This test kit is suitable for the measurement and analysis of Total Dietary Fiber as per Codex Alimentarius definition, updated to be more consistent with in vivo conditions in the human small intestine, i.e. a 4 h incubation time. Under these conditions more accurate measurement of resistant starch is obtained, including phosphate cross-liked starch (RS4). Use of higher enzyme concentrations ensures that resistant maltodextrins produced from non-resistant starch under the incubation conditions of the Integrated Total Dietary Fiber procedure (AOAC Methods 2009.01 and 2011.25 are no longer produced.
In this rapid, improved method, the incubation time with PAA + AMG is reduced to 4 h and the levels of both PAA and AMG are increased to ensure that resistant starch levels obtained with a set of control samples are consistent with ileostomy data. Under these conditions, the DF values obtained for most samples are the same as those obtained with AOAC Methods 2009.01 and 2011.25.
The dietary fiber fractions that are measured with this method are:
1. High Molecular Weight Dietary Fiber (HMWDF) including Insoluble Dietary Fiber (IDF) and Low Molecular Weight Soluble Dietary Fiber (SDFS; soluble dietary fiber which is soluble in the presence of 78% aqueous ethanol), or
2. Insoluble dietary fiber (IDF), Higher Molecular Weight Soluble Dietary Fiber (SDFP; water soluble dietary fiber that precipitates in the presence of 78% aqueous ethanol) and SDFS.
The enzymes used in this method are high purity and effectively devoid of contaminating enzymes active on other dietary fiber components such as β-glucan, pectin and arabinoxylan. They are supplied as freeze-dried powders; allowing the use of glycerol as an internal standard.

For the determination of Total Dietary Fiber in cereal products, foodstuffs, feeds and other materials. 

Principle:
(Pancreatic α-amylase + amyloglucosidase)
(1) Non-resistant starch + H₂O → D-glucose

(protease)
(2) Protein + H₂O → peptides

(3) IDF (including resistant starch) and alcohol precipitated 
soluble DF (SDFP) determined gravimetrically

(4) Alcohol soluble DF (SDFS) determined by HPLC

(5) Ash and residual protein determined on DF residues 
and subtracted

Kit size:                             100 assays
Method:                              Hydrolysis/removal of non-dietary 
fibre components
Total assay time:                ~ 3 h work (over 1-2 days)
Detection limit:                   0.5-100% of sample weight
Application examples: 
Food ingredients, food products and other materials
   

* See McCleary, B. V., Sloane, N & Draga, A. (2015). Determination of total dietary fibre and available carbohydrates: a rapid integrated procedure that simulates in vivo digestion. Starch /Starke, 66, 1-24.

Advantages

• More rapid measurement – incubation time with PAA + AMG reduced to 4 h in comparison with AOAC 2009.01 (increased levels of enzyme employed)
• DF values for most samples are very similar to those obtained with AOAC Method 2009.01
• Rapid Integrated Total Dietary Fiber method removes all of the limitations that have been identified with AOAC Method 2009.01*
• All reagents stable for > 2 years after preparation
• The method is consistent with the CODEX Alimentarius definition of dietary fiber
• Mega-Calc™ software tool is available from our website for hassle-free raw dataprocessing
• Very competitive price (cost per test)

该测试试剂盒适合按照食品法典的定义进行总膳食纤维的测量和分析，并更新为与人小肠的体内条件更一致，即孵育时间为4小时。在这些条件下，可以获得更准确的抗性淀粉测量值，包括磷酸盐类十字淀粉（RS4）。使用较高的酶浓度可确保在综合总膳食纤维程序的孵育条件下（AOAC方法2009.01和2011.25）不再生产由非抗性淀粉产生的抗性麦芽糊精。
在这种快速，改进的方法中，与PAA + AMG的孵育时间缩短至4 h，同时提高了PAA和AMG的水平，以确保通过一组对照样品获得的抗性淀粉水平与回肠造口术数据一致。在这些条件下，大多数样品获得的DF值与AOAC方法2009.01和2011.25获得的DF值相同。
用这种方法测量的膳食纤维比例为：
1.高分子量膳食纤维（HMWDF），包括不溶性膳食纤维（IDF）和低分子量可溶性膳食纤维（SDFS；在78％乙醇水溶液中可溶的可溶性膳食纤维），或
2.不溶性膳食纤维（IDF），高分子量可溶性膳食纤维（SDFP；在78％的乙醇水溶液中沉淀的水溶性膳食纤维）和SDFS。
此方法中使用的酶具有很高的纯度，并且有效地消除了对其他膳食纤维成分（例如β-葡聚糖，果胶和阿拉伯木聚糖）具有活性的污染酶。它们以冻干粉的形式提供；允许使用甘油作为内标。

用于测定谷物产品，食品，饲料和其他材料中的总膳食纤维。

原则：
（胰腺α-淀粉酶+淀粉葡糖苷酶）
（1）非抗性淀粉+ H2O→D-葡萄糖

（蛋白酶）
（2）蛋白质+ H2O→肽

（3）IDF（包括抗性淀粉）和酒精沉淀
重量法测定可溶性DF（SDFP）

（4）HPLC测定的醇溶性DF（SDFS）

（5）根据DF残留物测定的灰分和残留蛋白质
并减去

试剂盒大小：100个测定
方法：水解/去除非饮食
纤维成分
总测定时间：〜3小时工作（1-2天以上）
检测极限：样品重量的0.5-100％
应用实例：
食品成分，食品和其他材料

*参见McCleary，B.V.，Sloane，N和Draga，A.（2015年）。膳食纤维总量和可用碳水化合物的测定：一种模拟体内消化的快速综合程序。淀粉/ Starke，66，1-24。

好处

测量更快-与AOAC 2009.01相比，PAA + AMG的孵育时间缩短至4小时（增加的酶水平）
大多数样品的DF值与AOAC方法2009.01获得的值非常相似
快速综合总膳食纤维方法消除了AOAC方法2009.01 *所确定的所有限制
制备后所有试剂均稳定> 2年
该方法与CODEX食品纤维的定义一致
可从我们的网站上获得Mega-Calc™软件工具，以进行无忧的原始数据处理
极具竞争力的价格（每次测试费用）