品、醋、果酱、面包和面包店产品、蜂蜜、酱油、奶制品、化妆品、药品和其他材料(如生物培养物、样品等)。
方法识别: 基于这一原则的方法已被aaac(aac方法2019.08,First Action)、ifu、ebc、mebak和asbc所接受。

乙醇检测试剂盒是测定和分析饮料和食品中乙醇的一种简便、可靠和准确的方法。

内容说明:如果将所有卷减少一半,每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).

Scheme-K-ETOH ETOH megayzme

Related Products
K-ETOHLQR – Ethanol Assay Kit (Liquid Ready) Assay Kit
K-LMAL-116A – L-Malic Acid Assay Kit (Manual Format)
K-ACETRM – Acetic Acid Assay Kit (Acetate Kinase Manual Format)
K-FRUGL – D-Fructose/D-Glucose Assay Kit
K-LARGE – L-Arginine/Urea/Ammonia Assay Kit
Related Equipment
D-CHEM2910 – ChemWell® 2910 Automated EIA and Chemistry Analyzer
D-CHEMT – ChemWell®-T Automated Chemistry Analyzer
D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer)

Megazyme K-RSTCL 抗性淀粉试剂盒对照淀粉标品

Megazyme K-RSTCL 抗性淀粉试剂盒对照淀粉标品
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

英文名:Resistant Starch Control Flours
货号:K-RSTCL
规格:5 Controls per kit
品牌: Megazyme

Content: 5 Controls per kit: Use with K-RSTAR
Shipping Temperature: Ambient
Storage Temperature: Short term stability: Ambient,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Resistant Starch

For use with the Resistant Starch assay kit.

内容: 每个工具包5个控件:与K-RSTAR一起使用
船运温度: 环境
储存温度: 短期稳定性:环境,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 抗性淀粉

用于抗性淀粉检测试剂盒。

Scheme-K-RSTCL RSTCL Megazyme

Related products
Similar Products
K-RAPRS – Resistant Starch Assay Kit (Rapid)
K-RSTAR – Resistant Starch Assay Kit
K-TSTA-100A – Total Starch Assay Kit (AA/AMG)
K-TSHK – Total Starch HK Assay Kit
K-TSCK – Total Starch Control Flours
K-SDAM – Starch Damage Assay Kit
Related Carbohydrates
P-AMYL – Amylose (potato)
P-BLDX-10G – β-Limit Dextrin
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
Colourimetric Substrates
K-CERA – α-Amylase Assay Kit (Ceralpha Method)
T-AMZ-200T – Amylazyme Tablets
S-RSTAR – Red Starch
I-AZAMY – AZCL-Amylose
Associated Enzymes
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis)
E-BARBL-50KU – β-Amylase (Barley)
E-PULKP – Pullulanase M1 (Klebsiella planticola)
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
E-TSAGS – α-Glucosidase (Bacillus stearothermophilus) (Recombinant)

 

Megazyme K-CERA α-Amylase Assay Kit α-淀粉酶检测试剂盒

Megazyme K-CERA α-Amylase Assay Kit α-淀粉酶检测试剂盒

α-Amylase Assay Kit (Ceralpha Method)

上海金畔生物代理Megazyme品牌试剂产品,欢迎新老客户访问Megazyme官网或者咨询我们获取更多相关产品信息。
图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

Content: 100 / 200 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: α-Amylase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: 0.05 U/mL
Reproducibility (%): ~ 3%
Total Assay Time: ~ 30 min
Application examples: Cereal flours, fermentation broths and other materials.
Method recognition: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018)
内容: 每套试剂盒100/200次化验
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: α-淀粉酶
化验格式: 分光光度计,自动分析仪
检测方法: 吸光度
波长(纳米): 400
信号响应: 增加
检测极限: 0.05U/mL
可重复性(%): ~ 3%
试验总时间: ~30分钟
应用实例: 谷物粉,发酵液和其他材料。
方法识别: AACC方法22-02.01,aac方法2002.01,ICC标准编号303,RACI标准方法和CCFRA(面粉测试工作组方法0018)

The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).

α-淀粉酶检测试剂盒适用于谷物和发酵液(真菌和细菌)中α-淀粉酶的特异性测定和分析。

Browse the complete list of our enzyme activity assay kits.

Scheme-K-CERA CERA Megazyme

Related products  其他相关产品:
Similar Products
K-AMYLSD – α-Amylase SD Assay Kit
K-MALTA – Malt Amylase Assay Kit
Related Carbohydrates
P-AMYL – Amylose (potato)
P-BLDX-10G – β-Limit Dextrin
P-GLYAL – Glycogen (Algae)
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
Colourimetric Substrates
R-CAAR4 – α-Amylase Reagent (Ceralpha)
R-AMHR4 – α-Amylase HR Reagent
O-BPNPC7 – Blocked 4-nitrophenyl-α-maltoheptaoside
S-RSTAR – Red Starch
I-AZAMY – AZCL-Amylose
Associated Enzymes
E-ANAAM – α-Amylase (Aspergillus oryzae)
E-BAASS – α-Amylase (Bacillus amyloliquefaciens)
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis)
E-BSTAA – α-Amylase (Thermostable) (Bacillus sp.)
E-MAST – Malt Amylase Standard

Megazyme K-FRHYD 甲醛检测试剂盒

Megazyme K-FRHYD 甲醛检测试剂盒
英文名:Formaldehyde Assay Kit
货号:K-FRHYD
规格:50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
品牌: Megazyme

The Histamine Assay Kit provides a simple robust method for the measurement of histamine.

Content: 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Formaldehyde
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 0.1 to 14 μg of formaldehyde per assay
Limit of Detection: 0.033 mg of formaldehyde per test or
0.016 mg/mL of formaldehyde in a sample treated as per the standard procedure
Limit of Quantification: 0.11 mg of formaldehyde per test or
0.054 mg/mL of formaldehyde in a sample treated as per the standard procedure
Reproducibility (%): ~ 3%
Reaction Time (min): ~ 15 min
Application examples: Environmental samples, food and beverage samples.

组胺分析试剂盒为组胺的测定提供了一种简单、可靠的方法。

内容: 50项试验(手册)/500项试验(微板)/500项试验(自动分析仪)
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 甲醛
化验格式: 分光光度计,微板,自动分析仪
检测方法: 吸光度
波长(纳米): 340
信号响应: 增加
线性范围: 每次测定甲醛0.1~14μg
检测极限: 每次试验甲醛0.033毫克
按标准程序处理的样品中甲醛含量为0.016毫克/毫升
数量限制: 每次试验甲醛0.11毫克
按标准程序处理的样品中甲醛含量为0.054毫克/毫升
可重复性(%): ~ 3%
反应时间(最低): ~15分钟
应用实例: 环境样品,食品和饮料样品。

Megazyme代理 K-TDFR 膳食纤维总量检测试剂盒中文说明书

Megazyme代理 K-TDFR 膳食纤维总量检测试剂盒中文说明书

膳食纤维总量检测试剂盒
英文名:Total Dietary Fibre Assay Kit
货号:K-TDFR-200A
规格:200 assays per kit
Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

膳食纤维控粉总量检测试剂盒
综合总膳食纤维检测试剂盒
总膳食纤维快速整合试剂盒
膳食纤维总量检测试剂盒

Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol
precipitation

(4) Ash and residual protein determined on DF residues
and subtracted

Megazyme代理 K-TDFR 膳食纤维总量检测试剂盒中文说明书

货号 规格
K-TDFR-100A

 

100分析
K-TDFR-200A

 

200测定

原理: (α淀粉酶+淀粉葡糖苷酶)
(1)淀粉+ H 2 O→d-葡萄糖

(蛋白酶)
(2)蛋白+ H 2 O→肽
(3)膳食纤维在酒精沉淀之后通过重量分析确定
(4)在DF残留物上测定灰分和残余蛋白并减去

优点

  • 极具竞争力的价格(每次测试成本)
  • 所有试剂稳定2年以上
  • 采用高纯度/标准化酶
  • Mega-Calc™软件工具可从我们的上获取,以获得无忧的原始数据处理
  • 简单的格式

Total Dietary Fiber Assay Kit,根据AOAC和AACC批准的方法测量和分析总,可溶性和不溶性膳食纤维。参见总裁判报告:AOAC INTERNATIONAL杂志,Vol。81,No.1,1998。

纤维是复​​杂有机物质的混合物,包括亲水性化合物,例如可溶性和不可溶性多糖和不可消化的低聚糖,以及一系列不可溶胀的或多或少疏水性的化合物,如角质素,软木脂和木质素。我们的小册子中概述的总膳食纤维测定和分析程序是基于Lee 等人的方法。1 和普罗斯盖等。2,3  (AOAC 991.43,AOAC 985.29,AACC 32-07.01和AACC 32-05.01)。然而,Megazyme总膳食纤维套件中的酶也可用于其他膳食纤维分析方法,如AACC方法32-21.01和AACC方法32-06.01。

1.分析化学家协会。(1985)。Official Methods of Analysis,14th ed。,1st suppl。秒。43,A14-43,A20,p.399。

2.分析化学家协会。(1986年)。方法的变化。J. Assoc。关闭。肛门。化学。,69,370。

相关数据手册中介绍了两种独立的方法:

方法1:

测定总量,可溶性和不溶性膳食纤维

基于AOAC方法991.43“食品中总量,可溶性和不溶性膳食纤维”(First Action 1991)和AACC Method 32-07.01“食品和食品中可溶性,不溶性和总膳食纤维的测定”(zui终批准10- 16-91)。

方法2:总膳食纤维的测定

Content: 100 assays / 200 assays
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Dietary Fiber
Assay Format: Enzymatic
Detection Method: Gravimetric
Signal Response: Increase
Limit of Detection: 0.5 g/100 g
Total Assay Time: ~ 100 min
Application examples: Food ingredients, food products and other materials.
Method recognition: AACC Method 32-05.01, AACC Method 32-06.01, AACC Method 32-07.01, AACC Method 32-21.01, AOAC Method 985.29, AOAC Method 991.42, AOAC Method 991.43, AOAC Method 993.19, CODEX Method Type I and GB Standards 5009.88-2014

The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods.

Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.

Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.

Two separate methods are described in the associated assay protocol:

METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER
Based on AACC method 32-05.01 and AOAC Method 985.29.

Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.

内容: 100次/200次
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 膳食纤维
化验格式: 酶学
检测方法: 重量法
信号响应: 增加
检测极限: 0.5克/100克
试验总时间: ~100分钟
应用实例: 食品配料、食品及其他材料。
方法识别: AACC方法32-05.01,AACC方法32-06.01,AACC方法32-07.01,AACC方法32-21.01,aac方法985.29,aac方法991.42,aac方法991.43,aac方法993.19,Codex方法I和GB标准5009.88-2014。

总膳食纤维分析试剂盒对总膳食纤维、可溶性和不溶性膳食纤维的分析 根据AOAC和AACC批准的方法。

膳食纤维一般可以被描述为食物中碳水化合物的含量,而这些碳水化合物在人类小肠中是不被消化的。它通过进入大肠,在那里它是部分或完全发酵。膳食纤维的这些特性与其大量的健康益处有着密切的关系。

膳食纤维是一种复杂的有机物质的混合物,包括水溶性和不溶性多糖和不可消化的低聚糖等亲水化合物,以及一系列不可膨胀、或多或少疏水的化合物,如木质素、木质素和木质素。测定和分析总膳食纤维的方法是基于Lee的方法。等人.1普洛斯基等人.2,3(AOAC 991.43,AOAC 985.29,AACC 32-07.01和AACC 32-05.01)。然而,大麦草总膳食纤维试剂盒中的酶也可用于其他膳食纤维分析方法,如AACC法32-21.01和AACC法32-06.01。

1.官方分析化验师协会。(1985年)。官方分析方法,第14版,第一补编。赛斯。43,A14-43,A20,第399页。
2.官方分析化验师协会。(1986年)。方法的改变。J.Assoc.关掉。肛门。化学。, 69, 370.
3.官方分析化验师协会。(1987年)。方法的改变。J.Assoc.关掉。肛门。化学., 70, 393.

见总裁判报告:AOAC国际杂志,第81卷,第1号,1998年。

在相关的检测协议中描述了两种不同的方法:

方法1:总膳食纤维、可溶性膳食纤维和不溶性膳食纤维的测定
根据美国aac方法991.43“食品中的总、可溶性和不溶性膳食纤维”(1991年第一行动)和AACC方法32-07.01“食品和食品中可溶性、不溶性和总膳食纤维的测定”(最终批准10-16-91)。

方法2:膳食纤维总量的测定
基于AACC方法32-05.01和AOAC方法985.29.

请注意,原始方法开发人员LeonProsky博士的背书包含在DocumentsTab中。

Scheme-K-TDFR-200A TDFR Megazyme

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Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
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