分类目录归档：Megazyme生物酶试剂

Megazyme E-PULKP 普鲁兰酶M1[植生克雷伯氏菌] Pullulanase M1 (K. planticola)

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Megazyme E-PULKP 普鲁兰酶M1[植生克雷伯氏菌] Pullulanase M1 (K. planticola)
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中文名称：普鲁兰酶M1[植生克雷伯氏菌]

英文名：Pullulanase M1 (Klebsiella planticola)

货号：E-PULKP

规格：700 Units

 

Pullulanase M1 (Klebsiella planticola)

High purity Pullulanase M1 (Klebsiella planticola) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7 

pullulanase; pullulan 6-alpha-glucanohydrolase

Highly purified. From Klebsiella planticola. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.
Supplied at ~ 700 U/mL.

Specific activity:
~ 30 U/mg (40oC, pH 5.0 on pullulan).

Stability: > 4 years at 4oC.

Recommended pullulanase for research on starch structure.

DESCRIPTION

Pullulanase M1 (Klebsiella planticola)

EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7 

Synonyms:
pullulanase; pullulan 6-alpha-glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 4 years at 4oC.

Specific activity:
~ 30 U/mg (40oC, pH 5.0 on pullulan).

Unit definition:
One Unit of pullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC.

Specificity:
Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen.

Applications:
Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups.

Megazyme E-PULBL 普鲁兰酶M2[对地衣芽孢杆菌]

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Megazyme E-PULBL 普鲁兰酶M2[对地衣芽孢杆菌]
Megazyme代理
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中文名称：普鲁兰酶M2[对地衣芽孢杆菌]

英文名：Pullulanase M2 (Bacillus licheniformis)

货号：E-PULBL

规格：2000 Units

Pullulanase M2 (Bacillus licheniformis)

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High purity Pullulanase M2 (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7

pullulanase; pullulan 6-alpha-glucanohydrolase

Highly purified. From Bacillus licheniformis. Electrophoretically homogeneous (MW 113,000).
In 3.2 M ammonium sulphate.
Supplied at ~ 900 U/mL.

Specific activity:
~ 40 U/mg (40oC, pH 5.0 on pullulan).

Stability: Minimum 1 year at 4oC. Check vial for details.

DESCRIPTION

Pullulanase M2 (Bacillus licheniformis)

EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7

Synonyms:
pullulanase; pullulan 6-alpha-glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability:
Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 40 U/mg (40oC, pH 5.0 on pullulan).

Unit definition:
One Unit of pullulanase M2 activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC.

Specificity:
Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α and β-limit dextrins of amylopectin and glycogen.

Applications:
Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups.

 

Megazyme E-PTABS 磷酸转乙酰酶[枯草杆菌] Phosphotransacetylase (B. Subtilis)

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Megazyme E-PTABS 磷酸转乙酰酶[枯草杆菌] Phosphotransacetylase (B. Subtilis)
Megazyme代理
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中文名称：磷酸转乙酰酶[枯草杆菌]

英文名：Phosphotransacetylase (Bacillus subtilis)

货号：E-PTABS

规格：3000 Units

Phosphotransacetylase (Bacillus subtilis)

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 2.3.1.8
CAS: 9029-91-8 

phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase

Recombinant. From Bacillus subtilis.
In 3.2 M ammonium sulphate.
Supplied at ~ 3,000 U/mL.

Specific activity:
~ 13,000 U/mg (25oC, pH 7.4 on Coenzyme A).

Stability: Minimum 1 year at 4oC. Check vial for details.

DESCRIPTION

Phosphotransacetylase (Bacillus subtilis)

EC 2.3.1.8
CAS: 9029-91-8

Synonyms:
phosphate acetyltransferase; acetyl-CoA:phosphate acetyltransferase

Form:
In 3.2 M ammonium sulphate.

Stability:
Minimum 1 year at 4oC. Check vial for details.

Specific activity:
~ 13,000 U/mg (25oC, pH 7.4 on coenzyme A).

Unit definition:
One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25oC.

Specificity:
Catalyses the reaction:
Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Applications:
Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

 

Megazyme E-PLYCJ 果胶酸裂合酶M2[日本龟蜡蚧]

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Megazyme E-PLYCJ 果胶酸裂合酶M2[日本龟蜡蚧]
Megazyme代理
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中文名称：果胶酸裂合酶M2[日本龟蜡蚧]

英文名：Pectate lyase (Cellvibrio japonicus)

货号：E-PLYCJ

规格：2500 Units

Pectate lyase (Cellvibrio japonicus)

High purity recombinant Pectate lyase (Cellvibrio japonicus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 4.2.2.2
CAZy Family: PL10
CAS: 9015-75-2

pectate lyase; (1,4)-alpha-D-galacturonan lyase

Recombinant. From Cellvibrio japonicus.
In 3.2 M ammonium sulphate.
Supplied at ~ 500 U/mL.

Specific activity:
~ 500 U/mg (40oC, pH 10.0 on polygalacturonic acid).

Stability: > 4 years at 4oC.

DESCRIPTION

Pectate lyase (Cellvibrio japonicus) 

EC 4.2.2.2
CAZy Family: PL10
CAS: 9015-75-2

Synonyms:
pectate lyase; (1,4)-alpha-D-galacturonan lyase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 4 years at 4oC.

Specific activity:
~ 500 U/mg (40oC, pH 10.0 on polygalacturonic acid).

Unit definition:
One Unit of pectate lyase activity is defined as the amount of enzyme required to release one µmole of 4,5-unsaturated product per minute from polygalacturonic acid (1.25 mg/mL) in the presence of calcium chloride (1 mM) in CAPS buffer (50 mM), pH 10.0 at 40oC.

Specificity:
Eliminative cleavage of (1,4)-α-D-galacturonan to give oligosaccharides with 4-deoxy-α-D-galact-4-enuronosyl groups at their non-reducing ends.

Applications:
Applications in carbohydrate and biofuels research.

 

Megazyme E-PGM 葡糖磷酸变位酶[微生物] Phosphoglucomutase (microbial) 3000U

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Megazyme E-PGM 葡糖磷酸变位酶[微生物] Phosphoglucomutase (microbial) 3000U
Megazyme代理
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中文名称：酸变位酶（α-葡萄糖磷酸）（微生物）

英文名：Mutase (α-Phosphoglucomutase) (microbial)

货号：E-PGM

规格：3000 Units

 

Mutase (α-Phosphoglucomutase) (microbial)

High purity recombinant α-Phosphoglucomutase (microbial) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.4.2.2
CAS: 9001-81-4 

phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent); alpha-D-glucose 1,6-phosphomutase

Recombinant. From microbial source.
In 50% (v/v) glycerol.
Supplied at ~ 1,000 U/mL.

Specific activity:
~ 170 U/mg (25oC, pH 7.4 on α-D-glucose 1-phosphate).

Stability: > 2 years below -10oC.

DESCRIPTION

Mutase (α-Phosphoglucomutase)

EC 5.4.2.2
CAS: 9001-81-4

Synonyms:
phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent); alpha-D-glucose 1,6-phosphomutase

Form:
In 50% (v/v) glycerol.

Stability:
> 2 years below -10oC.

Specific activity:
~ 170 U/mg (25oC, pH 7.4 on α-D-glucose 1-phosphate).

Unit definition:
One Unit of α-Phosphoglucomutase activity is defined as the amount of enzyme required to release one μmole of NADPH per minute from NADP+ at pH 7.4 and 25oC.

Specificity:
Catalyses the reaction:
α-D-Glucose 1-phosphate = D-glucose 6-phosphate

Applications:
Applications in diagnostics and analytical methods in carbohydrate and the food and feeds, fermentation, beverages and wine industries.

Megazyme E-PGISC-5KU 磷酸葡糖异构酶[酿酒酵母]

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Megazyme E-PGISC-5KU 磷酸葡糖异构酶[酿酒酵母]
Megazyme试剂盒代理、Megazyme代理
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中文名称：磷酸葡糖异构酶[酿酒酵母]

英文名：Phosphoglucose isomerase (Saccharomyces cerevisiae)

货号：E-PGISC-50KU

规格：50000 units

Phosphoglucose isomerase (Saccharomyces cerevisiae)

High purity Phosphoglucose isomerase (Saccharomyces cerevisiae) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9
CAS: 9001-41-6

glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase

Recombinant. From Saccharomyces cerevisiae.
In 3.2M ammonium sulphate.
E-PGISC-5KU:  Supplied at ~ 1,000 U/mL.
E-PGISC-50KU:  Supplied at ~ 5,000 U/mL.

Specific activity:
~ 350 U/mg of protein (25oC, pH 7.6 on fructose 6-phosphate);
~ 570 U/mg of protein (40oC, pH 7.6 on fructose 6-phosphate).

Stability: > 4 years at 4oC.

Data booklets for each pack size are located in the Documentation tab.

DESCRIPTION

Phosphoglucose Isomerase (Saccharomyces cerevisiae)

EC 5.3.1.9
CAS: 9001-41-6

Synonyms:
glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 4 years at 4oC.

Specific activity:
~ 350 U/mg (25oC, pH 7.6 on fructose 6-phosphate);
~ 570 U/mg (40oC, pH 7.6 on fructose 6-phosphate).

Unit definition:
One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate at pH 7.6.

Specificity:
Catalyses the reaction:
D-Glucose 6-phosphate = D-fructose 6-phosphate.

Applications:
Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.

Megazyme E-PGIBS 磷酸葡糖异构酶[枯草杆菌]

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Megazyme E-PGIBS 磷酸葡糖异构酶[枯草杆菌]
Megazyme代理
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中文名称：磷酸葡糖异构酶[枯草杆菌]

英文名：Phosphoglucose isomerase (Bacillus subtilis)

货号：E-PGIBS-50KU

规格：50000 units

Phosphoglucose isomerase (Bacillus subtilis)

High purity recombinant Phosphoglucose isomerase (Bacillus subtilis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 5.3.1.9
CAS: 9001-41-6 

glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase

Recombinant. From Bacillus subtilis.
In 3.2 M ammonium sulphate.
E-PGIBS-5KU: Supplied at > 1,000 U/mL.
E-PGIBS-50KU: Supplied at ~ 3,550 U/mL.

Specific activity:
> 60 U/mg of protein (25oC, pH 7.6 on fructose 6-phosphate);
> 120 U/mg (40oC, pH 7.6 on fructose 6-phosphate).

Stability: > 4 years at 4oC.

Data booklets for each pack size are located in the Documentation tab.

DESCRIPTION

Phosphoglucose Isomerase (Bacillus subtilis)

EC 5.3.1.9
CAS: 9001-41-6 

Synonyms:
glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 4 years at 4oC.

Specific activity:
> 60 U/mg of protein (25oC, pH 7.6 on fructose 6-phosphate);
> 120 U/mg (40oC, pH 7.6 on fructose 6-phosphate).

Unit definition:
One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate at pH 7.6.

Specificity:
Catalyses the reaction:
D-Glucose 6-phosphate = D-fructose 6-phosphate.

Applications:
Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries.

 

Megazyme E-CELTH 1,3-b-D葡聚糖内切酶[木霉菌]

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Megazyme E-CELTH 1,3-b-D葡聚糖内切酶[木霉菌]

中文名称：1,3-b-D葡聚糖内切酶[木霉菌]

英文名：endo-1,3-β-D-Glucanase (Trichoderma sp.)

货号：E-CELTH

Cellulase (endo-1,4-β-D-glucanase) (Thermobifida halotolerans)

High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.4
CAZy Family: GH6
CAS: 9012-54-8

1,4-beta-D-glucan 4-glucanohydrolase

Recombinant. From Thermobifida halotolerans.
In 3.2 M ammonium sulphate.
Supplied at ~ 150 U/mL.

Specific activity:
~ 27 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 60oC;
~ 16 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 40oC.

Stability: > 4 years at 4oC.

DESCRIPTION

Cellulase (endo-1,4-β-D-glucanase) (Alkali Stable) (Thermobifida halotolerans)

EC 3.2.1.4
CAZy Family: GH6
CAS: 9012-54-8

Synonyms:
1,4-beta-D-glucan 4-glucanohydrolase

Form:
In 3.2 M Ammonium sulphate.

Stability:
> 4 years at 4oC.

Specific activity:
~ 27 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 60oC;
~ 16 U/mg protein (on CM-Cellulose 4M) at pH 8.5 and 40oC.

Unit definition:
One Unit of cellulase (endo-1,4-β-D-glucanase) activity is defined as the amount of enzyme required to release one μmole of glucose reducing-sugar equivalents per minute from CM-Cellulose 4M (10 mg/mL) in Tris buffer (50 mM), pH 8.5.

Specificity:
endo-hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose.

Applications:
For use in research.

 

Megazyme E-ISAMYHP 异淀粉酶的HP（糖原果胶6-葡聚糖水解酶）

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Megazyme E-ISAMYHP 异淀粉酶的HP（糖原果胶6-葡聚糖水解酶）

中文名称：异淀粉酶的HP（糖原果胶6-葡聚糖水解酶）

英文名： Isoamylase HP (Glycogen 6-glucanohydrolase)

货号：E-ISAMYHP

规格：200 Units

Isoamylase HP (Glycogen 6-glucanohydrolase)

High purity Isoamylase HP (Glycogen 6-glucanohydrolase) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6 

isoamylase; glycogen 6-alpha-D-glucanohydrolase

Highly purified. From Pseudomonas sp. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.
Supplied at ~ 500 U/mL.

Specific activity:
~ 240 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).

Stability: > 4 years at 4oC.

DESCRIPTION

Isoamylase HP (Glycogen 6-glucanohydrolase)

EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6 

Synonyms:
isoamylase; glycogen 6-alpha-D-glucanohydrolase

Form:
In 3.2 M ammonium sulphate.

Stability:
> 4 years at 4oC.

Specific activity:
~ 240 U/mg (40oC, pH 4.0 on oyster glycogen) (equivalent to 16 MU Sigma Units/mg).

Unit definition:
One unit of isoamylase activity is the amount of enzyme required to release one μmole of D-glucose reducing sugar equivalent in the presence of oyster glycogen per min at pH 4.0 and 40oC.

Specificity:
Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

Applications:
Applications in carbohydrate research and in the food and feeds, and cereals industry.

 

Megazyme E-PROTOF 原生质体 F Protoplast F

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Megazyme E-PROTOF 原生质体 F Protoplast F
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中文名称:原生质体F

英文名：Protoplast F

货号：E-PROTOF

规格：2.5 mL

Protoplast F

Protoplast F is a lysing enzyme mixture containing cell wall degrading enzymes, e.g. exo-1,3-β-glucanase and endo-1,3-β-glucanase, and is used for generating protoplasts of Aspergillus niger.

The enzyme mixture is supplied as a precipitate in 3.2 M ammonium sulphate.

Stability: Minimum 1 year at 4oC. Check vial for details.

DESCRIPTION

Protoplast F

Form:
The enzyme mixture is supplied as a precipitate in 3.2 M ammonium sulphate.

Stability:
Minimum 1 year at 4oC. Check vial for details.

Applications:
Protoplast F is a lysing enzyme mixture used for generating protoplasts of Aspergillus niger