Description Documents (37) Related Products Publications (3) Safety Information
Content:
7,000 Units in 50% aqueous glycerol
Shipping Temperature:
Ambient
Storage Temperature:
Below -10oC
Formulation:
In 50% (v/v) glycerol
Physical Form:
Solution
Stability:
> 4 years at 4oC or below -10oC
Enzyme Activity:
Pectate Lyase
EC Number:
4.2.2.2
CAZy Family:
PL1
CAS Number:
9015-75-2
Synonyms:
pectate lyase; (1→4)-alpha-D-galacturonan lyase
Source:
Aspergillus sp.
Molecular Weight:
45,000
Concentration:
Supplied at ~ 1,400 U/mL
Expression:
Purified from Aspergillus sp.
Specificity:
Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.
Specific Activity:
~ 180 U/mg (40oC, pH 8.0 on polygalacturonic acid)
Unit Definition:
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of galacturonic acid from polygalacturonic acid (2.5 mg/mL) per min in Tris.HCl buffer (50 mM), pH 8.0 at 40oC.
Temperature Optima:
55oC
pH Optima:
8
Application examples:
Suitable for pectin identification according to USP method [Pectin 9000-69-5]; Pectin Identification Procedure. Applications in carbohydrate and biofuels research.
High purity Pectate lyase (Aspergillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
More Carbohydrate Active enZYmes on our products list.