Megazyme K-MBG4 麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒
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中文名称：麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒
英文名：Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
货号：K-MBG4
规格：100 assays (manual) / 400 assays (auto-analyser)
品牌： Megazyme
Megazyme K-MBG4 麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒
Malt β-glucanase:
100 assays (manual format) / 400 assays (auto-analyser format)
Lichenase:
100 / 200 assays (manual format) / 330 assays (auto-analyser format)
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase. Mixed linkage
β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g.Trichoderma sp.) but this does not result in an increase in absorbance.
Data calculators are located in the Documentation tab.
Colourimetric method for the determination of endo-1,3:1.4-β-glucanase/lichenase in crude malt extracts and enzyme preparations
Principle:
(endo-1,3:1,4-β-glucanase/lichenase)
(1) Benzylidene-BGTETB-β-CNP + H2O → Benzymidene-BGTETB + CNP
(tris buffer)
(2) CNP → phenolate ion (yellow colour)
Note: CNP = 2-Chloro-4-nitrophenol
Kit size:
Malt β-glucanase 100 assays (manual) / 400 (auto-analyser)
or
Lichenase 100 / 200 assays (manual) / 330 (auto-analyser)
Method: Spectrophotometric at 400 nm
Total assay time:
Malt β-glucanase 20 min (manual) / 10 min (auto-analyser)
or
Lichenase 10 min (manual) / 10 min (auto-analyser)
Detection limit:
Malt β-glucanase 4.3 x 10-4 U/mL
or
Lichenase 9.1 x 10-5 U/mL
Application examples:
Crude malt extracts, industrial enzyme preparations
Method recognition: Novel method
Megazyme K-MBG4 麦芽β-葡聚糖酶/β-葡聚糖酶检测试剂盒
详细产品列表：
| 产品 | 规格 | 货号 |
| Available Carbohydrates/Dietary Fiber Assay Kit | 100 assays of each component | K-ACHDF |
| Integrated Total Dietary Fiber Assay Kit | 100 assays per kit | K-INTDF |
| Rapid Integrated Total Dietary Fiber Assay Kit | 100 assays per kit | K-RINTDF |
| Total Dietary Fiber Controls | Sufficient for 6 Controls | K-TDFC |
| Starch Damage Assay Kit | 200 assays per kit | K-SDAM |
| Resistant Starch Assay Kit | 100 assays per kit | K-RSTAR |
| Resistant Starch Control Flours | 5 Controls per kit | K-RSTCL |
| Total Starch HK Assay Kit | 100 assays per kit | K-TSHK |
| Total Starch Control Flours | 5 Controls per kit | K-TSCK |
| L-Arabinose/D-Galactose Assay Kit | 115 assays (manual) / 1150 assays (microplate) | K-ARGA |
| D-Fructose/D-Glucose Assay Kit | 110 assays (manual) / 1100 assays (microplate) | K-FRUGL |
| D-Fructose/D-Glucose Assay Kit (Liquid Ready Reagents) | 1100 assays (microplate) | K-FRGLQR |
| The D-Fructose and D-Glucose Assay Kit (MegaQuant™ Format) | 60 assays per kit | K-FRGLMQ |
| L-Fucose Assay Kit | 100 assays (manual) / 1000 assays (microplate) | K-FUCOSE |
| D-Gluconic Acid/D-Glucono-δ-lactone Assay Kit | 60 assays (manual) / 600 assays (microplate) | K-GATE |
| D-Glucosamine Assay Kit | 50 assays (manual) / 500 assays (microplate) | K-GAMINE |
| D-Glucose Assay Kit (GOPOD Format) | 660 assays per kit | K-GLUC |
| α-Amylase SD Assay Kit (High Sensitivity Method) | 160 / 320 assays (manual) / 640 assays (auto-analyser) | K-AMYLSD |
| β-Amylase Assay (Betamyl-3) | 100 / 200 assays per kit | K-BETA3 |
| Catalase Assay Kit | 100 / 200 assays per kit | K-CATAL |
| Cellulase Assay Kit (CellG3 Method) | 180 / 360 assays per kit / 720 (auto-analyser) | K-CellG3 |
| Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method) | 100 assays (manual) / 400 assays (auto-analyser) | K-MBG4 |
| β-Glucanase Assay Kit (Malt and Microbial) | 100 assays per kit | K-MBGL |
| Glucose Oxidase Assay Kit | 200 assays (manual) / 2000 assays (microplate) / 1960 assays (auto-analyser) | K-GLOX |
| α-Glucuronidase Assay Kit | 50 assays (manual) / 200 assays (microplate) | K-AGLUA |
| Malt Amylase Assay Kit | 100 assays (50 of each) per kit | K-MALTA |
| Pullulanase/Limit-Dextrinase Assay Kit (PullG6 Method) | 100 / 200 assays per kit | K-PullG6 |
| Tubes for MegaQuant™ Wave Spectrophotometer (Chemistry Analyzer) | 294 tubes (1 pack) | D-SFTUBE |
| MegaQuant Colorimeter Tubes | 24 tubes | D-MQTUB |
| Kit Component Organiser | 1 Unit | D-KITORGANISER |
库存信息随时变更，准确货期以下单时确认为准。
图片仅供参考,请以实物为准。
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