Megazyme K-ETOH 乙醇检测试剂盒说明书

发表于2021年10月13日由上海金畔生物科技有限公司

Megazyme K-ETOH 乙醇检测试剂盒说明书
Megazyme试剂盒
Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。
Megazyme K-ETOH 乙醇检测试剂盒
中文名称：乙醇检测试剂盒
英文名：Ethanol Assay Kit
货号：K-ETOH
规格：60 assays (manual)

The Ethanol test kit is a simple, reliable and accurate method for the measurement and analysis of ethanol in beverages and foodstuffs.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Ethanol in foodstuffs, beverages, and other materials

Principle原理:
(alcohol dehydrogenase)
(1) Ethanol + NAD+ ↔ acetaldehyde + NADH + H+

(aldehyde dehydrogenase)
(2) Acetaldehyde + NAD+ + H2O → acetic acid + NADH + H+

Kit size: 60 assays (manual) / 600 (microplate)
/ 600 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 5 min
Detection limit: 0.093 mg/L
Application examples:
Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic
/ non-alcoholic beverages, pickles, fruit and fruit juice, chocolate
products, vinegar, jam, bread and bakery products, honey, soy sauce,
dairy products, cosmetics, pharmaceuticals and other materials
(e.g. biological cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by IFU, EBC,
MEBAK and ASBC

Advantages 优点：

Simple format – aldehyde dehydrogenase supplied as stable suspension

Very competitive price (cost per test)

All reagents stable for > 2 years after preparation

Rapid reaction

Mega-Calc™ software tool is available from our website for hassle-free raw data processing

Standard included

Extended cofactors stability

Suitable for manual, microplate and auto-analyser formats

Content:	60 assays (manual) / 600 assays (microplate) / 600 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Ethanol
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	0.25 to 12 µg of ethanol per assay
Limit of Detection:	0.093 mg/L
Reaction Time (min):	~ 5 min
Application examples:	Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic / non-alcoholic beverages, pickles, fruit and fruit juice, chocolate products, vinegar, jam, bread and bakery products, honey, soy sauce, dairy products, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by AOAC (AOAC Method 2019.08, First Action), IFU, EBC, MEBAK and ASBC

The Ethanol test kit is a simple, reliable and accurate method for the measurement and analysis of ethanol in beverages and foodstuffs.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuant^TM Wave Spectrophotometer (D-MQWAVE).

内容：	60项试验(手册)/600项试验(微板)/600项试验(自动分析仪)
船运温度：	环境
储存温度：	短期稳定性：2-8^oC，长期稳定性：见单个组件标签
稳定性：	>2年在建议的储存条件下

分析物：	乙醇
化验格式：	分光光度计，微板，自动分析仪
检测方法：	吸光度
波长(纳米)：	340
信号响应：	增加
线性范围：	每次测定乙醇0.25至12微克
检测极限：	0.093毫克/升
反应时间(最低)：	~5分钟
应用实例：	葡萄酒、啤酒、苹果酒、含酒精果汁、烈酒、利口酒、低酒精/不含酒精饮料、泡菜、水果和果汁、巧克力产品、醋、果酱、面包和面包店产品、蜂蜜、酱油、奶制品、化妆品、药品和其他材料(如生物培养物、样品等)。
方法识别：	基于这一原则的方法已被aaac(aac方法2019.08，First Action)、ifu、ebc、mebak和asbc所接受。

乙醇检测试剂盒是测定和分析饮料和食品中乙醇的一种简便、可靠和准确的方法。

内容说明：如果将所有卷减少一半，每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。^TM波分光光度计D-MQWAVE).

Scheme-K-ETOH ETOH megayzme

Related Products
K-ETOHLQR – Ethanol Assay Kit (Liquid Ready) Assay Kit
K-LMAL-116A – L-Malic Acid Assay Kit (Manual Format)
K-ACETRM – Acetic Acid Assay Kit (Acetate Kinase Manual Format)
K-FRUGL – D-Fructose/D-Glucose Assay Kit
K-LARGE – L-Arginine/Urea/Ammonia Assay Kit
Related Equipment
D-CHEM2910 – ChemWell® 2910 Automated EIA and Chemistry Analyzer
D-CHEMT – ChemWell®-T Automated Chemistry Analyzer
D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer)

Megazyme K-FRHYD 甲醛检测试剂盒

发表于2021年5月1日由上海金畔生物科技有限公司

Megazyme K-FRHYD 甲醛检测试剂盒
英文名：Formaldehyde Assay Kit
货号：K-FRHYD
规格：50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
品牌： Megazyme

The Histamine Assay Kit provides a simple robust method for the measurement of histamine.

Content:	50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Formaldehyde
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	0.1 to 14 μg of formaldehyde per assay
Limit of Detection:	0.033 mg of formaldehyde per test or 0.016 mg/mL of formaldehyde in a sample treated as per the standard procedure
Limit of Quantification:	0.11 mg of formaldehyde per test or 0.054 mg/mL of formaldehyde in a sample treated as per the standard procedure
Reproducibility (%):	~ 3%
Reaction Time (min):	~ 15 min
Application examples:	Environmental samples, food and beverage samples.

组胺分析试剂盒为组胺的测定提供了一种简单、可靠的方法。

内容：	50项试验(手册)/500项试验(微板)/500项试验(自动分析仪)
船运温度：	环境
储存温度：	短期稳定性：2-8oC，长期稳定性：见单个组件标签
稳定性：	>2年在建议的储存条件下

分析物：	甲醛
化验格式：	分光光度计，微板，自动分析仪
检测方法：	吸光度
波长(纳米)：	340
信号响应：	增加
线性范围：	每次测定甲醛0.1~14μg
检测极限：	每次试验甲醛0.033毫克或按标准程序处理的样品中甲醛含量为0.016毫克/毫升
数量限制：	每次试验甲醛0.11毫克或按标准程序处理的样品中甲醛含量为0.054毫克/毫升
可重复性(%)：	~ 3%
反应时间(最低)：	~15分钟
应用实例：	环境样品，食品和饮料样品。

Megazyme K-ETOHLQR 乙醇分析试剂盒（液态）说明书

发表于2021年5月1日由上海金畔生物科技有限公司

Megazyme K-ETOHLQR 乙醇分析试剂盒（液态）说明书
英文名：Ethanol Assay Kit (Liquid Ready) Assay Kit
货号：K-ETOHLQR
规格：60 assays (manual)
品牌： Megazyme

The Ethanol (Liquid Ready) assay kit is a rapid, simple, reliable and accurate method for the specific measurement and analysis of ethanol in wine, beverages, foodstuffs and other materials. Supplied as a “ready to use” liquid stable formulation that is suitable for manual auto-analyser and microplate formats.

Suitable for manual, auto-analyser and microplate formats.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).

See more of our assay kits for alcohols.

Content: 60 assays (manual) / 600 assays (microplate) / 600 assays (auto-analyser)

Shipping Temperature: Ambient

Storage Temperature: Short term stability: 2-8oC,

Long term stability: See individual component labels

Stability: > 2 years under recommended storage conditions

Analyte: Ethanol

Assay Format: Spectrophotometer, Microplate, Auto-analyser

Detection Method: Absorbance

Wavelength (nm): 340

Signal Response: Increase

Linear Range: 0.25 to 30 µg of ethanol per assay

Limit of Detection: 1.6 mg/L

Reaction Time (min): ~ 7 min

Application examples: Wine, beer, cider, alcoholic fruit juices, spirits, liqueurs, low-alcoholic / non-alcoholic beverages, pickles, fruit and fruit juice, chocolate products, vinegar, jam, bread and bakery products, honey, soy sauce, dairy products, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).

Method recognition: Methods based on this principle have been accepted by AOAC (AOAC© Official MethodSM 2017.07)

Megazyme K-AVCHO 可用的碳水化合物检测试剂盒

发表于2021年5月1日由上海金畔生物科技有限公司

Megazyme K-AVCHO 可用的碳水化合物检测试剂盒

可用的碳水化合物检测试剂盒
英文名：Available Carbohydrates Assay Kit
货号：K-AVCHO
规格：100 Assays of each per kit
品牌： Megazyme

Content:	100 assays of each per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Available Carbohydrates, Dietary Fiber
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 μg of D-glucose, D-fructose or D-galactose per assay
Limit of Detection:	1.475 g/100 g
Reaction Time (min):	~ 5 h
Application examples:	Food ingredients, food products and other materials.

The Available Carbohydrates Assay Kit method is suitable for the determination of available carbohydrates (AVCHO) comprising *total digestible starch (TDS) plus maltodextrins, sucrose, D-glucose, D-fructose and lactose. New Improved method receiving ‘First Action’ status: AOAC 2020.07. This method is designed to simulate in vivo conditions in the human small intestine (i.e. a 4 h incubation time with PAA + AMG) in parallel with recent advances in Dietary Fiber (DF) methodology (K-RINTDF: AOAC Method 2017.16) and in accordance with the new (physiological based) definition of DF announced by Codex Alimentarius in 2009. Also, sucrose is hydrolysed with a specific “sucrase” enzyme which (unlike invertase which has been used traditionally for this reaction) has no action on fructo-oligosaccharides (FOS).

* Total digestible starch (TDS) is defined as starch that is digested in a 4 h period and is part of the carbohydrate that is available for digestion and absorption in the human small intestine.

Scheme-K-AVCHO AVCHO Megazyme

内容：	每个试剂盒100次化验
船运温度：	环境
储存温度：	短期稳定性：2-8^oC，长期稳定性：见单个组件标签
稳定性：	>2年在建议的储存条件下

分析物：	有效碳水化合物，膳食纤维
化验格式：	分光光度计
检测方法：	吸光度
波长(纳米)：	340
信号响应：	增加
线性范围：	4~80μg D-葡萄糖、D-果糖或D-半乳糖
检测极限：	1.475克/100克
反应时间(最低)：	~5h
应用实例：	食品配料、食品及其他材料。

这个可用碳水化合物分析试剂盒方法适用于总可消化淀粉(TDS)加麦芽糊精、蔗糖、D-葡萄糖、D-果糖和乳糖的有效碳水化合物(AVCHO)的测定。一种新的接收“第一动作”状态的改进方法：aac 2020.07。该方法是为模拟而设计的。体内人体小肠条件(即与PAA+AMG孵育4h)与膳食纤维(DF)方法学的最新进展(K-RINTDF：AOAC方法2017.16)，并根据2009年食品法典宣布的新的(基于生理的)DF定义。此外，蔗糖被一种特殊的“蔗糖酶”水解，而蔗糖酶(不同于传统上用于此反应的转化酶)对低聚果糖(Fos)没有作用。

*总可消化淀粉(TDS)是指在4小时内消化的淀粉，是人体小肠中可供消化和吸收的碳水化合物的一部分。

Related products
Similar Products
K-ACHDF – Available Carbohydrates/Dietary Fiber Assay Kit
K-RINTDF – Rapid Integrated Total Dietary Fiber Assay Kit
K-DSTRS – Digestible and Resistant Starch Assay Kit
K-RAPRS – Resistant Starch Assay Kit (Rapid)
K-TDFC – Total Dietary Fiber Controls
Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
E-BGLAN – β-Galactosidase (Aspergillus niger)
E-SUCR – Sucrase (from yeast)

Megazyme R-AMGR3 淀粉葡萄糖苷酶检测试剂中文说明书

发表于2021年4月26日由上海金畔生物科技有限公司

Megazyme R-AMGR3 淀粉葡萄糖苷酶检测试剂中文说明书

淀粉葡萄糖苷酶测定试剂
英文名：Amyloglucosidase Assay Reagent
货号：R-AMGR3
规格：200次检测Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息。

Content:	200 assays per kit (4 vials)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Amyloglucosidase
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	400
Limit of Detection:	~ 2.5 U/mL
Reproducibility (%):	~ 3%
Reaction Time (min):	~ 15 min

内容：	每盒200次化验(4小瓶)
船运温度：	环境
储存温度：	短期稳定性：2-8^oC，长期稳定性：见单个组件标签
稳定性：	>2年在建议的储存条件下

分析物：	氨基葡萄糖苷酶
化验格式：	分光光度计
检测方法：	吸光度
波长(纳米)：	400
检测极限：	~2.5U/mL
可重复性(%)：	~ 3%
反应时间(最低)：	~15分钟

高纯度淀粉葡萄糖苷酶分析试剂-4瓶，用于淀粉糖苷酶的测定，用于研究，生化酶分析和离体诊断分析

p-硝基苯β-D-麦芽苷加过量的β-葡萄糖苷酶.

Related Carbohydrates
P-AMYL – Amylose (potato)
O-PAN-1G – Panose
O-GMT – 63-α-D-Glucosyl-maltotriose
O-GMH – 63-α-D-Glucosyl-maltotriosyl-maltotriose
O-MTMT – 63-α-D-Maltotriosyl-maltotriose
O-MAL3 – Maltotriose
O-MAL4 – Maltotetraose
O-MAL5 – Maltopentaose
O-MAL6 – Maltohexaose
O-MAL7 – Maltoheptaose
O-MAL8 – Maltooctaose
O-IPAN – Isopanose
Associated Enzymes
E-AMGDF-40ML – Amyloglucosidase (Aspergillus niger)
E-AMGDFNG-20ML – Amyloglucosidase (Aspergillus niger)
Glycerol Free
E-AMGDFPD – Amyloglucosidase (Aspergillus niger) Powder
E-AMGFR-100MG – Amyloglucosidase (Aspergillus niger)
E-AMGPU – Amyloglucosidase (Rhizopus sp.)

上海金畔生物代理Megazyme品牌试剂产品，欢迎新老客户访问Megazyme官网或者咨询我们获取更多相关产品信息。
图片仅供参考,请以实物为准。
正文中列出的所有试剂只能用于测试或研究，不能作为”药品”，”食品”，”家庭用品”等使用。
我司所销售的化学试剂、原料等所有产品（包括但不限于抗生素类、蛋白质类、试剂盒类产品等）仅限用于科学研究用途，不得作用于人体。

Megazyme K-XylX6-2V 内切木聚糖酶测定试剂盒（xylx6法）

发表于2021年4月13日由上海金畔生物科技有限公司

Megazyme K-XylX6-2V 内切木聚糖酶测定试剂盒（xylx6法）

Megazyme试剂盒代理、Megazyme代理
欢迎访问Megazyme官网或者咨询我们获取更多产品信息
内切木聚糖酶测定试剂盒（xylx6法）
英文名：endo-Xylanase Assay Kit (XylX6 Method)
货号：K-XYLX6-1V
规格：100 assays (manual) / 200 assays (auto-analyser)
品牌： Megazyme

Megazyme K-XYLX6 内切木聚糖酶测定试剂盒（xylx6法）

The XylX6 assay reagent for the measurement of endo-xylanase (endo-1,4-β-xylanase) contains two components; 1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-4⁵-glucosyl-xylopentaoside and 2) β-xylosidase. The ketone blocking group prevents any hydrolytic action by the β-xylosidase or other exo-acting glycosidases on the XylX6 substrate. Incubation with an endo-xylanase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-xylosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of XylX6 by the endo-xylanase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Colourimetric method for the determination of endo-1,4-β-xylanase in any
sample type including crude enzyme extracts, fermentation broths or
commercial enzyme preparations

Principle:
(endo-1,4-β-xylanase)
(1) 3-Ketobutylidene-GX₅-β-PNP + H₂O → Blocked-GX_Y + X_(5-Y)-β-PNP

(β-glucosidase)
(2) X_(5-Y)-β-PNP + H₂O → D-xylose + PNP

(alkaline solution)
(3) PNP → phenolate ion (yellow colour)
Note: PNP = 4-nitrophenol

Kit size:
K-XylX6-1V 100 assays (manual) / 200 (auto-analyser)
or
K-XylX6-2V 200 assays (manual) / 400 (auto-analyser)

Method:                      Spectrophotometric at 400/405 nm
Total assay time:        10 min
Detection limit:                5.3 x 10^-4 U/mL
Application examples:
Fermentation broths, industrial enzyme preparations, animal feed, biofuels research, barley
malt analysis
Method recognition:     Novel method

用于测量内木聚糖酶（内-1,4-β-木聚糖酶）的XylX6分析试剂包含两个成分; 1）4,6-O-（3-酮丁烯）-4-硝基苯基-β-D-45-葡糖基-木糖基opentaoside和2）β-木糖苷酶。酮保护基团可防止XylX6底物上的β-木糖苷酶或其他exo-acting糖苷酶产生任何水解作用。与木聚糖内切酶一起温育会产生非封闭的比色寡糖，该寡糖会被辅助β-木糖苷酶快速水解。因此，4-硝基苯酚的生成速率与内木聚糖酶水解XylX6直接相关。加入Tris缓冲溶液（pH = 10.0）后，反应终止，苯酚盐显色。

用比色法测定任何动物体内的1,4-β-木聚糖内切酶
样品类型，包括粗酶提取物，发酵液或
商业酶制剂

原则：
（1,4-β-木聚糖内切酶）
（1）3-酮丁烯-GX5-β-PNP+ H2O→嵌段-GXY + X（5-Y）-β-PNP

（β-葡萄糖苷酶）
（2）X（5-Y）-β-PNP+ H2O→D-木糖+ PNP

（碱性溶液）
（3）PNP→酚盐离子（黄色）
注意：PNP = 4-硝基苯酚

套件尺寸：
K-XylX6-1V 100次检测（手动）/ 200次（自动分析器）
或者
K-XylX6-2V 200次检测（手动）/ 400次（自动分析器）

方法：分光光度法在400/405 nm下进行分光光度法
总测定时间：10分钟
检测限：5.3 x 10-4 U / mL
应用实例：
发酵液，工业酶制剂，动物饲料，生物燃料研究，大麦
麦芽分析
方法识别：新颖方法

Megazyme K-LARGE L-精氨酸/尿素/氨检测试剂盒[快速] 中文说明书

发表于2021年4月12日由上海金畔生物科技有限公司

Megazyme K-LARGE L-精氨酸/尿素/氨检测试剂盒[快速] 中文说明书

L-Arginine/Urea/Ammonia (Rapid)

L-精氨酸/尿素/氨检测试剂盒[快速]
英文名：L-Arginine/Urea/Ammonia (Rapid)
货号：K-LARGE
规格：150 assays – 50 of each component

精氨酸是维持婴幼儿生长发育必不可少的氨基酸。它是鸟氨酸循环的中间代谢物，能促使氨转变成为尿素，从而降低血氨含量。它也是精子蛋白的主要成分，有促进精子生成，提供精子运动能量的作用。此外，静注精氨酸，能刺激垂体释放生长激素，可用于垂体功能试验。精氨酸可用作营养增补剂；与糖进行加热反应（氨基-羰基反应）可获得特殊的香味物质。GB 2760-2001规定为允许使用的食品用香料。氨基酸输液及综合氨基酸制剂的重要成分。

上海金畔生物提供L-精氨酸/尿素/氨[快速]检测试剂盒，采用紫外分光光度计法（340nm）测定葡萄汁、酒中的L-精氨酸，尿素和氢。

商品说明：

For the specific and rapid assay of L-arginine, urea and ammonia in grape juice/must and wine. Content:150 assays – 50 of each component

UV-method for the determination of L-Arginine, Urea and Ammonia
in grape juice, must and wine

Principle:
(microbial glutamate dehydrogenase)
(1) 2-Oxoglutarate + NADPH + NH₄⁺ → L-glutamic acid + NADP⁺ +
H₂O
(urease)
(2) Urea + H₂O → 2 NH₃ + CO₂

(arginase)
(3) L-Arginine + H₂O → urea + ornithine

Kit size:                            50 assays
Method:                              Spectrophotometric at 340 nm
Reaction time:                    ~ 20 min [ammonia (2 min), urea (6 min),
L-arginine (7 min)] Detection limit:                  0.07 mg/L (ammonia)
0.13 mg/L (urea)
0.37 mg/L (L-arginine)
Application examples:
Grape juice, wine must, wine and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:        Improved method

Advantages

Improved assay format
Very rapid reactions due to use of uninhibited glutamate dehydrogenase
All enzymes supplied as stabilised suspensions
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Extended cofactors stability

内容：	每个试剂盒50次
船运温度：	环境
储存温度：	短期稳定性：2-8^oC，长期稳定性：见单个组件标签
稳定性：	>2年在建议的储存条件下

分析物：	氨，L-精氨酸，氮，尿素，燕
化验格式：	分光光度计
检测方法：	吸光度
波长(纳米)：	340
信号响应：	减少
线性范围：	每组测定L-精氨酸1.0~35 mg，氨0.2~7.0μg，尿素0.3~14 g。
检测极限：	0.07毫克/升(氨)， 0.13毫克/升(尿素)， 0.37毫克/升(L-精氨酸)
反应时间(最低)：	~20 min[氨(2 Min)、尿素(6 Min)、L-精氨酸(7 Min)]
应用实例：	葡萄汁、葡萄酒、葡萄酒和其他材料(如生物培养、样品等)。
方法识别：	改进方法

L-精氨酸/尿素/氨试剂盒专属性强，快速测定和分析葡萄汁/必须和葡萄酒中的L-精氨酸、尿素和氨。

内容说明：如果将所有卷减少一半，每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。^TM波分光光度计D-MQWAVE).

Scheme-K-LARGE LARGE Megazyme

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K-GLNAM – L-Glutamine/Ammonia Assay Kit (Rapid)
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索取产品说明书请联系：18301939375 QQ:3259632176

Megazyme K-TREH 海藻糖检测试剂盒中文说明书

发表于2021年3月16日由上海金畔生物科技有限公司

Megazyme K-TREH 海藻糖检测试剂盒中文说明书

海藻糖检测试剂盒
英文名：Trehalose Assay Kit
货号：K-TREH
规格：Sufficient for 6 Controls
品牌： Megazyme

The Trehalose test kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Trehalose and
D-Glucose in foodstuffs, beverages, and other materials

Principle:
(trehalase)
(1) Trehalose + H₂O → D-glucose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 8 min
Detection limit: 37.5 mg/L
Application examples:
Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp),
fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits
and vegetables, fruit products, white chocolate, sports drinks, dairy
products, egg products, soups and sauces, confectionery, chewing gum,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel method

Advantages

Only enzymatic kit available
Very cost effective
All reagents stable for > 2 years after preparation
Very rapid reaction
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Suitable for manual, microplate and auto-analyser formats

海藻糖检测试剂盒是一种用于快速，可靠地测量和分析食品，饮料和其他材料中海藻糖的简便方法。

适用于手动，自动分析仪和微孔板格式。

紫外线法测定海藻糖和
食品，饮料和其他材料中的D-葡萄糖

原则：
（海藻糖）
（1）海藻糖+ H2O→D-葡萄糖

（己糖激酶）
（2）D-葡萄糖+ ATP→G-6-P + ADP

（6-磷酸葡萄糖脱氢酶）
（3）G-6-P + NADP +→葡萄糖酸6-磷酸酯+ NADPH + H +

试剂盒大小：100个测定（手动）/ 1000个（微孔板）
/ 1100（自动分析仪）
方法：在340 nm处进行分光光度法
反应时间：〜8分钟
检出限：37.5 mg / L
应用实例：
蜂蜜，蘑菇，面包，啤酒，海鲜（例如龙虾和虾），
果汁，果泥和馅料，营养棒，鱼糜，脱水水果
和蔬菜，水果产品，白巧克力，运动饮料，乳制品
产品，蛋制品，汤和酱料，糖果，口香糖，
化妆品，药品和其他材料（例如生物培养物，
样本等）
方法识别：新方法

优点：

仅提供酶试剂盒
极具成本效益
制备后所有试剂均稳定> 2年
反应非常迅速
可从我们的网站上获得Mega-Calc™软件工具，以进行无忧的原始数据处理
含标准
适用于手动，微孔板和自动分析仪格式

Content:	100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Trehalose
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 µg of trehalose per assay
Limit of Detection:	37.5 mg/L
Reaction Time (min):	~ 10 min
Application examples:	Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp), fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits and vegetables, fruit products, white chocolate, sports drinks, dairy products, egg products, soups and sauces, confectionery, chewing gum, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	*Novel method*

The Trehalose assay kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuant^TM Wave Spectrophotometer (D-MQWAVE).

内容：	100次(人工)/1000次(微板)/1100次(自动分析仪)
船运温度：	环境
储存温度：	短期稳定性：2-8^oC，长期稳定性：见单个组件标签
稳定性：	>2年在建议的储存条件下

分析物：	海藻糖
化验格式：	分光光度计，微板，自动分析仪
检测方法：	吸光度
波长(纳米)：	340
信号响应：	增加
线性范围：	每次测定含海藻糖4~80克
检测极限：	37.5毫克/升
反应时间(最低)：	~10分钟
应用实例：	蜂蜜、蘑菇、面包、啤酒、海鲜(如龙虾和虾)、果汁、果酱和填料、营养棒、鱼糜、脱水水果和蔬菜、水果制品、白巧克力、运动饮料、奶制品、鸡蛋制品、汤和酱汁、糖果、口香糖、化妆品、药品和其他材料(例如生物培养、样品等)。
方法识别：	*新方法*

海藻糖测定试剂盒是一种快速、可靠地测定和分析食品、饮料和其他物质中海藻糖的简便方法。

内容说明：如果将所有卷减少一半，每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。^TM波分光光度计D-MQWAVE).

Scheme-K-TREH TREH Megazyme

Related products
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K-GCROL – Glycerol Assay Kit
K-GLUC – D-Glucose Assay Kit (GOPOD Format)
K-GLUHK-220A – D-Glucose HK Assay Kit
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D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer)

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