Megazyme K-LMAL-116A L-苹果酸检测试剂盒中文说明书

L-苹果酸检测试剂盒
英文名：L-Malic Acid Assay Kit (Manual Format)
货号：K-LMAL-116A
规格：116 assays per kit

L-苹果酸(常规)试剂盒，用于饮料和食品中L-苹果酸(L-苹果酸)的特异性测定。

手动格式紫外分光光度法测定食品饮料和其他材料中L-苹果酸的含量

原则：
(L-苹果酸脱氢酶)
(1)L-苹果酸+NAD⁺ 草酰乙酸↔+NADH+H⁺

(谷氨酸-草酰乙酸转氨酶)
(2)草酸盐+L-谷氨酸→L-天冬氨酸+2-氧戊二酸

Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

优势

• PVP对单宁的抑制作用
• 这两种酶都是作为稳定的悬浮液供应的。
• 极具竞争力的价格(每次测试成本)
• 所有试剂在制备后均稳定>2年。
• 非常快速的反应(~3分钟)
• Mega-calc™软件工具可从我们的网站上获得，以便进行无麻烦的原始数据处理。
• 标准包括
• 扩展余因子稳定性
• 适用于手动和微型制版

L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.

Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials

Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD⁺ ↔ oxaloacetate + NADH + H⁺

(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate

Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK

Advantages

• PVP incorporated to prevent tannin inhibition
• Both enzymes supplied as stable suspensions
• Very competitive price (cost per test)
• All reagents stable for > 2 years after preparation
• Very rapid reaction (~ 3 min)
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing
• Standard included
• Extended cofactors stability
• Suitable for manual and microplate format