Megazyme K-AVCHO 可用的碳水化合物检测试剂盒
可用的碳水化合物检测试剂盒
英文名：Available Carbohydrates Assay Kit
货号：K-AVCHO
规格：100 Assays of each per kit
品牌： Megazyme
| Content: | 100 assays of each per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Available Carbohydrates, Dietary Fiber |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 μg of D-glucose, D-fructose or D-galactose per assay |
| Limit of Detection: | 1.475 g/100 g |
| Reaction Time (min): | ~ 5 h |
| Application examples: | Food ingredients, food products and other materials. |
The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving ‘First Action’ status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).
* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.
| 内容： | 每个试剂盒100次化验 |
| 船运温度： | 环境 |
| 储存温度： | 短期稳定性：2-8oC， 长期稳定性：见单个组件标签 |
| 稳定性： | >2年在建议的储存条件下 |
| 分析物： | 有效碳水化合物，膳食纤维 |
| 化验格式： | 分光光度计 |
| 检测方法： | 吸光度 |
| 波长(纳米)： | 340 |
| 信号响应： | 增加 |
| 线性范围： | 4~80μg D-葡萄糖、D-果糖或D-半乳糖 |
| 检测极限： | 1.475克/100克 |
| 反应时间(最低)： | ~5h |
| 应用实例： | 食品配料、食品及其他材料。 |
这个可用碳水化合物分析试剂盒方法适用于总可消化淀粉(TDS)加麦芽糊精、蔗糖、D-葡萄糖、D-果糖和乳糖的有效碳水化合物(AVCHO)的测定。一种新的接收“第一动作”状态的改进方法：aac 2020.07。该方法是为模拟而设计的。体内人体小肠条件(即与PAA+AMG孵育4h)与膳食纤维(DF)方法学的最新进展(K-RINTDF：AOAC方法2017.16)，并根据2009年食品法典宣布的新的(基于生理的)DF定义。此外，蔗糖被一种特殊的“蔗糖酶”水解，而蔗糖酶(不同于传统上用于此反应的转化酶)对低聚果糖(Fos)没有作用。
*总可消化淀粉(TDS)是指在4小时内消化的淀粉，是人体小肠中可供消化和吸收的碳水化合物的一部分。
Related products
Similar Products
K-ACHDF – Available Carbohydrates/Dietary Fiber Assay Kit
K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit
K-DSTRS – Digestible and Resistant Starch Assay Kit
K-RAPRS – Resistant Starch Assay Kit (Rapid)
K-TDFC – Total Dietary Fiber Controls
Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
E-BGLAN – β-Galactosidase (Aspergillus niger)
E-SUCR – Sucrase (from yeast)