Megazyme E-GLUKEC 葡糖酸激酶[大肠杆菌]
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中文名称:葡糖酸激酶[大肠杆菌]
英文名:Gluconokinase (Gluconate kinase)
货号:E-GLUKEC
规格:1500 Units
High purity recombinant Gluconokinase (Gluconate kinase) (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 2.7.1.12
CAS: 9030-55-1
gluconokinase; ATP:D-gluconate 6-phosphotransferase
Recombinant. From Escherichia coli.
In 3.2 M ammonium sulphate.
Supplied at ~ 400 U/mL.
Specific activity:
~ 180 U/mg (25oC, pH 8.0 on D-gluconic acid).
Stability: Minimum 1 year at 4oC. Check vial for details.
DESCRIPTION
Gluconokinase (Gluconate kinase) (Escherichia coli)
EC 2.7.1.12
CAS: 9030-55-1
Synonyms:
gluconokinase; ATP:D-gluconate 6-phosphotransferase
Form:
In 3.2 M Ammonium sulphate.
Stability:
Minimum 1 year at 4oC. Check vial for details.
Specific activity:
~ 180 U/mg (25oC, pH 8.0 on D-gluconic acid).
Unit definition:
One Unit of gluconate kinase is defined as the amount of enzyme required to convert one μmole of D-gluconic acid to D-gluconate 6-phosphate + NADPH per minute in the presence of NADP+ in glycylglycine buffer (86 mM), pH 8.0 at 25oC.
Specificity:
Catalyses the reaction:
ATP + D-gluconate = ADP + 6-phospho-D-gluconate
Applications:
Applications for the measurement of D-gluconate or D-glucono-δ-lactone in the food, fermentation, wine, beverage and dairy industries.