Megazyme R-CELLFLR Cellafluor试剂
中文名称：Cellafluor试剂
英文名：Cellafluor Reagent
货号：R-CELLFLR
规格：100 assays (manual) / 1000 assays (auto-analyser)
Cellafluor is a fluorimetric reagent that can be used for the assay of cellulase (endo-1,4-β-glucanase). The reagent consists of 2 components:
Cellafluor是一种荧光试剂，可用于测定纤维素酶（-1,4-β-葡聚糖内切酶）。 该试剂包含2个成分：
1）4,6-O-亚苄基-4-甲基伞形酮基-β-纤维二糖苷
2）β-葡萄糖苷酶（Thermotoga maritima）
与纤维素酶（内切-1,4-β-葡聚糖酶）一起温育后，功能化的三糖被裂解，辅助的β-葡萄糖苷酶可以起到水解含有4-甲基伞形酮基的片段的作用。 4-甲基伞形酮的形成速率与纤维素酶（内切1,4-β-葡聚糖酶）的活性成正比。 终止测定反应，并通过添加Tris缓冲液从荧光团获得全荧光。
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | endo-Cellulase |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Fluorescence |
| Wavelength (nm): | 365 (ex), 450 (em) |
| Reproducibility (%): | ~ 3% |
Cellafluor is a fluorimetric reagent that can be used for the assay of cellulase (endo-1,4-β-glucanase). The reagent consists of 2 components:
1) 4,6-O-Benzylidene-4-methylumbelliferyl-β-cellotrioside
2) β-glucosidase (Thermotoga maritima)
Upon incubation with a cellulase (endo-1,4-β-glucanase) enzyme, the functionalised trisaccharide is cleaved and the ancillary β-glucosidase can act to hydrolyse the fragment containing the 4-methylumbelliferyl group. The rate of formation of 4-methylumbelliferyl is directly proportional to the cellulase (endo-1,4-β-glucanase) activity. The assay reaction is terminated and full fluorescence obtained from the fluorophore by the addition of Tris buffer.
| 内容： | 100次分析(手册)/1000次分析(微版)/1000次分析(自动分析仪) |
| 船运温度： | 环境 |
| 储存温度： | 短期稳定性：2-8oC， 长期稳定性：见单个组件标签 |
| 稳定性： | >2年在建议的储存条件下 |
| 分析物： | 恩藤-纤维素酶 |
| 化验格式： | 分光光度计，微板，自动分析仪 |
| 检测方法： | 荧光 |
| 波长(纳米)： | 365(Ex)，450(Em) |
| 可重复性(%)： | ~ 3% |
Cellafluor是一种荧光试剂，可用于纤维素酶的测定。恩藤-1，4-β-葡聚糖酶.该试剂由2个组分组成：
1) 4,6-O-Benzylidene-4-methylumbelliferyl-β-cellotrioside
2)β-葡萄糖苷酶([医]金龟子)
用纤维素酶孵育(恩藤-1，4-β-葡聚糖酶，功能化的三糖被裂解，辅助的β-葡萄糖苷酶能水解含有4-甲基伞形伞形基的片段.4-甲基伞形虫酰基的形成速度与纤维素酶成正比。恩藤-1，4-β-葡聚糖酶活性。加入Tris缓冲液，从荧光团中终止测定反应，得到全荧光。
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S-ACMCL – Azo-CM-Cellulose (Liquid)
I-ACELL – Azo-α-Cellulose
Related Carbohydrates
P-CMC4M – Carboxymethyl Cellulose 4M
P-BGBL – β-Glucan (Barley; Low Viscosity)
P-BGBM – β-Glucan (Barley; Medium Viscosity)
P-BGBH – β-Glucan (Barley; High Viscosity)
P-BGOM – β-Glucan (Oat; Medium Viscosity)
P-BGOH – β-Glucan (Oat; High Viscosity)
P-BGCFA – β-Glucan CFA Standard
P-GLCML – Glucomannan (Konjac; Low Viscosity)
P-GLCMH – Glucomannan (Konjac; High Viscosity)
P-XYGLN – Xyloglucan (Tamarind)
O-CTR-50MG – Cellotriose
O-CTE-50MG – Cellotetraose
O-CPE-20MG – Cellopentaose
O-CHE – Cellohexaose
O-CTRRD – 1,4-β-D-Cellotriitol (borohydride reduced)
O-CTERD – 1,4-β-D-Cellotetraitol (borohydride reduced)
O-CPERD – 1,4-β-D-Cellopentaitol (borohydride reduced)
O-CHERD – 1,4-β-D-Cellohexaitol (borohydride reduced)
Colourimetric Substrates
O-4MUG2 – 4-Methylumbelliferyl-β-cellobioside
O-4MUG3 – 4-Methylumbelliferyl-β-cellotrioside
O-4MUG4 – 4-Methylumbelliferyl-β-cellotetraoside
O-4MUG5 – 4-Methylumbelliferyl-β-cellopentaoside
O-CPNPG2-100 – 2-Chloro-4-nitrophenyl-β-cellobioside
O-CPNPG3-50 – 2-Chloro-4-nitrophenyl-β-cellotrioside
O-CPNPG4-50 – 2-Chloro-4-nitrophenyl-β-cellotetraoside
O-CPNPG5 – 2-Chloro-4-nitrophenyl-β-cellopentaoside
O-BNAPG3 – Blocked β-naphthyl-β-cellotrioside
Associated Enzymes
E-CELAN – Cellulase (endo-1,4-β-D-glucanase)
(Aspergillus niger)
E-CELBA – Cellulase (endo-1,4-β-D-glucanase)
(Bacillus amyloliquefaciens)
E-CELTE – Cellulase (endo-1,4-β-D-glucanase)
(Talaromyces emersonii)