Megazyme K-TREH 海藻糖检测试剂盒中文说明书

Megazyme K-TREH 海藻糖检测试剂盒中文说明书

海藻糖检测试剂盒
英文名:Trehalose Assay Kit
货号:K-TREH
规格:Sufficient for 6 Controls
品牌: Megazyme

The Trehalose test kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Suitable for manual, auto-analyser and microplate formats.

UV-method for the determination of Trehalose and 
D-Glucose in foodstuffs, beverages, and other materials

Principle:
(trehalase)
(1) Trehalose + H2O → D-glucose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

Kit size: 100 assays (manual) / 1000 (microplate)
/ 1100 (auto-analyser)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 8 min
Detection limit: 37.5 mg/L
Application examples:
Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp),
fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits
and vegetables, fruit products, white chocolate, sports drinks, dairy
products, egg products, soups and sauces, confectionery, chewing gum,
cosmetics, pharmaceuticals and other materials (e.g. biological cultures,
samples, etc.)
Method recognition: Novel method

Advantages

  • Only enzymatic kit available
  • Very cost effective
  • All reagents stable for > 2 years after preparation
  • Very rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

海藻糖检测试剂盒是一种用于快速,可靠地测量和分析食品,饮料和其他材料中海藻糖的简便方法。

适用于手动,自动分析仪和微孔板格式。

紫外线法测定海藻糖和
食品,饮料和其他材料中的D-葡萄糖

原则:
(海藻糖)
(1)海藻糖+ H2O→D-葡萄糖

(己糖激酶)
(2)D-葡萄糖+ ATP→G-6-P + ADP

(6-磷酸葡萄糖脱氢酶)
(3)G-6-P + NADP +→葡萄糖酸6-磷酸酯+ NADPH + H +

试剂盒大小:100个测定(手动)/ 1000个(微孔板)
/ 1100(自动分析仪)
方法:在340 nm处进行分光光度法
反应时间:〜8分钟
检出限:37.5 mg / L
应用实例:
蜂蜜,蘑菇,面包,啤酒,海鲜(例如龙虾和虾),
果汁,果泥和馅料,营养棒,鱼糜,脱水水果
和蔬菜,水果产品,白巧克力,运动饮料,乳制品
产品,蛋制品,汤和酱料,糖果,口香糖,
化妆品,药​​品和其他材料(例如生物培养物,
样本等)
方法识别:新方法

优点:

仅提供酶试剂盒
极具成本效益
制备后所有试剂均稳定> 2年
反应非常迅速
可从我们的网站上获得Mega-Calc™软件工具,以进行无忧的原始数据处理
含标准
适用于手动,微孔板和自动分析仪格式

Content: 100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Trehalose
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 340
Signal Response: Increase
Linear Range: 4 to 80 µg of trehalose per assay
Limit of Detection: 37.5 mg/L
Reaction Time (min): ~ 10 min
Application examples: Honey, mushrooms, bread, beer, seafood (e.g. lobster and shrimp), fruit juices, purees and fillings, nutrition bars, surimi, dehydrated fruits and vegetables, fruit products, white chocolate, sports drinks, dairy products, egg products, soups and sauces, confectionery, chewing gum, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition: Novel method

The Trehalose assay kit is a simple method for the rapid and reliable measurement and analysis of trehalose in foods, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).

内容: 100次(人工)/1000次(微板)/1100次(自动分析仪)
船运温度: 环境
储存温度: 短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性: >2年在建议的储存条件下
分析物: 海藻糖
化验格式: 分光光度计,微板,自动分析仪
检测方法: 吸光度
波长(纳米): 340
信号响应: 增加
线性范围: 每次测定含海藻糖4~80克
检测极限: 37.5毫克/升
反应时间(最低): ~10分钟
应用实例: 蜂蜜、蘑菇、面包、啤酒、海鲜(如龙虾和虾)、果汁、果酱和填料、营养棒、鱼糜、脱水水果和蔬菜、水果制品、白巧克力、运动饮料、奶制品、鸡蛋制品、汤和酱汁、糖果、口香糖、化妆品、药品和其他材料(例如生物培养、样品等)。
方法识别: 新方法

海藻糖测定试剂盒是一种快速、可靠地测定和分析食品、饮料和其他物质中海藻糖的简便方法。

内容说明:如果将所有卷减少一半,每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).

Scheme-K-TREH TREH Megazyme

Related products
Related Products
K-GCROL – Glycerol Assay Kit
K-GLUC – D-Glucose Assay Kit (GOPOD Format)
K-GLUHK-220A – D-Glucose HK Assay Kit
Related Equipment
D-MQWAVE-1 – MegaQuant™ Wave Spectrophotometer / MegaQuant™ Wave Starter Pack (Chemistry Analyzer)

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