Megazyme K-AZOWAX 木聚糖酶检测试剂盒
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Megazyme K-AZOWAX 木聚糖酶检测试剂盒 Xylanase Assay Kit (Azo-Wax)
木聚糖酶(Azo-Wax)检测试剂盒
英文名：Xylanase Assay Kit (Azo-Wax)
货号：K-AZOWAX
规格：200 assays per kit
品牌：爱尔兰Megaeyme公司
自然界中，D-木糖以多糖形式如木聚糖，阿拉伯糖基木聚糖，GAX(glucuronoarabinoxylan)，木葡聚糖和XGA(xylogalacturonan)存在。一些海藻中也存在交联D-木糖，据认定一些相近多糖构成洋车前子胶的骨架。番石榴，梨，黑莓，罗甘莓，悬钩子，芦荟维拉胶，褐藻，紫锥菊，乳香树，花椰菜，菠菜，茄子，豌豆，青豆，秋葵，卷心菜和谷物中存在游离的D-木糖。D-木糖用于小肠疾病诊断中的吸收实验，如食品中营养，维生素和矿物质吸收障碍。D-木糖可以被小肠正常轻松地吸收，当患有这些疾病时，D-木糖不能被吸收，血液和尿中的含量很低。D-木糖检测可以帮助确定儿童无法增重的原因，尤其是那些进食足够的儿童。如果在多糖中, D-木糖与其他糖类的比例已知，就可以通过酸水解测定D-木糖的浓度进而定量多糖。木聚糖是一些多糖的主要部分，这些多糖水解后的可发酵糖类可用于生产生物燃料。
原理：
D-木糖的α-和β-变旋异构形式互换由木糖变旋酶（XMR）催化（1）。
在pH7.5下,β-D-木糖由β-木糖脱氢酶（β-XDH）催化，被NAD+氧化为D-木糖酸。
The Xylanase (Azo-Wax) test kit is suitable for the measurement and analysis of endo-1,4-β-D-xylanase in enzyme preparations, bread improver mixtures and animal feeds. Containing Azo-wheat arabinoxylan and a Trichoderma sp. xylanase control.
Colourimetric method for the determination of Xylanase in feed,
foodstuffs and other materials
Principle:
(β-xylanase)
(1) Azo-WAX + H2O → Azo-WAX fragments
(insoluble in aqueous alcohol) (soluble in aqueous alcohol)
Kit size: 200 assays
Method: Based on use of Azo-WAX reagent (590 nm)
Total assay time: ~ 45 min
Detection limit: 0.2 U/mL of assay solution
Application examples:
Animal feeds, enzyme preparations, bread improver mixtures and
other materials
Method recognition:
Used widely in the feed industry
Advantages
- Very cost effective
- All reagents stable for > 2 years
- Only test kit available
- Simple format
- Standard included
| Content: | 200 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Signal Response: | Increase |
| Limit of Detection: | 0.2 U/mL of assay solution |
| Reproducibility (%): | ~ 7% |
| Total Assay Time: | ~ 45 min |
| Application examples: | Animal feeds, enzyme preparations, bread improver mixtures and other materials. |
| Method recognition: | Used widely in the feed industry |
The Xylanase (Azo-Wax) test kit is suitable for the measurement and analysis of endo-1,4-β-D-xylanase in enzyme preparations, bread improver mixtures and animal feeds. Containing Azo-wheat arabinoxylan and a Trichoderma sp. xylanase control.
| 内容： | 每个试剂盒200次 |
| 船运温度： | 环境 |
| 储存温度： | 短期稳定性：2-8oC， 长期稳定性：见单个组件标签 |
| 稳定性： | >2年在建议的储存条件下 |
| 分析物： | 恩藤-1，4-β-木聚糖酶 |
| 化验格式： | 分光光度计 |
| 检测方法： | 吸光度 |
| 波长(纳米)： | 590 |
| 信号响应： | 增加 |
| 检测极限： | 0.2U/mL测定液 |
| 可重复性(%)： | ~ 7% |
| 试验总时间： | ~45分钟 |
| 应用实例： | 动物饲料，酶制剂，面包改良剂混合物和其他材料。 |
| 方法识别： | 广泛应用于饲料工业 |
木聚糖酶(偶氮蜡)试剂盒适用于测定和分析。恩藤-1，4-β-D-木聚糖酶在酶制剂、面包改良剂混合物和动物饲料中的应用.含有偶氮小麦阿拉伯木聚糖和木霉SP.木聚糖酶控制
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Related Carbohydrates
P-XYLNBE-10G – Xylan (Beechwood)
P-WAXYL – Arabinoxylan (Wheat Flour; Low Viscosity)
P-WAXYM – Arabinoxylan (Wheat Flour; Medium Viscosity)
P-WAXYH – Arabinoxylan (Wheat Flour; High Viscosity)
P-WAXYRS – Arabinoxylan (Wheat Flour; for Reducing Sugar Assays)
P-WAXYI – Arabinoxylan (Wheat Flour; Insoluble)
P-ADWAX22 – Wheat Arabinoxylan (acid debranched, 22% Ara)
P-ADWAX26 – Wheat Arabinoxylan (acid debranched, 26% Ara)
P-EDWAX30 – Wheat Arabinoxylan (enzyme debranched, 30% Ara)
P-RAXY – Arabinoxylan (Rye Flour)
O-XBI – Xylobiose
O-XTR – Xylotriose
O-XTE – Xylotetraose
O-XPE – Xylopentaose
O-XHE – Xylohexaose
O-A3X – 32-α-L-Arabinofuranosyl-xylobiose (A3X)
O-A2XX – 23-α-L-Arabinofuranosyl-xylotriose (A2XX)
O-A23XX-30MG – 23,33-di-α-L-Arabinofuranosyl-xylotriose (A2,3XX)
O-XA3XX – 33-α-L-Arabinofuranosyl-xylotetraose (XA3XX)
O-XAXXMIX-10MG – 33-α-L- plus 23-α-L-Arabinofuranosyl-xylotetraose (XA3XX/XA2XX) mixture
Colourimetric Substrates
T-XYZ-200T – Xylazyme Tablets
T-XAF-1000T – Xylazyme AF Tablets
T-XAX-200T – Xylazyme AX Tablets
S-AWAXL – Azo-Wheat Arabinoxylan (Liquid)
S-AWAXP – Azo-Wheat Arabinoxylan (Powder)
S-AXBL – Azo-Xylan (Birchwood) (Liquid)
S-AXBP – Azo-Xylan (Birchwood) (Powder)
I-AZXBW – AZCL Xylan (Birchwood)
I-AZXBE – AZCL-Xylan (Beechwood)
I-AZWAX – AZCL-Arabinoxylan (Wheat)
I-AZWAXF – AZCL-Arabinoxylan (Wheat) (fine)
I-RCLWAXF – RedCL-Arabinoxylan (Wheat) (fine)
O-PNPX2-10MG – 4-Nitrophenyl-β-xylobioside
O-PNPX3 – 4-Nitrophenyl-β-xylotrioside
O-4MUX2-5MG – 4-Methylumbelliferyl-β-xylobioside
O-4MUX3 – 4-Methylumbelliferyl-β-xylotrioside
Associated Enzymes
E-XYTR1 – endo-1,4-β-Xylanase M1 (Trichoderma viride)
E-XYTR3 – endo-1,4-β-Xylanase M3
(Trichoderma longibrachiatum)
E-XYLAA – endo-1,4-β-Xylanase (Aspergillus aculeatus)
E-XYAN4 – endo-1,4-β-Xylanase M4 (Aspergillus niger)