Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)-Megazyme试剂盒

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)-Megazyme试剂盒
麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒
英文名:Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
货号:K-MBG4
规格:100 assays (manual) / 400 assays (auto-analyser)
品牌: Megazyme
Product code: K-MBG4
€257.00

100 assays (manual) / 400 assays (auto-analyser)

Prices exclude VAT

Available for shipping

Description Documents (42) Related Products Publications (2) Safety Information
Content: (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or
(Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: β-Glucanase/Lichenase
Assay Format: Spectrophotometer, Auto-analyser
Detection Method: Absorbance
Wavelength (nm): 400
Signal Response: Increase
Limit of Detection: (Malt β-glucanase) 4.3 x 10-4 U/mL Or
(Lichenase) 9.1 x 10-5 U/mL
Reproducibility (%): ~ 3%
Total Assay Time: (Malt β-glucanase) ~ 20 min Or
(Lichenase) ~ 10 min
Application examples: Crude malt extracts, industrial enzyme preparations.
Method recognition: Novel method

含量:(麦芽 β-葡聚糖酶)100 次检测(手动)/400 次(自动分析仪)或
(地衣酶)100 / 200 次检测(手动)/330 次(自动分析仪)
运输温度:环境
储存温度:短期稳定性:2-8oC,
长期稳定性:见单个组件标签
稳定性:在推荐的储存条件下 > 2 年
分析物:β-葡聚糖酶/地衣酶
检测形式:分光光度计、自动分析仪
检测方法:吸光度
波长(nm):400
信号响应:增加
检测限:(麦芽 β-葡聚糖酶)4.3 x 10-4 U/mL 或
(地衣酶) 9.1 x 10-5 U/mL
再现性(%):~ 3%
总测定时间:(麦芽 β-葡聚糖酶)~ 20 分钟或
(地衣酶)~ 10 分钟
应用实例:粗麦芽提取物、工业酶制剂。
方法识别:新方法

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

Discover more assay kits for enzyme activity measurement.

MBG4 试剂含有单一底物,即 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4)。 亚苄基乙缩醛基团可防止外作用水解酶(如 β-葡糖苷酶或纤维二糖水解酶)的任何水解作用。

混合连接 β-葡聚糖酶 (endo-1,3:1,4-β-glucanase) / 地衣酶 (EC 3.2.1.73) 专门用于从该底物释放 2-氯-4-硝基苯酚 (CNP)。 CNP 的释放速率与样品中的 β-葡聚糖酶/地衣酶活性直接相关。 反应终止,加入 Tris 缓冲溶液 (pH = 10.0) 后酚盐颜色显色。

请注意,底物不会被 β-葡萄糖苷酶或纤维二糖水解酶水解。 底物可以被某些内切纤维素酶(例如木霉属)水解,但这不会导致吸光度增加。

发现更多用于酶活性测量的检测试剂盒。

Data calculators are located in the Documents tab.

Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

Advantages
  • Very cost effective
  • All reagents stable for > 2 years
  • Specific for endo-1,3:1,4-β-glucanase/lichenase
  • Simple, convenient, rapid assay
  • Well suited to automation
  • Malt flour standard and lichenase standard included
Documents
Certificate of Analysis
Select COA based on lot# 200211 190705 160508
Safety Data Sheet
Assay Protocols
Assay Protocols K-MBG4_LICHENASE_D-CHEM2910 K-MBG4_LICHENASE_D-CHEMT K-MBG4_MALT_D-CHEM2910 K-MBG4_MALT_D-CHEMT
Multiple Mega-Calcs
Select Mega-Calc K-MBG4_LICHENASE_CALC K-MBG4_MALT_CALC

FAQs Assay Protocol Data Calculator

Related products
Similar Products
K-MBGL – β-Glucanase Assay Kit (Malt and Microbial)
Related Carbohydrates
P-BGBL – β-Glucan (Barley; Low Viscosity) P-BGBM – β-Glucan (Barley; Medium Viscosity) P-BGBH – β-Glucan (Barley; High Viscosity) P-BGOM – β-Glucan (Oat; Medium Viscosity) P-BGOH – β-Glucan (Oat; High Viscosity) P-BGCFA – β-Glucan CFA Standard P-MWBGS – β-Glucan MW Standards P-LICHN – Lichenan (Icelandic Moss) O-BGTRIA – 32-β-D-Glucosyl-cellobiose O-BGTRIB – 31-β-D-Cellobiosyl-glucose O-BGTETB – 31-β-D-Cellotriosyl-glucose O-BGTETC – 32-β-D-Cellobiosyl-cellobiose +
33-β-D-Glucosyl-cellotriose
Colourimetric Substrates
T-BGZ-200T – β-Glucazyme Tablets S-ABG100 – Azo-Barley Glucan I-AZBGL – AZCL-Barley β-Glucan
Associated Enzymes
E-LICACT – Non-specific endo-1,3(4)-β-Glucanase
(Clostridium thermocellum) E-LICHN – Lichenase (endo-1,3:1,4-β-D-Glucanase)
(Bacillus subtilis) E-CELTR – Cellulase (endo-1,4-β-D-glucanase)
(Trichoderma longibrachiatum)

检测方案
分析方案 K-MBG4_LICHENASE_D-CHEM2910 K-MBG4_LICHENASE_D-CHEMT K-MBG4_MALT_D-CHEM2910 K-MBG4_MALT_D-CHEMT
多个超级计算器
选择 Mega-Calc K-MBG4_LICHENASE_CALC K-MBG4_MALT_CALC

分析协议数据计算器常见问题解答

相关产品
类似产品
K-MBGL – β-葡聚糖酶检测试剂盒(麦芽和微生物)
相关碳水化合物
P-BGBL – β-葡聚糖(大麦;低粘度) P-BGBM – β-葡聚糖(大麦;中等粘度) P-BGBH – β-葡聚糖(大麦;高粘度) P-BGOM – β-葡聚糖(燕麦;中等粘度)粘度) P-BGOH – β-葡聚糖(燕麦;高粘度) P-BGCFA – β-葡聚糖 CFA 标准品 P-MWBGS – β-葡聚糖 MW 标准品 P-LICHN – 地衣(冰岛苔藓) O-BGTRIA – 32-β- D-葡萄糖基-纤维二糖 O-BGTRIB – 31-β-D-纤维二糖基-葡萄糖 O-BGTETB – 31-β-D-纤维三糖基-葡萄糖 O-BGTETC – 32-β-D-纤维二糖基-纤维二糖 +
33-β-D-葡萄糖基纤维三糖
比色底物
T-BGZ-200T – β-Glucazyme 片剂 S-ABG100 – 偶氮大麦葡聚糖 I-AZBGL – AZCL-大麦 β-葡聚糖
相关酶
E-LICACT – 非特异性 endo-1,3(4)-β-葡聚糖酶
(热纤梭菌) E-LICHN – 地衣酶 (endo-1,3:1,4-β-D-Glucanase)
(枯草芽孢杆菌) E-CELTR – 纤维素酶 (endo-1,4-β-D-glucanase)
(长臂木霉)

Safety Information
Symbol : Not Applicable
Signal Word : Not Applicable
Hazard Statements : Not Applicable
Precautionary Statements : Not Applicable
Safety Data Sheet
Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)

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