Megazyme K-LMAL-116A L-苹果酸检测试剂盒中文说明书
L-苹果酸检测试剂盒
英文名:L-Malic Acid Assay Kit (Manual Format)
货号:K-LMAL-116A
规格:116 assays per kit
L-苹果酸(常规)试剂盒,用于饮料和食品中L-苹果酸(L-苹果酸)的特异性测定。
手动格式紫外分光光度法测定食品饮料和其他材料中L-苹果酸的含量
原则:
(L-苹果酸脱氢酶)
(1)L-苹果酸+NAD+ 草酰乙酸↔+NADH+H+
(谷氨酸-草酰乙酸转氨酶)
(2)草酸盐+L-谷氨酸→L-天冬氨酸+2-氧戊二酸
Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK
优势
- PVP对单宁的抑制作用
- 这两种酶都是作为稳定的悬浮液供应的。
- 极具竞争力的价格(每次测试成本)
- 所有试剂在制备后均稳定>2年。
- 非常快速的反应(~3分钟)
- Mega-calc™软件工具可从我们的网站上获得,以便进行无麻烦的原始数据处理。
- 标准包括
- 扩展余因子稳定性
- 适用于手动和微型制版
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Manual format UV-method for the determination of L-Malic Acid in foodstuffs, beverages and other materials
Principle:
(L-malate dehydrogenase)
(1) L-Malic acid + NAD+ ↔ oxaloacetate + NADH + H+
(glutamate-oxaloacetate transaminase)
(2) Oxaloacetate + L-glutamate → L-aspartate + 2-oxoglutarate
Kit size: (K-LMALR)
58 assays (manual) / 580 (microplate) or
(K-LMALL)
116 assays (manual) / 1160 (microplate)
Method: Spectrophotometric at 340 nm
Reaction time: ~ 3 min
Detection limit: 0.25 mg/L
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables,
bread, cosmetics, pharmaceuticals and other materials (e.g. biological
cultures, samples, etc.)
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC,
EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN and MEBAK
Advantages
- PVP incorporated to prevent tannin inhibition
- Both enzymes supplied as stable suspensions
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Very rapid reaction (~ 3 min)
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Extended cofactors stability
- Suitable for manual and microplate format
Content: | (K-LMAL-58A) 58 assays (manual) / 580 assays (microplate) (K-LMAL-116A) 116 assays (manual) / 1160 assays (microplate) |
Shipping Temperature: | Ambient |
Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | L-Malic Acid |
Assay Format: | Spectrophotometer, Microplate |
Detection Method: | Absorbance |
Wavelength (nm): | 340 |
Signal Response: | Increase |
Linear Range: | 0.5 to 30 µg of L-malic acid per assay |
Limit of Detection: | 0.25 mg/L |
Reaction Time (min): | ~ 3 min |
Application examples: | Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
Method recognition: | Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN, NBN, ISO and MEBAK |
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
内容: | (K-LMAL-58A) 58项试验(手册)/580项试验(微板) (K-LMAL-116 A) 116次化验(手册)/1160次试验(微版) |
船运温度: | 环境 |
储存温度: | 短期稳定性:2-8oC, 长期稳定性:见单个组件标签 |
稳定性: | >2年在建议的储存条件下 |
分析物: | L-苹果酸 |
化验格式: | 分光光度计,微版 |
检测方法: | 吸光度 |
波长(纳米): | 340 |
信号响应: | 增加 |
线性范围: | 0.5至30克L-苹果酸每次测定 |
检测极限: | 0.25毫克/升 |
反应时间(最低): | ~3分钟 |
应用实例: | 葡萄酒、啤酒、果汁、软饮料、糖果、水果和蔬菜、面包、化妆品、药品和其他材料(例如生物培养物、样品等)。 |
方法识别: | 基于这一原则的方法已被AOAC、EEC、EN、NF、Nen、DIN、Gost、OIV、IFU、AIJN、NBN、ISO和MEBAK所接受。 |
L-苹果酸(常规)试剂盒,用于饮料和食品中L-苹果酸(L-苹果酸)的特异性测定。
内容说明:如果将所有卷减少一半,每个试剂盒的手工测试次数就会增加一倍。这可以很容易地使用MegaQuant来解决。TM波分光光度计D-MQWAVE).