Megazyme E-GLUKEC 葡糖酸激酶[大肠杆菌]
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中文名称：葡糖酸激酶[大肠杆菌]

英文名：Gluconokinase (Gluconate kinase)

货号：E-GLUKEC

规格：1500 Units

High purity recombinant Gluconokinase (Gluconate kinase) (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.7.1.12
CAS: 9030-55-1

gluconokinase; ATP:D-gluconate 6-phosphotransferase

Recombinant. From Escherichia coli.
In 3.2 M ammonium sulphate.
Supplied at ~ 400 U/mL.

Specific activity:
~ 180 U/mg (25oC, pH 8.0 on D-gluconic acid).

Stability: Minimum 1 year at 4oC. Check vial for details.

DESCRIPTION

Gluconokinase (Gluconate kinase) (Escherichia coli)

EC 2.7.1.12
CAS: 9030-55-1

Synonyms:
gluconokinase; ATP:D-gluconate 6-phosphotransferase

Form:
In 3.2 M Ammonium sulphate.

Stability:
Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 180 U/mg (25oC, pH 8.0 on D-gluconic acid).

Unit definition:
One Unit of gluconate kinase is defined as the amount of enzyme required to convert one μmole of D-gluconic acid to D-gluconate 6-phosphate + NADPH per minute in the presence of NADP+ in glycylglycine buffer (86 mM), pH 8.0 at 25oC.

Specificity:
Catalyses the reaction:
ATP + D-gluconate = ADP + 6-phospho-D-gluconate

Applications:
Applications for the measurement of D-gluconate or D-glucono-δ-lactone in the food, fermentation, wine, beverage and dairy industries.