Megazyme K-CERA α-Amylase Assay Kit α-淀粉酶检测试剂盒

Megazyme K-CERA α-Amylase Assay Kit α-淀粉酶检测试剂盒

α-Amylase Assay Kit (Ceralpha Method)

上海金畔生物代理Megazyme品牌试剂产品,欢迎新老客户访问Megazyme官网或者咨询我们获取更多相关产品信息。
图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究,不能作为”药品”,”食品”,”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品(包括但不限于抗生素类、蛋白质类、试剂盒类产品等)仅限用于科学研究用途,不得作用于人体。

Content: 100 / 200 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: α-Amylase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: 0.05 U/mL
Reproducibility (%): ~ 3%
Total Assay Time: ~ 30 min
Application examples: Cereal flours, fermentation broths and other materials.
Method recognition: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018)
内容: 每套试剂盒100/200次化验
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: α-淀粉酶
化验格式: 分光光度计,自动分析仪
检测方法: 吸光度
波长(纳米): 400
信号响应: 增加
检测极限: 0.05U/mL
可重复性(%): ~ 3%
试验总时间: ~30分钟
应用实例: 谷物粉,发酵液和其他材料。
方法识别: AACC方法22-02.01,aac方法2002.01,ICC标准编号303,RACI标准方法和CCFRA(面粉测试工作组方法0018)

The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).

α-淀粉酶检测试剂盒适用于谷物和发酵液(真菌和细菌)中α-淀粉酶的特异性测定和分析。

Browse the complete list of our enzyme activity assay kits.

Scheme-K-CERA CERA Megazyme

Related products  其他相关产品:
Similar Products
K-AMYLSD – α-Amylase SD Assay Kit
K-MALTA – Malt Amylase Assay Kit
Related Carbohydrates
P-AMYL – Amylose (potato)
P-BLDX-10G – β-Limit Dextrin
P-GLYAL – Glycogen (Algae)
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
Colourimetric Substrates
R-CAAR4 – α-Amylase Reagent (Ceralpha)
R-AMHR4 – α-Amylase HR Reagent
O-BPNPC7 – Blocked 4-nitrophenyl-α-maltoheptaoside
S-RSTAR – Red Starch
I-AZAMY – AZCL-Amylose
Associated Enzymes
E-ANAAM – α-Amylase (Aspergillus oryzae)
E-BAASS – α-Amylase (Bacillus amyloliquefaciens)
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis)
E-BSTAA – α-Amylase (Thermostable) (Bacillus sp.)
E-MAST – Malt Amylase Standard

Megazyme K-TSHK 淀粉总量HK检测试剂盒

Megazyme K-TSHK 淀粉总量HK检测试剂盒

淀粉总量HK检测试剂盒
英文名: Total Starch HK Assay Kit
货号:K-TSHK
品牌: Megazyme

A modification of AOAC Method 996.11 AACC Method 76-13.01 RACI Standard Method for the measurement and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

UV-method for the determination of Total Starch in grains, 
animal feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C + DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(hexokinase)
(3) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size: 100 assays
Method: Spectrophotometric at 340 nm
Total assay time: ~ 90 min
Detection limit: 1-100% of sample weight
Application examples:
Cereal flours, food products and other materials
Method recognition:
AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard
Method No. 168), and RACI (Standard Method)

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included

AOAC 方法 996.11 AACC 方法 76-13.01 RACI 标准方法的修改,用于测量和分析谷物粉和食品中的总淀粉。该试剂盒包含改进的 α-淀粉酶,允许在 pH 5.0(以及 pH 7.0)下进行淀粉酶孵育。通过将 D-葡萄糖测定调整为基于己糖激酶/葡萄糖-6-磷酸脱氢酶/NADP+ 的格式,对该方法进行了进一步修改。

测定谷物中总淀粉的紫外法,
动物饲料、食品和其他材料

原则:
(α-淀粉酶,100°C + DMSO)
(1)淀粉颗粒+H2O→麦芽糊精

(淀粉葡萄糖苷酶)
(2)麦芽糊精+H2O→D-葡萄糖

(己糖激酶)
(3) D-葡萄糖+ATP→G-6-P+ADP

(葡萄糖-6-磷酸脱氢酶)
(4) G-6-P + NADP+ → gluconate-6-磷酸 + NADPH + H+

试剂盒大小:100 次检测
方法:340 nm 分光光度法
总检测时间:~ 90 分钟
检测限:样品重量的1-100%
应用实例:
谷物粉、食品和其他材料
方法识别:
AOAC(方法 996.11)、AACC(方法 76-13.01)、ICC(标准)
方法编号 168)和 RACI(标准方法)

好处

极具竞争力的价格(每次测试的成本)
所有试剂在制备后可稳定 > 2 年
简单格式
Mega-Calc™ 软件工具可从我们的网站获得,用于轻松处理原始数据
包含标准

Content: 100 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Total Starch
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 4 to 80 µg of D-glucose per assay
Limit of Detection: 1 g/100 g
Total Assay Time: ~ 90 min
Application examples: Cereal flours, food products and other materials.

The Total Starch HK (Hexokinase) Assay Kit for total starch determination in cereal flours and food products.

This assay kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

See our full range of dietary and starch assay kits.

Scheme-K-TSHK TSHK Megayzme

Related products
Similar Products
K-TSTA-100A – Total Starch Assay Kit (AA/AMG)
K-TSCK – Total Starch Control Flours
K-RAPRS – Resistant Starch Assay Kit (Rapid)
K-RSTAR – Resistant Starch Assay Kit
K-RSTCL – Resistant Starch Control Flours
K-SDAM – Starch Damage Assay Kit
Related Carbohydrates
P-AMYL – Amylose (potato)
P-BLDX-10G – β-Limit Dextrin
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
Colourimetric Substrates
K-CERA – α-Amylase Assay Kit (Ceralpha Method)
T-AMZ-200T – Amylazyme Tablets
S-RSTAR – Red Starch
I-AZAMY – AZCL-Amylose
Associated Enzymes
E-BSTAA – α-Amylase (Thermostable) (Bacillus sp.)
E-BLAAM-40ML – α-Amylase (Bacillus licheniformis)
E-BARBL-50KU – β-Amylase (Barley)
E-PULKP – Pullulanase M1 (Klebsiella planticola)

Megazyme K-YBGL Beta 葡聚糖[酵母和蘑菇]检测试剂盒说明书

Megazyme K-YBGL Beta 葡聚糖[酵母和蘑菇]检测试剂盒说明书

Beta葡聚糖[酵母和蘑菇]检测试剂盒 K-YBGL

β-Glucan Assay Kit (Yeast & Mushroom)

产品名称:Beta葡聚糖[酵母和蘑菇]检测试剂盒

英文名称:Mushroom and yeast beta-glucan assay Kit

货号: K-YBGL

型号规格:100次

优点:成本低;所有试剂配制后的稳定性〉2年;只有酶试剂盒可用;;方法简单;包含标准品

检测原理:

检测方法:比色法

检测波长:510nm;

总检测时间:100min;

检测限:样品重量的1-100%;

适用样品:酵母制备、蘑菇制备及其他原料;

方法认证:新方法

商品说明:

For the measurement of 1,3:1,6-ß-glucan and α-glucan in yeast preparations. Content: 100 assays per kit

Colourimetric method for the determination of Yeast and 
Mushroom β-Glucan in yeast, mushroom, foodstuffs and 
other materials

Principle:
(conc. HCl, 30°C, 45 min)
(1) 1,3:1,6-β-Glucan + 1,3-β-glucan + α-glucan + H2O → 
soluble glucan

(1.3 M HCl, 100°C, 2 h)
(2) Soluble glucan + H2O → D-glucose + laminarisaccharides (trace)

(exo-1,3-β-glucanase + β-glucosidase)
(3) Laminarisaccharides + H2O → D-glucose

(glucose oxidase)
(4) D-Glucose + H2O + O2 → D-gluconate + H2O2

(peroxidase)
(5) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine → 
 quinoneimine + 4H2O

(amyloglucosidase)
(6) α-Glucan + H2O    →    D-glucose

Kit size:                            100 assays
Method:                            Spectrophotometric at 510 nm
Total assay time:              ~ 100 min
Detection limit:                 1-100% of sample weight
Application examples:
Yeast preparations, mushroom preparations and other materials
Method recognition:        Novel method

KITS:
Kits suitable for performing 100 assays are available from Megazyme.
The kits contain the full assay method plus:
Bottle 1: (x2) exo-1,3-β-Glucanase (100 U/mL) plus
β-Glucosidase (20 U/mL) ammonium sulphate
suspension, 2.0 mL.
Stable for > 4 years at 4°C.
Bottle 2: Amyloglucosidase (1,630 U/mL) plus invertase
(500 U/mL) solution in 50% (v/v) glycerol, 20 mL.
Stable for ~ 2 years at 4°C or > 4 years below -10°C.
Bottle 3: GOPOD Reagent Buffer.
Buffer (50 mL, pH 7.4).
p-hydroxybenzoic acid and sodium azide (0.09%).
Stable for > 4 years at 4°C.
Bottle 4: GOPOD Reagent Enzymes. Glucose oxidase
plus peroxidase and 4-aminoantipyrine. Freeze-dried
powder. Stable for > 5 years below -10°C.
2
Bottle 5: D-Glucose standard solution (5 mL, 1.00 mg/mL)
in 0.2% (w/v) benzoic acid.
Stable for > 5 years; store sealed at room temperature.
Bottle 6: Control yeast β-glucan preparation (~ 2 g,
β-glucan content stated on the bottle label).
Stable for > 5 years; store sealed at room temperature

试剂盒组成:
Megazyme 提供适合进行 100 次检测的试剂盒。
该试剂盒包含完整的检测方法以及:
瓶 1:(x2)exo-1,3-β-葡聚糖酶(100 U/mL)加上
β-葡萄糖苷酶 (20 U/mL) 硫酸铵
悬浮液,2.0 毫升。
在 4°C 下可稳定 > 4 年。
瓶 2:淀粉葡萄糖苷酶 (1,630 U/mL) 加转化酶
(500 U/mL) 50% (v/v) 甘油溶液,20 mL。
在 4°C 下可稳定约 2 年或在 -10°C 以下可稳定超过 4 年。
瓶 3:GOPOD 试剂缓冲液。
缓冲液(50 毫升,pH 7.4)。
对羟基苯甲酸和叠氮化钠 (0.09%)。
在 4°C 下可稳定 > 4 年。
瓶 4:GOPOD 试剂酶。葡萄糖氧化酶
加上过氧化物酶和4-氨基安替比林。冻干
粉末。 -10°C 以下可稳定保存 > 5 年。
2
第 5 瓶:D-葡萄糖标准溶液(5 mL,1.00 mg/mL)
在 0.2% (w/v) 苯甲酸中。
稳定 > 5 年;室温密封保存。
第 6 瓶:对照酵母 β-葡聚糖制剂(~ 2 g,
瓶标签上注明的β-葡聚糖含量)。
稳定 > 5 年;室温密封保存

Advantages

  • Very cost effective
  • All reagents stable for > 12 months after preparation
  • Only enzymatic kit available
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
Content: 100 assays per kit
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: β-Glucan
Assay Format: Spectrophotometer
Detection Method: Absorbance
Wavelength (nm): 510
Signal Response: Increase
Linear Range: 4 to 100 μg of glucose per assay
Limit of Detection: 1 g/100 g
Total Assay Time: ~ 100 min
Application examples: Yeast preparations, mushroom preparations and other materials.
Method recognition: Novel method

β-Glucan (Yeast & Mushroom) Assay Kit is suitable for the measurement and analysis of 1,3:1,6-β-glucan and α-glucan in yeast and mushroom  preparations.

New, improved procedure.

Several acid and enzyme-based methods for the hydrolysis of β-glucan were compared and the best option was the method using H2SO4. For most samples, similar β-glucan values were obtained with both the optimized HCl and H2SO4 procedures. However, in the case of certain samples, specifically Ganoderma lucidum and Poria cocus, the H2SO4 procedure resulted in significantly higher values.

McCleary, B.V. & Draga, A. (2016). Measurement of β-Glucan in Mushrooms and Mycelial Products. Journal of AOAC International, Vol. 99, No. 2.

The kit components have not been altered from previous lots.

See our complete list of available polysaccharide assay kits.

scheme-K-YBGL YBGL Megazyme

Related products
Similar Products
K-EBHLG – Enzymatic Yeast β-Glucan Assay Kit
K-BGLU – β-Glucan Assay Kit (Mixed Linkage)
Related Carbohydrates
P-BGYST – β-Glucan (Yeast; Alkali Soluble)
P-PACHY – Pachyman (1,3-β-D-Glucan)
O-LAM2 – Laminaribiose
O-LAM3 – Laminaritriose
O-LAM4 – Laminaritetraose
O-LAM5 – Laminaripentaose
O-LAM6 – Laminarihexaose
O-LAM7 – Laminariheptaose
O-LAM8 – Laminarioctaose
O-LAM9 – Laminarinonaose
O-GENT – Gentiobiose
Associated Enzymes
E-LAMSE – endo-1,3-β-D-Glucanase (Trichoderma sp.)
E-LAMHV – endo-1,3-β-D-Glucanase (barley)
E-EXBGOS – exo-1,3-β-D-Glucanase + β-Glucosidase
E-EXBGTV – exo-1,3-β-D-Glucanase (Trichoderma virens)
E-BGOSTM – β-Glucosidase (Thermotoga maritima)
E-BGOSAG – β-Glucosidase (Agrobacterium sp.)
E-BGOSPC – β-Glucosidase (Phanerochaete chrysosporium)
E-BGLUC – β-Glucosidase (Aspergillus niger)
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
Related Products
D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer)

Megazyme K-GCROL 甘油检测试剂盒中文说明书

Megazyme K-GCROL 甘油检测试剂盒 Glycerol GK Assay Kit

甘油检测试剂盒 Glycerol GK Assay Kit

产品货号:K-GCROL

产品品名: 甘油检测试剂盒

英文品名 :Glycerol Assay Kit

规格型号: 70 assays per kit

甘油是食品加工业中通常使用的甜味剂和保湿剂,大多出现在运动食品和代乳品中。

上海金畔生物提供 甘油检测试剂盒 ,紫外吸光度法( 340nm )测定食品、饮料及其他原料中的甘油。

原理:

反应时间: 5min

检测限: 0.37mg/L

适用样品: 葡萄酒(葡萄汁)、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸(硬纸板)、化妆品

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.

UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials

Principle:
(glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP

(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate

(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+

Kit size: 70 assays (manual) / 700 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.34 mg/L
Application examples:
Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices,
soft drinks, toothpaste, honey, tobacco, paper (and cardboard),
cosmetics, pharmaceuticals, soap and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by OIV and
MEBAK

Content: 70 assays (manual) / 700 assays (microplate)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Glycerol
Assay Format: Spectrophotometer, Microplate
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Decrease
Linear Range: 0.8 to 35 µg of glycerol per assay
Limit of Detection: 0.34 mg/L
Reaction Time (min): ~ 5 min
Application examples: Wine (and grape juice), beer, spirits, vinegar, marzipan, fruit juices, soft drinks, toothpaste, honey, tobacco, paper (and cardboard), cosmetics, pharmaceuticals, soap and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Methods based on this principle have been accepted by OIV and MEBAK

The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuantTM  Wave Spectrophotometer (D-MQWAVE).

Browse our wide range of alcohol assay kit products.

Scheme-K-GCROL GCROL Megazyme

Advantages
  • Novel format for increased stability
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual and microplate formats
  • Extended cofactors stability
内容: 70次化验(手册)/700次试验(微版)
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 甘油
化验格式: 分光光度计,微版
检测方法: 吸光度
波长(纳米): 340
信号响应: 减少
线性范围: 每次测定甘油0.8至35微克
检测极限: 0.34毫克/升
反应时间(最低): ~5分钟
应用实例: 葡萄酒(和葡萄汁)、啤酒、烈酒、醋、杏仁锅、果汁、软饮料、牙膏、蜂蜜、烟草、纸张(和纸板)、化妆品、药品、肥皂和其他材料(例如生物培养物、样品等)。
方法识别: 基于这一原则的方法已被OIV和MEBAK所接受。

甘油检测试剂盒是一种简单、可靠、快速、准确的测定和分析饮料、食品和其他材料中甘油的方法。

内容说明:如果将所有卷减少一半,每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).

浏览我们范围广泛的酒精分析试剂盒产品。

Scheme-K-GCROL GCROL Megazyme

优势
  • 增加稳定性的新格式
  • 极具竞争力的价格(每次测试成本)
  • 所有试剂按供应情况稳定超过2年
  • 极快速反应
  • 特尔加-卡尔克™软件工具可从我们的网站获得无麻烦的原始数据处理。
  • 标准包括
  • 适用于手动和微型制版